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  4. C12FDG

C12FDG  (Synonyms: 5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside)

Cat. No.: HY-126839 Purity: 99.82%
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SDS
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C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than Fluorescein di(β-D-galactopyranoside) (HY-101895) for beta-galactosidase activity determinations in animal cells (Ex/Em = 488/523 nm).

For research use only. We do not sell to patients.

CAS No. : 138777-25-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of C12FDG:

C12FDG Related Antibodies

Top Publications Citing Use of Products

    C12FDG purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Jul 11;11(28):eadw1553.  [Abstract]

    Immunofluorescence (IF) images of C12FDG (33 μM; 30 min)-positive and N-GSDMD–positive cells in NCI-H446 on day W8, the distribution was calculated in 174 senescent cells (scale bar, 20 μm).

    C12FDG purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Aug 20:102310.  [Abstract]

    Flow cytometric analysis of the expression of p16, the level of C12FDG (33 μM; 2 h, a lipophilic green fluorescent substrate used to detect the activity of β-galactosidase), and the percentage of IFN-γ in tumor-infiltrating CD8+ T cells from Lepr+/+Cd4-Cre and Leprfl/flCd4-Cre mice aged 3–4 months injected s.c. with MC38 tumor cells (n = 5).

    C12FDG purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Sep 19:102365.  [Abstract]

    β-galactosidase activity (C12-FDG (33 μM; 2 h)) in AML cells treated by cytarabine at indicated concentrations for 6 days.

    C12FDG purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Dec 23:244:505-515.  [Abstract]

    Acceleration of cellular senescence in CMIT/MIT-exposed H441 cells. To evaluate the effect of CMIT/MIT on senescence, representative senescence markers were measured in H441 cells after 24 h of CMIT/MIT exposure. SA-β-gal activity was measured by measuring the fluorescence intensity of C12FDG (10 μM; 15 min) in CMIT/MIT-exposed H441 cells by spectrophotomete.

    C12FDG purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Dec 23:244:505-515.  [Abstract]

    Acceleration of cellular senescence in CMIT/MIT-exposed H441 cells. To evaluate the effect of CMIT/MIT on senescence, representative senescence markers were measured in H441 cells after 24 h of CMIT/MIT exposure. SA-β-gal activity was measured by measuring the fluorescence intensity of C12FDG (10 μM; 15 min) in CMIT/MIT-exposed H441 cells by confocal microscopy.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than Fluorescein di(β-D-galactopyranoside) (HY-101895) for beta-galactosidase activity determinations in animal cells (Ex/Em = 488/523 nm)[1].

    In Vitro

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

    1. Solution preparation[2]
    1.1 Prepare storage solution
    Solvent: DMSO
    Concentration: 33 mM is recommended (e.g., weigh 1 mg C12FDG and dissolve it in 35.5 μL solvent).
    Storage: After aliquoting, store at -20°C or -80°C in the dark and avoid repeated freezing and thawing.
    1.2 Preparation of working solution
    Dilute to 33 μM (optimized according to the experiment) with PBS (or other buffer).
    Note: The working solution should be prepared and used immediately, and protected from light.

    2. Fluorescent senescence-associated β-galactosidase (SA-β-Gal) assay:
    2.1 Culture cells in 6-, 12- , 24-, or 96-well plates at a density of 5× 105 cells/mL overnight. Incubate the cells according to your normal protocol.
    2.2 Wash cells by 200 μL of PBS once and fix with 100 μL of fixation solution (2% formaldehyde/0.2% glutaraldehyde in distilled water) at room temperature for 5 min.
    2.3 Wash cells by 200 μL of PBS two times, and stain with 100 μL of 33 μM C12FDG (in PBS, pH=6.0) for 10 min, and with 200 μL of Hoechst solution (1 μg/mL Hoechst 33342 (HY-15559) in PBS, pH 6.0) for 10 min.
    2. 4 Image these cells by a 360-nm (Hoechst 33342) and 480-nm (C12FDG) excitation filters, and monitor through 460-nm and 535-nm emission filters, respectively.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    C12FDG Related Antibodies
    Molecular Weight

    853.90

    Formula

    C44H55NO16
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    Emission (Em)

    523
    Excitation (Ex)

    488
    SMILES

    CCCCCCCCCCCC(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O[C@H]6[C@@H]([C@H]([C@H]([C@@H](CO)O6)O)O)O)=C5)C=C(O[C@H]7[C@@H]([C@H]([C@H]([C@@H](CO)O7)O)O)O)C=C4)OC2=O)C=C1)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (58.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.1711 mL 5.8555 mL 11.7110 mL
    5 mM 0.2342 mL 1.1711 mL 2.3422 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.84%

    SDS (394 KB)

    COA (251 KB) HNMR (155 KB) RP-HPLC (160 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.1711 mL 5.8555 mL 11.7110 mL 29.2774 mL
    5 mM 0.2342 mL 1.1711 mL 2.3422 mL 5.8555 mL
    10 mM 0.1171 mL 0.5855 mL 1.1711 mL 2.9277 mL
    15 mM 0.0781 mL 0.3904 mL 0.7807 mL 1.9518 mL
    20 mM 0.0586 mL 0.2928 mL 0.5855 mL 1.4639 mL
    25 mM 0.0468 mL 0.2342 mL 0.4684 mL 1.1711 mL
    30 mM 0.0390 mL 0.1952 mL 0.3904 mL 0.9759 mL
    40 mM 0.0293 mL 0.1464 mL 0.2928 mL 0.7319 mL
    50 mM 0.0234 mL 0.1171 mL 0.2342 mL 0.5855 mL
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    C12FDG Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    C12FDG138777-25-05-Dodecanoylaminofluorescein di-β-D-GalactopyranosideFluorescent DyeInhibitorinhibitorinhibit

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