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  4. Eculizumab

Eculizumab  (Synonyms: Anti-Human C5, Humanized Antibody; h5G1.1)

製品番号: HY-P9914 純度: 99.88%
COA Technical Support

Eculizumab (Anti-Human C5, Humanized Antibody) is a long-acting humanized monoclonal antibody targeted against complement C5. Eculizumab inhibits the cleavage of C5 into C5a and C5b and inhibits deployment of the terminal complement system including the formation of membrane attack complex (MAC). Eculizumab prevents anti-ganglioside antibody-mediated neuropathy in mice. Eculizumab can be used in hemolysis studies.

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CAS 番号 : 219685-50-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (100 μg)   今すぐ申し込む  
1 mg $220 在庫あり
5 mg $700 在庫あり
10 mg $1120 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

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カスタマーレビュー

Based on 4 publication(s) in Google Scholar

Eculizumab Featured Recommendations:

Eculizumab 関連抗体

Top Publications Citing Use of Products

    Eculizumab purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Nov 5;10(1):362.  [Abstract]

    Cyclic peptide Cp1, Cp3 or anti-C5 antibody (Eculizumab)-induced erythrocyte hemolysis rate quantified by hemoglobin absorbance at 540 nm, n = 3.

    Eculizumab purchased from MedChemExpress. Usage Cited in: Cell. 2025 May 29;188(11):3013-3029.e19.  [Abstract]

    The quantification of free Hb from human RBCs as indicated in vitro (n = 4). The results showed that the administration of Eculizumab (50 mg/mL; 30 min) significantly hampered the release of free Hb induced by activated complement compared with that of controls.

    Eculizumab purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Dec 8;15(1):43346.  [Abstract]

    LDH assay results with cell lysis normalized to lysis buffer-treated cells and additional AC/IC-only background absorbance subtraction. Effects of Eculizumab on U-87MG-AQP4-ECFP cells after complement and E5415A treatment. Cells were treated with active or heat-inactivated human complement (AC/IC) in combination with E5415A (10 µg/mL). In addition to E5415A + AC, complement inhibitors against C5 (Eculizumab, 30 µg/mL) were added to the complement 15 min before the application on cells.

    Eculizumab purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Dec 8;15(1):43346.  [Abstract]

    qPCR results of genes differently expressed in U-87MG-AQP4-ECFP cells after complement and E5415A treatment. Log2-fold changes of E5415A + AC/IC with or without Eculizumab (30 µg/mL; 15 min)-treated cells were compared to untreated controls. GAPDH served as a housekeeping gene, and all ΔCt values of all genes were calculated before the normalization to untreated cells.

    Eculizumab purchased from MedChemExpress. Usage Cited in: Blood Vessel Thromb Hemost. 2025 Dec 22.

    Effect of complement inhibition and FcγRII blockade on patient serum-induced platelet aggregation in HIMEA. Platelet aggregation induced by patients’ serum obtained during active disease (T2Pre) in the presence of (UFH, 100 IU/mL) was reduced by treatment with the anti-C5 antibody Eculizumab (0.35 mg/mL; 20 min), the C3 inhibitor compstatin (0.12 and 0.18 mg/mL), or the FcγRII-blocking antibody IV.3 (35 μg/mL). Results are expressed as the percentage of residual platelet reactivity relative to aggregation observed with UFH 100 IU/mL alone.

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    製品説明

    Eculizumab (Anti-Human C5, Humanized Antibody) is a long-acting humanized monoclonal antibody targeted against complement C5. Eculizumab inhibits the cleavage of C5 into C5a and C5b and inhibits deployment of the terminal complement system including the formation of membrane attack complex (MAC). Eculizumab prevents anti-ganglioside antibody-mediated neuropathy in mice. Eculizumab can be used in hemolysis studies[1][2].

    Isotype

    Human IgG2 kappa
    Recommend Isotype Controls
    Species Reactivity

    Human
    IC50 & Target

    Complement C5
    体外実験

    Eculizumab (3-100 μg/mL) completely prevents MAC deposition, terminal axonal neurofilament loss, and damage to perisynaptic Schwann cells, and inhibits the increase in MEPP frequency, asynchronous twitching of muscle fibres, and the occurrence of 'silent' NMJs, and prevents CGM3/NHS-induced loss of contraction force in a concentration-dependent manner in the in vitro MFS model[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Eculizumab 関連抗体
    体内実験

    Eculizumab (200 μg; i.v.; before intraperitoneal injection of NHS) completely prevents the development of weakness, respiratory difficulties, and almost completely prevents the occurrence of 'silent' NMJs, and preserves the normal contraction and NMJ function in the in vivo Miller Fisher syndrome (MFS) mouse model[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    Gene ID

    727  [NCBI]

    Accession
    Application

    ELISA, FACS, Functional assay
    Conjugated

    Unconjugated
    Reconsititution

    The product can be reconstituted/diluted with sterile PBS or saline.
    分子量

    145.74 kDa

    CAS 番号
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    [Eculizumab]
    輸送条件

    Shipping with dry ice.
    Formulation

    Please refer to the lot-specific COA for specific buffer information.
    保管条件

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Format
    • Human IgG2-IgG4 kappa
    Biological Activity
    • Flow cytometric analysis of 1X106 HepG2 cells with Eculizumab (HY-P9914, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG2 kappa Isotype Control (HY-P99002, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
    • Immobilized Human Complement C5 Protein (Flag Tag) can bind Eculizumab. The EC50 for this effect is 3.17 ng/mL.
    純度とドキュメンテーション

    純度: 99.88%

    COA (232 KB) SDS-PAGE (93 KB) SEC-HPLC (195 KB)

    Inhibitory Antibodies User Guide (603 KB)
    参考文献
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Eculizumab Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Eculizumab219685-50-4Anti-Human C5, Humanized Antibody h5G1.1Complement Systemhumanized monoclonal antibodycomplement C5C5aC5bmembrane attack complexMAChaemolysisInhibitorinhibitorinhibit

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    製品名:
    Eculizumab
    製品番号:
    HY-P9914
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