CL 316243

Name: CL 316243
Brand: MedChemExpress
SKU: HY-116771A
Rating: 5.0 (24 reviews)

Cat. No.: HY-116771A Pureté: 99.98%: Fiche technique
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CL316243 is a highly potent selective β3-adrenoceptor agonist with a EC₅₀ of 3 nM, but is an extremely poor to β1/2- receptors.CL316243 is a effective stimulant of adipocyte lipolysis and increases brown adipose tissue thermogenesis and metabolic rate. CL316243 has the potential for the treatment obesity, diabetes and urge urinary incontinence.

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CAS No. : 138908-40-4

Size	Stock	Quantité
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtenir un devis
500 mg	Obtenir un devis

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Based on 24 publication(s) in Google Scholar

Other Forms of CL 316243:

CL 316243 free acid In-stock

24 Publications Citing Use of MCE CL 316243

: CL 316243 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 4;16(1):6151. [Abstract]; Treatment with β3-selective adrenergic agonist CL-316,243 ( 1 mg/kg/day) for 10 days also resulted in a significant increase in the expression of CLDN5 as well as UCP1 in both BAT and iWAT.

: CL 316243 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 11;16(1):7414. [Abstract]; WT mice were injected with CL316,243 (1 mg/kg) intraperitoneally twice, once per 12 h.

: CL 316243 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 4;16(1):6151. [Abstract]; Western blot analysis (t, n = 3) of PGC1α and UCP1 in Cldn5^flox/flox and Cldn5^flox/flox; Fabp4-Cre mice treated with saline/CL-316,243 (1 mg/kg)

: CL 316243 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Nov 22:S2090-1232(25)00935-X. [Abstract]; Relative mRNA and protein levels of MFF and DRP1 among 3 groups. CL316243 (3mg/kg)/vehicle (methylcellulose) for 4 weeks (every 9:00 a.m., gavage).

: CL 316243 purchased from MedChemExpress. Usage Cited in: Food Res Int. 2023 Jan:163:112198.; CL316243 increases p-AMPKα/AMPKα ratio and UCP1 protein expression in LLE-treated cells.

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Voir toutes les isoformes

α adrenergic receptor β adrenergic receptor

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Description

CL316243 is a highly potent selective β3-adrenoceptor agonist with a EC₅₀ of 3 nM, but is an extremely poor to β1/2- receptors^[1].CL316243 is a effective stimulant of adipocyte lipolysis and increases brown adipose tissue thermogenesis and metabolic rate^[2]. CL316243 has the potential for the treatment obesity, diabetes and urge urinary incontinence^[3].

IC₅₀ & Target

β adrenergic receptor

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	111 3 Compound: CL-316243	Agonistic activity as cAMP accumulation in CHO cells expressing human beta-1-adrenergic receptor Agonistic activity as cAMP accumulation in CHO cells expressing human beta-1-adrenergic receptor	[PMID: 11277513]
CHO	EC₅₀	262 3 Compound: CL-316243	Agonistic activity was assessed by measurement of cAMP accumulation levels in CHO cells expressing human beta-2-adrenergic receptor Agonistic activity was assessed by measurement of cAMP accumulation levels in CHO cells expressing human beta-2-adrenergic receptor	[PMID: 11277513]
CHO	EC₅₀	1.15 3 Compound: CL-316243	Agonistic activity as cAMP accumulation in CHO cells expressing human beta-3-andrenergic receptor Agonistic activity as cAMP accumulation in CHO cells expressing human beta-3-andrenergic receptor	[PMID: 11277513]
CHO	EC₅₀	1.15 3 Compound: CL-316243	Agonistic activity as cAMP accumulation in CHO cells expressing human beta-3-andrenergic receptor Agonistic activity as cAMP accumulation in CHO cells expressing human beta-3-andrenergic receptor	[PMID: 11277513]
CHO	EC₅₀	111 3 Compound: CL-316243	Agonistic activity as cAMP accumulation in CHO cells expressing human beta-1-adrenergic receptor Agonistic activity as cAMP accumulation in CHO cells expressing human beta-1-adrenergic receptor	[PMID: 11277513]
CHO	EC₅₀	262 3 Compound: CL-316243	Agonistic activity was assessed by measurement of cAMP accumulation levels in CHO cells expressing human beta-2-adrenergic receptor Agonistic activity was assessed by measurement of cAMP accumulation levels in CHO cells expressing human beta-2-adrenergic receptor	[PMID: 11277513]
CHO	EC₅₀	262 3 Compound: CL-316243	Agonistic activity was assessed by measurement of cAMP accumulation levels in CHO cells expressing human beta-2-adrenergic receptor Agonistic activity was assessed by measurement of cAMP accumulation levels in CHO cells expressing human beta-2-adrenergic receptor	[PMID: 11277513]

