Dextranase, Trichoderma reesei 

Dextranase, Trichoderma reesei, glucan hydrolase, is often used in biochemical research. Dextranase can catalyze the hydrolysis of α-(1,6)-glucosidic bonds in dextran, and has a wide range of applications, such as food processing, sugar modification, agent preparation, and medicine for enhancing the activity of endocarditis antibiotics wait^[1].

Product Information
Molecular Weight: 64.6 kDa (Theoretical)
Optimum pH Range: 5.0-7.0
Activators: Co²⁺, Mn²⁺, Cu²⁺
Inhibitors: Hg²⁺, Ag²⁺, N-bromosuccinimide, I₂
Instructions
1.Dissolution: Soluble in water (1 mg/mL), fresh preparation recommended. Or dissolve in reference buffer according to specific experiments.
2.Dilution: Further dilute to 5-20 μg/mL immediately before use.
3.Enzymatic Hydrolysis: Incubate 1.9 mL of 2% dextran substrate in a 37°C water bath, add 0.1 mL of enzyme diluent. Incubate at 37°C for 30 minutes. Stop the reaction by periodically transferring 1 mL of enzyme substrate mixture to a tube containing 1 mL of dinitrosalicylic acid reagent.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9025-70-1

Solid

Light yellow to light brown

[Dextranase, Trichoderma reesei]

3.2.1.11

≥50 U/mg soild

One unit is defined as the amount of enzyme that hydrolyze 1% barley β-glucan to 1 μmol glucose per min at 40°C, pH 6.0.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Nayera A M Abdelwahed, et al. Application of statistical design for the optimization of dextranase production by a novel fungus isolated from Red Sea sponge. 3 Biotech. 2014 Oct;4(5):533-544.   [Content Brief]