Emactuzumab  (Synonyms: RG 7155; RO 5509554)

Emactuzumab(RG 7155) is a specific monoclonal antibody that inhibits colonystimulating factor 1 receptor (CSF1R) activation. Emactuzumab has high affinity for CSF-1R with K_i value of 0.2 nM to blocks CSF-1R dimerization. Emactuzumab can be used for the research of several diseases, such as diffuse-type tenosynovial giant cell tumour (dt-GCT) ^[1][2].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

CSF1R/M-CSFR/CD115

Emactuzumab (RG 7155) binds to human and cynomolgus CSF-1R with high affinity (K_d=0.2 nM) to blocks CSF-1R dimerization^[2].
RG7155 (0-10 μg/mL, 7 days) potently inhibited the viability of CSF-1-differentiated macrophages with an IC₅₀ of 0.3 nM by inducing cell death^[2].
RG7155 (30 μg/mL, 6 days) induces cell death of in vitro-differentiated human M2-like macrophages^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emactuzumab(RG 7155) (i.v.; 0.1, 1, 10, 30 and 100 mg/kg; once) increases CSF-1 concentration in nonhuman primates’ peripheral blood^[2].
RG7155 (i.v.; 0, 30, and 100 mg/kg; once weekly; for 2 weeks) depletes CSF-1R⁺CD163⁺ macrophages in vivo^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1436  [NCBI]

P07333

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

144.41 kDa

1448221-67-7

Liquid

Colorless to light yellow

[Emactuzumab]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Human IgG1 kappa

• 
  Immobilized CSF1R Protein, Human (HEK293, HY-P72948) can bind Emactuzumab. The EC₅₀ for this effect is 8.427 ng/mL.
• 
  Flow cytometric analysis of 1.5X10⁶ THP-1 cells with Emactuzumab (HY-P99245, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Philippe A Cassier, et al. CSF1R inhibition with emactuzumab in locally advanced diffuse-type tenosynovial giant cell tumours of the soft tissue: a dose-escalation and dose-expansion phase 1 study. Lancet Oncol. 2015 Aug;16(8):949-56.  [Content Brief]

  [2]. Carola H Ries, et al. Targeting tumor-associated macrophages with anti-CSF-1R antibody reveals a strategy for cancer therapy. Cancer Cell. 2014 Jun 16;25(6):846-59.  [Content Brief]