Cholesterol oxidase, Microorganism  (Synonyms: ChOx)

Cholesterol oxidase, Microorganism (ChOx) is a bacterial flavin oxidase containing FAD, commonly used in biochemical research. Cholesterol oxidase catalyzes the oxidation of the C(3)-OH group of cholesterol (and other sterols) to cholest-5-en-3-one and isomerizes it to cholest-4-en-3-one^[1].

Product Information
Optimal pH: 7.0
Molecular weight: 65 kDa

Instructions
Soluble in water (solubility≥50 mg/mL), or in buffer according to specific experimental references (e.g. 1 mg/mL dissolved in 0.1 M Phosphate buffer, pH 7.0)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9028-76-6

Solid

Light yellow to yellow

[Cholesterol oxidase, Microorganism]

1.1.3.6

＞20 U/mg solid

≥80 U/mg protein

One unit is defined as the amount of enzyme that will oxidate 1 μmol of cholesterol per min at pH 7.0 at 37°C.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Loredano Pollegioni,et al. Cholesterol oxidase: biotechnological applications. FEBS J. 2009 Dec;276(23):6857-70.   [Content Brief]