2. Metabolic Enzyme/Protease
  3. Liposome
  4. DSPE-PEG 2000

DSPE-PEG 2000  (Synonyms: (C2H4O)nC42H82NO10P)

Cat. No.: HY-142979 Pureza: 99.37%
COA
SDS
Instrucciones de manejo Technical Support

DSPE-PEG 2000 is a PEG polymer containing DSPE and amine end groups. DSPE-PEG 2000 can be used to form micelles as nanoparticles for drug delivery.

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DSPE-PEG 2000

DSPE-PEG 2000 Estructura química

No. CAS : 892144-24-0

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Based on 13 publication(s) in Google Scholar

DSPE-PEG 2000 Related Antibodies

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Descripciòn

DSPE-PEG 2000 is a PEG polymer containing DSPE and amine end groups. DSPE-PEG 2000 can be used to form micelles as nanoparticles for drug delivery[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Reparation of siRNALNPs[2]:
1.DSPE-PEG2000 (0.11 mg) in chloroform (3 mL), and transfer the lipid solution to a round bottom flask after ultrasonic treatment to fully dissolve it.
2.Steam under vacuum conditions for 30 min (75 r/min, room temperature) to allow the organic solvent to completely evaporate and obtain a homogeneous lipid film.
3.Hydrate with 3 mL of enzyme free water for 10 min (100 r/min) and then homogenize with ultrasound.
4.siRNA solution was mixed with LNPs by electrostatic interaction at a weight ratio of 1:30, and concentrated by centrifugation in a Macrosep Advance filtration device (MAP001C37) (3000g/min, 4 °C).
5.The molecular weight was cut off to 10 kDa to remove the unsealed drug.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DSPE-PEG 2000 Related Antibodies
Fòrmula

(C2H4O)nC42H82NO10P
No. CAS
Appearance

Solid

Color

White to off-white

SMILES

O=C(NCCOP(O)(OC[C@](COC(CCCCCCCCCCCCCCCCC)=O)([H])OC(CCCCCCCCCCCCCCCCC)=O)=O)OCCO.[n]
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvente y solubilidad
In Vitro: 

DMSO : 50 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 25 mg/mL (ultrasonic and warming and heat to 60°C)

H2O : 25 mg/mL (ultrasonic and warming and heat to 60°C)

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureza y Documentación

Purity: 99.95%

SDS (394 KB)

COA (240 KB) HNMR (307 KB) CNMR (234 KB) RP-HPLC (146 KB)

Instrucciones de manejo (2659 KB)
Referencias
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DSPE-PEG 2000 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DSPE-PEG 2000892144-24-0(C2H4O)nC42H82NO10PLiposomedrug deliveryInhibitorinhibitorinhibit

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Nombre del producto:
DSPE-PEG 2000
Cat. No.:
HY-142979
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