Creatine phosphokinase, Rabbit muscle  (Synonyms: CPK)

Creatine phosphokinase, Rabbit muscle (CPK) catalyzes the reversible reaction of creatine and ATP to form phosphocreatine and ADP. Creatine phosphokinase is a key enzyme for maintaining a constant ATP/ADP ratio during rapid energy turnover^[1].

Instructions
It can be dissolved and stored at 1mg/ml using 20 mM Tris (pH8.0), avoiding repeated freezing and thawing. It can also be dissolved according to the specific experimental reference methods. When diluting, it is recommended to add 0.1% albumin to the reaction buffer to avoid inactivation due to dilution.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9001-15-4

Solid

White to off-white

[Creatine phosphokinase, Rabbit muscle]

2.7.3.2

≥100 U/mg soild

One unit is defined as the amount of enzyme that will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30℃.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. McLeish MJ, et al. Relating structure to mechanism in creatine kinase. Crit Rev Biochem Mol Biol. 2005 Jan-Feb;40(1):1-20.  [Content Brief]