FA-Leu-Gly-Pro-Ala-OH

Name: FA-Leu-Gly-Pro-Ala-OH
Brand: MedChemExpress
SKU: HY-P2661
Rating: 4.5 (0 reviews)

Cat. No.: HY-P2661 Pureza: 99.40%: Ficha de datos
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FA-Leu-Gly-Pro-Ala-OH is a furylacryloyl-terminal tetrapeptide that serves as a substrate for bacterial collagenase and spirochete metalloendopeptidase. FA-Leu-Gly-Pro-Ala-OH is specifically hydrolyzed by spirochete collagenase only at the Leu-Gly bond. FA-Leu-Gly-Pro-Ala-OH can be used to determine the equilibrium constant of peptide bond hydrolysis, and also to detect collagenase-mediated cleavage reactions via turbidimetry based on absorbance reduction.

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Síntesis de péptidos personalizados

FA-Leu-Gly-Pro-Ala-OH Estructura química

No. CAS : 78832-65-2

Tamaño	Stock	Cantidad
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Based on 1 Customer Validation

Other Forms of FA-Leu-Gly-Pro-Ala-OH:

FA-Leu-Gly-Pro-Ala-OH TFA Obtener un presupuesto

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Descripciòn

In Vitro

FA-Leu-Gly-Pro-Ala-OH (1 mM Ca²⁺; no added Ca²⁺) is hydrolyzed at the Leu-Gly bond by the partially purified metalloendopeptidase from unspeciated treponeme strain US with near-identical activity in the presence of 1 mM Ca²⁺ or without added Ca²⁺^[1].
FA-Leu-Gly-Pro-Ala-OH (5 mM; 15 min) was not cleaved by crude nematocyst extract from Velella velella, which did not exhibit detectable collagenase activity against the substrate under the tested conditions^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

476.52

Fòrmula

C₂₃H₃₂N₄O₇

No. CAS

78832-65-2

Appearance

Solid

Color

White to off-white

Sequence

FA-Leu-Gly-Pro-Ala

Sequence Shortening

FA-LGPA

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvente y solubilidad

In Vitro:

DMSO : ≥ 100 mg/mL (209.85 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0985 mL	10.4927 mL	20.9855 mL
5 mM	0.4197 mL	2.0985 mL	4.1971 mL
10 mM	0.2099 mL	1.0493 mL	2.0985 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.40%

Select Batch:

Ficha de datos (276 KB) SDS (393 KB)

COA (252 KB) RP-HPLC (251 KB) MS (223 KB) Elemental Analysis Report (111 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Mäkinen K K, et al. Hydrolysis of the Leu-Gly bond of phenylazobenzyl-oxycarbonyl-l-Pro-l-Leu-Gly-l-Pro-D-Arg (a substrate of microbial collagenases) by treponemes isolated from the subgingival plaque of periodontitis patients[J]. Current Microbiology, 1990, 20(1): 69-74.

[2]. Tassara E, et al. Proteomic Analysis and Biochemical Characterization of the Nematocyst Extract of the Hydrozoan Velella velella. Mar Drugs. 2024 Oct 12;22(10):468. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0985 mL	10.4927 mL	20.9855 mL	52.4637 mL
	5 mM	0.4197 mL	2.0985 mL	4.1971 mL	10.4927 mL
	10 mM	0.2099 mL	1.0493 mL	2.0985 mL	5.2464 mL
	15 mM	0.1399 mL	0.6995 mL	1.3990 mL	3.4976 mL
	20 mM	0.1049 mL	0.5246 mL	1.0493 mL	2.6232 mL
	25 mM	0.0839 mL	0.4197 mL	0.8394 mL	2.0985 mL
	30 mM	0.0700 mL	0.3498 mL	0.6995 mL	1.7488 mL
	40 mM	0.0525 mL	0.2623 mL	0.5246 mL	1.3116 mL
	50 mM	0.0420 mL	0.2099 mL	0.4197 mL	1.0493 mL
	60 mM	0.0350 mL	0.1749 mL	0.3498 mL	0.8744 mL
	80 mM	0.0262 mL	0.1312 mL	0.2623 mL	0.6558 mL
	100 mM	0.0210 mL	0.1049 mL	0.2099 mL	0.5246 mL