C12FDG (Synonyms: 5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside)

Name: C12FDG
Brand: MedChemExpress
SKU: HY-126839
Rating: 5.0 (9 reviews)

製品番号: HY-126839 純度: 99.82%: Data Sheet SDS COA 取扱説明書
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C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than Fluorescein di(β-D-galactopyranoside) (HY-101895) for beta-galactosidase activity determinations in animal cells (Ex/Em = 488/523 nm).

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CAS 番号 : 138777-25-0

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 1202	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 1202	在庫あり	Estimated Time of Arrival: December 31
Solid
1 mg	$200	在庫あり	Estimated Time of Arrival: December 31
5 mg	$640	在庫あり	Estimated Time of Arrival: December 31
10 mg	$1020	在庫あり	Estimated Time of Arrival: December 31
25 mg	$2000	在庫あり	Estimated Time of Arrival: December 31
50 mg	$3000	在庫あり	Estimated Time of Arrival: December 31
100 mg	$4500	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 9 publication(s) in Google Scholar

Other Forms of C12FDG:

C12FDG (solution) 在庫あり

顧客検証

Flow Cytometry

Bio/Physico-chemical Assay

Cell Imaging/Staining

: C12FDG purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Jul 11;11(28):eadw1553. [Abstract]; Immunofluorescence (IF) images of C12FDG (33 μM; 30 min)-positive and N-GSDMD–positive cells in NCI-H446 on day W8, the distribution was calculated in 174 senescent cells (scale bar, 20 μm).

: C12FDG purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Aug 20:102310. [Abstract]; Flow cytometric analysis of the expression of p16, the level of C12FDG (33 μM; 2 h, a lipophilic green fluorescent substrate used to detect the activity of β-galactosidase), and the percentage of IFN-γ in tumor-infiltrating CD8⁺ T cells from Lepr^+/+Cd4-Cre and Lepr^fl/flCd4-Cre mice aged 3–4 months injected s.c. with MC38 tumor cells (n = 5).

: C12FDG purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Sep 19:102365. [Abstract]; β-galactosidase activity (C12-FDG (33 μM; 2 h)) in AML cells treated by cytarabine at indicated concentrations for 6 days.

: C12FDG purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Dec 23:244:505-515. [Abstract]; Acceleration of cellular senescence in CMIT/MIT-exposed H441 cells. To evaluate the effect of CMIT/MIT on senescence, representative senescence markers were measured in H441 cells after 24 h of CMIT/MIT exposure. SA-β-gal activity was measured by measuring the fluorescence intensity of C12FDG (10 μM; 15 min) in CMIT/MIT-exposed H441 cells by spectrophotomete.

: C12FDG purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Dec 23:244:505-515. [Abstract]; Acceleration of cellular senescence in CMIT/MIT-exposed H441 cells. To evaluate the effect of CMIT/MIT on senescence, representative senescence markers were measured in H441 cells after 24 h of CMIT/MIT exposure. SA-β-gal activity was measured by measuring the fluorescence intensity of C12FDG (10 μM; 15 min) in CMIT/MIT-exposed H441 cells by confocal microscopy.

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製品説明

体外実験

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

1. Solution preparation^[2]
1.1 Prepare storage solution
Solvent: DMSO
Concentration: 33 mM is recommended (e.g., weigh 1 mg C12FDG and dissolve it in 35.5 μL solvent).
Storage: After aliquoting, store at -20°C or -80°C in the dark and avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 33 μM (optimized according to the experiment) with PBS (or other buffer).
Note: The working solution should be prepared and used immediately, and protected from light.

2. Fluorescent senescence-associated β-galactosidase (SA-β-Gal) assay:
2.1 Culture cells in 6-, 12- , 24-, or 96-well plates at a density of 5× 10⁵ cells/mL overnight. Incubate the cells according to your normal protocol.
2.2 Wash cells by 200 μL of PBS once and fix with 100 μL of fixation solution (2% formaldehyde/0.2% glutaraldehyde in distilled water) at room temperature for 5 min.
2.3 Wash cells by 200 μL of PBS two times, and stain with 100 μL of 33 μM C12FDG (in PBS, pH=6.0) for 10 min, and with 200 μL of Hoechst solution (1 μg/mL Hoechst 33342 (HY-15559) in PBS, pH 6.0) for 10 min.
2. 4 Image these cells by a 360-nm (Hoechst 33342) and 480-nm (C12FDG) excitation filters, and monitor through 460-nm and 535-nm emission filters, respectively.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

853.90

分子式

C₄₄H₅₅NO₁₆

Emission (Em)

523

Excitation (Ex)

488

CAS 番号

138777-25-0

Appearance

Solid

Color

White to yellow

SMILES

CCCCCCCCCCCC(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O[C@H]6[C@@H]([C@H]([C@H]([C@@H](CO)O6)O)O)O)=C5)C=C(O[C@H]7[C@@H]([C@H]([C@H]([C@@H](CO)O7)O)O)O)C=C4)OC2=O)C=C1)=O

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : 50 mg/mL (58.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1711 mL	5.8555 mL	11.7110 mL
5 mM	0.2342 mL	1.1711 mL	2.3422 mL
10 mM	0.1171 mL	0.5855 mL	1.1711 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

純度とドキュメンテーション

純度: 99.84%

Select Batch:

データシート (276 KB) SDS (394 KB)

COA (251 KB) HNMR (155 KB) RP-HPLC (160 KB) LCMS (93 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Plovins A, et al. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells. Appl Environ Microbiol. 1994 Dec;60(12):4638-41. [Content Brief]

[2]. Udono M, et al. Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. Methods. 2012 Mar;56(3):383-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1711 mL	5.8555 mL	11.7110 mL	29.2774 mL
	5 mM	0.2342 mL	1.1711 mL	2.3422 mL	5.8555 mL
	10 mM	0.1171 mL	0.5855 mL	1.1711 mL	2.9277 mL
	15 mM	0.0781 mL	0.3904 mL	0.7807 mL	1.9518 mL
	20 mM	0.0586 mL	0.2928 mL	0.5855 mL	1.4639 mL
	25 mM	0.0468 mL	0.2342 mL	0.4684 mL	1.1711 mL
	30 mM	0.0390 mL	0.1952 mL	0.3904 mL	0.9759 mL
	40 mM	0.0293 mL	0.1464 mL	0.2928 mL	0.7319 mL
	50 mM	0.0234 mL	0.1171 mL	0.2342 mL	0.5855 mL

C12FDG Related Classifications

Others

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C12FDG138777-25-05-Dodecanoylaminofluorescein di-β-D-GalactopyranosideFluorescent DyeInhibitorinhibitorinhibit

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C12FDG (Synonyms: 5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside)

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Other Forms of C12FDG:

C12FDG 関連抗体

顧客検証

おすすめ

•関連小分子:

•関連タンパク質:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

C12FDG 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

C12FDG Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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