In Vitro

CL 316243 displays binding affinities with IC₅₀ values of 0.6 μM and 1 μM for rat heart and rat soleus muscle respectively^[1].
CL 316243 inhibits spontaneously contracting, isolated rat detrusor strips in a concentration dependent manner with a mean concentration inhibiting 50% of maximal response of 2.65 nM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

CL316243 disodium (subcutaneously injection; 0.1 mg/kg/day; once a day; 1 weeks) elevates the mRNA and protein expression levels of UCP1 in BAT, irrespective of diet^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6J mice fed with high-fat diets (HFD; 45%-kcal fat) or a control diet (ND; 10%-kcal fat) for 14 weeks^[2]
Dosage:	0.1 mg/kg/day
Administration:	once a day; 1 weeks
Result:	Exhibited a premium effect of obesity in mice.

Masse moléculaire

465.79

Formule

C₂₀H₁₈ClNNa₂O₇

CAS No.

138908-40-4

Appearance

Solid

Color

White to yellow

SMILES

O=C(C1(C(O[Na])=O)OC2=CC=C(C[C@H](NC[C@@H](C3=CC=CC(Cl)=C3)O)C)C=C2O1)O[Na]

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Solvant et solubilité

In Vitro:

DMSO : 17.5 mg/mL (37.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 4.55 mg/mL (9.77 mM; ultrasonic and warming and adjust pH to 12 with 1 M NaOH and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1469 mL	10.7345 mL	21.4689 mL
5 mM	0.4294 mL	2.1469 mL	4.2938 mL
10 mM	0.2147 mL	1.0734 mL	2.1469 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (214.69 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Pureté et documentation

Purity: 99.98%

Select Batch:

Fiche technique (274 KB) SDS (252 KB)

COA (247 KB) HNMR (282 KB) RP-HPLC (284 KB) LCMS (236 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Bloom JD, et al. Disodium (R,R)-5-[2-[[2-(3-chlorophenyl)-2-hydroxyethyl]-amino] propyl]-1,3-benzodioxole-2,2-dicarboxylate (CL 316,243). A potent beta-adrenergic agonist virtually specific for beta 3 receptors. A promising antidiabetic and antiobesity agent.J Med Chem. 1992 Aug 7;35(16):3081-4. [Content Brief]

[2]. Shin W, et al. Impaired adrenergic agonist-dependent beige adipocyte induction in obese mice.J Vet Med Sci. 2019 Jun 6;81(6):799-807. [Content Brief]

[3]. Woods M, et al. Efficacy of the beta3-adrenergic receptor agonist CL-316243 on experimental bladder hyperreflexia and detrusor instability in the rat. J Urol. 2001 Sep;166(3):1142-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.1469 mL	10.7345 mL	21.4689 mL	53.6723 mL
H₂O / DMSO	5 mM	0.4294 mL	2.1469 mL	4.2938 mL	10.7345 mL
DMSO	10 mM	0.2147 mL	1.0734 mL	2.1469 mL	5.3672 mL
	15 mM	0.1431 mL	0.7156 mL	1.4313 mL	3.5782 mL
	20 mM	0.1073 mL	0.5367 mL	1.0734 mL	2.6836 mL
	25 mM	0.0859 mL	0.4294 mL	0.8588 mL	2.1469 mL
	30 mM	0.0716 mL	0.3578 mL	0.7156 mL	1.7891 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

CL 316243 Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CL 316243138908-40-4CL316243CL-316243Adrenergic ReceptorBeta ReceptorInhibitorinhibitorinhibit

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CL 316243

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Other Forms of CL 316243:

CL 316243 Related Antibodies

24 Publications Citing Use of MCE CL 316243

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform Adrenergic Receptor:

Activité biologique

Pureté et documentation

Références

Avis client

CL 316243 Related Antibodies

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

CL 316243 Related Classifications

Keywords:

Vos produits récemment consultés:

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