2. Stem Cell/Wnt
  3. Hedgehog
  4. Ciliobrevin D

Ciliobrevin D is a cell-permeable, reversible and specific inhibitor of AAA+ ATPase motor cytoplasmic dynein. Ciliobrevin D inhibits Hedgehog (Hh) signaling and primary cilia formation. Ciliobrevin D inhibits dynein-dependent microtubule gliding and ATPase activity in vitro.

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CAS No. : 1370554-01-0

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10 mM * 1 mL in DMSO
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Based on 4 publication(s) in Google Scholar

Ciliobrevin D Related Antibodies

Top Publications Citing Use of Products

    Ciliobrevin D purchased from MedChemExpress. Usage Cited in: Exp Neurol. 2025 May 8:115285.  [Abstract]

    Effect of Ciliobrevin D (5.7 μL at 90 μM, yielding a final concentration of 15 μM), a cytoplasmic dynein inhibitor, on AAV.CAP-B10 hippocampal transduction following cerebral lateral ventricle (CLV) injection. (a-f) Representative fluorescence images from indicated hippocampal regions in C57BL/6 mice intra-cerebroventricluarly injected with AAV.CAP-B10 alone (a-c) or co-administered with Ciliobrevin D (d-f). Blue fluorescence denotes the DAPI nuclear staining.

    Ciliobrevin D purchased from MedChemExpress. Usage Cited in: Development. 2024 Jul 1;151(13):dev202476.  [Abstract]

    Whole-mount in situ hybridization analysis of cmyb/I> and flk1 expression in the AGM region of zebrafish embryos at 28 hpf after treatment with DMSO (control), with BF170 alone, with the AAA+ATPase motor cytoplasmic dynein inhibitor Ciliobrevin D (CBD, blocking cilia function) (10 μM) or co-treated with CBD and BF170 (red arrowheads indicate positive signals). Box and whisker plot shows quantification of the whole-mount in situ hybridization data. Scale bars: 100 μm.

    Ciliobrevin D purchased from MedChemExpress. Usage Cited in: Development. 2024 Jul 1;151(13):dev202476.  [Abstract]

    Confocal images of the AGM in flk1: mCherry/cmyb/I>: GFP double-transgenic line with BF170, Ciliobrevin D (CBD, 10 μM) or BF170 and CBD co-treatment at 28 hpf (flk1+cmyb/I>+ cells are indicated by white arrowheads). Scale bars: 100 μm.

    Ciliobrevin D purchased from MedChemExpress. Usage Cited in: J Virol. 2021 Feb 24;95(10):e02436-20.  [Abstract]

    Ciliobrevin D (20 μM) inhibited CSFV entry. PK-15 cells were pretreated with Ciliobrevin D (μM) and dimethyl sulfoxide (DMSO) at 37°C for 1 h and inoculated with CSFV (MOI = 10) at 4°C for 1 h and then shifted to 37°C for endocytosis. At 0, 1, 2, and 6 hpi, cell cultures were harvested for RT-qPCR.

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    Description

    Ciliobrevin D is a cell-permeable, reversible and specific inhibitor of AAA+ ATPase motor cytoplasmic dynein. Ciliobrevin D inhibits Hedgehog (Hh) signaling and primary cilia formation. Ciliobrevin D inhibits dynein-dependent microtubule gliding and ATPase activity in vitro[1][2].

    IC50 & Target

    Cytoplasmic dynein[1][2]
    In Vitro

    Cells treated with Ciliobrevin D exhibits abnormal (unfocused, multipolar, or collapsed) spindles with disrupted γ-tubulin localization in NIH-3T3 cells. Similar Ciliobrevin-induced spindle defects are observed in HeLa cells, although to a lesser extent. Ciliobrevin D addition also reversibly disrupts the pre-formed spindles of metaphase-arrested cells and reduces overall microtubule levels[1].
    .? Ciliobrevin D reversibly inhibits melanosome aggregation, but the non-cilia-disrupting derivative had no discernible effect at comparable doses. Ciliobrevin D similarly abrogates the movement of peroxisomes in Drosophila S2 cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ciliobrevin D Related Antibodies
    In Vivo

    Knockdown of Dync1h1 or inactivation of dynein 1 by Ciliobrevin D in the testis in vivo perturbs spermatogenesis. Knockdown of Dync1h1 or the use of Ciliobrevin D to inactivate dynein 1 in the testis in vivo perturbs MT organization through changes in the spatial expression of EB1, perturbs F-actin organization, and perturbs distribution of adhesion protein complexes at the BTB, leading to a loss of BTB integrity. F-actin disorganization in the seminiferous epithelium following Dync1h1 knockdown or dynein 1 inactivation by Ciliobrevin D is mediated by changes in the spatiotemporal expression of actin regulatory proteins Arp3 and Eps8[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    392.62

    Formula

    C17H8Cl3N3O2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C1=C(C=C(Cl)C=C1)Cl)/C(C#N)=C(N2)/NC3=CC(Cl)=CC=C3C2=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 5 mg/mL (12.73 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5470 mL 12.7350 mL 25.4699 mL
    5 mM 0.5094 mL 2.5470 mL 5.0940 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5470 mL 12.7350 mL 25.4699 mL 63.6748 mL
    5 mM 0.5094 mL 2.5470 mL 5.0940 mL 12.7350 mL
    10 mM 0.2547 mL 1.2735 mL 2.5470 mL 6.3675 mL
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    Ciliobrevin D Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ciliobrevin D1370554-01-0Hedgehogspindlesγ-tubulinmelanosomeaggregationperoxisomescytoplasmicdyneinATPasesInhibitorinhibitorinhibit

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