2. Stem Cell/Wnt
  3. Hedgehog
  4. Ciliobrevin D

Ciliobrevin D is a cell-permeable, reversible and specific inhibitor of AAA+ ATPase motor cytoplasmic dynein. Ciliobrevin D inhibits Hedgehog (Hh) signaling and primary cilia formation. Ciliobrevin D inhibits dynein-dependent microtubule gliding and ATPase activity in vitro.

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CAS No. : 1370554-01-0

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고객리뷰

Based on 4 publication(s) in Google Scholar

Ciliobrevin D Related Antibodies

Top Publications Citing Use of Products

    Ciliobrevin D purchased from MedChemExpress. Usage Cited in: Exp Neurol. 2025 May 8:115285.  [Abstract]

    Effect of Ciliobrevin D (5.7 μL at 90 μM, yielding a final concentration of 15 μM), a cytoplasmic dynein inhibitor, on AAV.CAP-B10 hippocampal transduction following cerebral lateral ventricle (CLV) injection. (a-f) Representative fluorescence images from indicated hippocampal regions in C57BL/6 mice intra-cerebroventricluarly injected with AAV.CAP-B10 alone (a-c) or co-administered with Ciliobrevin D (d-f). Blue fluorescence denotes the DAPI nuclear staining.

    Ciliobrevin D purchased from MedChemExpress. Usage Cited in: Development. 2024 Jul 1;151(13):dev202476.  [Abstract]

    Whole-mount in situ hybridization analysis of cmyb/I> and flk1 expression in the AGM region of zebrafish embryos at 28 hpf after treatment with DMSO (control), with BF170 alone, with the AAA+ATPase motor cytoplasmic dynein inhibitor Ciliobrevin D (CBD, blocking cilia function) (10 μM) or co-treated with CBD and BF170 (red arrowheads indicate positive signals). Box and whisker plot shows quantification of the whole-mount in situ hybridization data. Scale bars: 100 μm.

    Ciliobrevin D purchased from MedChemExpress. Usage Cited in: Development. 2024 Jul 1;151(13):dev202476.  [Abstract]

    Confocal images of the AGM in flk1: mCherry/cmyb/I>: GFP double-transgenic line with BF170, Ciliobrevin D (CBD, 10 μM) or BF170 and CBD co-treatment at 28 hpf (flk1+cmyb/I>+ cells are indicated by white arrowheads). Scale bars: 100 μm.

    Ciliobrevin D purchased from MedChemExpress. Usage Cited in: J Virol. 2021 Feb 24;95(10):e02436-20.  [Abstract]

    Ciliobrevin D (20 μM) inhibited CSFV entry. PK-15 cells were pretreated with Ciliobrevin D (μM) and dimethyl sulfoxide (DMSO) at 37°C for 1 h and inoculated with CSFV (MOI = 10) at 4°C for 1 h and then shifted to 37°C for endocytosis. At 0, 1, 2, and 6 hpi, cell cultures were harvested for RT-qPCR.

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    제품 설명

    Ciliobrevin D is a cell-permeable, reversible and specific inhibitor of AAA+ ATPase motor cytoplasmic dynein. Ciliobrevin D inhibits Hedgehog (Hh) signaling and primary cilia formation. Ciliobrevin D inhibits dynein-dependent microtubule gliding and ATPase activity in vitro[1][2].

    IC50 & Target

    Cytoplasmic dynein[1][2]
    In Vitro

    Cells treated with Ciliobrevin D exhibits abnormal (unfocused, multipolar, or collapsed) spindles with disrupted γ-tubulin localization in NIH-3T3 cells. Similar Ciliobrevin-induced spindle defects are observed in HeLa cells, although to a lesser extent. Ciliobrevin D addition also reversibly disrupts the pre-formed spindles of metaphase-arrested cells and reduces overall microtubule levels[1].
    .? Ciliobrevin D reversibly inhibits melanosome aggregation, but the non-cilia-disrupting derivative had no discernible effect at comparable doses. Ciliobrevin D similarly abrogates the movement of peroxisomes in Drosophila S2 cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ciliobrevin D Related Antibodies
    In Vivo

    Knockdown of Dync1h1 or inactivation of dynein 1 by Ciliobrevin D in the testis in vivo perturbs spermatogenesis. Knockdown of Dync1h1 or the use of Ciliobrevin D to inactivate dynein 1 in the testis in vivo perturbs MT organization through changes in the spatial expression of EB1, perturbs F-actin organization, and perturbs distribution of adhesion protein complexes at the BTB, leading to a loss of BTB integrity. F-actin disorganization in the seminiferous epithelium following Dync1h1 knockdown or dynein 1 inactivation by Ciliobrevin D is mediated by changes in the spatiotemporal expression of actin regulatory proteins Arp3 and Eps8[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    분자량

    392.62

    화학식

    C17H8Cl3N3O2
    CAS No.
    Appearance

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    Light yellow to yellow

    SMILES

    O=C(C1=C(C=C(Cl)C=C1)Cl)/C(C#N)=C(N2)/NC3=CC(Cl)=CC=C3C2=O
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    Room temperature in continental US; may vary elsewhere.
    보관
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
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    DMSO : 5 mg/mL (12.73 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

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    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5470 mL 12.7350 mL 25.4699 mL
    5 mM 0.5094 mL 2.5470 mL 5.0940 mL
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    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5470 mL 12.7350 mL 25.4699 mL 63.6748 mL
    5 mM 0.5094 mL 2.5470 mL 5.0940 mL 12.7350 mL
    10 mM 0.2547 mL 1.2735 mL 2.5470 mL 6.3675 mL
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ciliobrevin D1370554-01-0Hedgehogspindlesγ-tubulinmelanosomeaggregationperoxisomescytoplasmicdyneinATPasesInhibitorinhibitorinhibit

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