country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-P2661A

Copy Link Print Information

FA-Leu-Gly-Pro-Ala-OH TFA

FA-Leu-Gly-Pro-Ala-OH TFA is a furylacryloyl-terminal tetrapeptide that serves as a substrate for bacterial collagenase and spirochete metalloendopeptidase. FA-Leu-Gly-Pro-Ala-OH TFA is specifically hydrolyzed by spirochete collagenase only at the Leu-Gly bond. FA-Leu-Gly-Pro-Ala-OH TFA can be used to determine the equilibrium constant of peptide bond hydrolysis, and also to detect collagenase-mediated cleavage reactions via turbidimetry based on absorbance reduction.

For research use only. We do not sell to patients.

Formula: C₂₅H₃₃F₃N₄O₉
Molecular Weight:590.55

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

FA-Leu-Gly-Pro-Ala-OH TFA Chemical Structure

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

FA-Leu-Gly-Pro-Ala-OH (1 mM Ca²⁺; no added Ca²⁺) TFA is hydrolyzed at the Leu-Gly bond by the partially purified metalloendopeptidase from unspeciated treponeme strain US with near-identical activity in the presence of 1 mM Ca²⁺ or without added Ca²⁺^[1].
FA-Leu-Gly-Pro-Ala-OH (5 mM; 15 min) TFA was not cleaved by crude nematocyst extract from Velella velella, which did not exhibit detectable collagenase activity against the substrate under the tested conditions^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 590.55
Formula C₂₅H₃₃F₃N₄O₉
Sequence

FA-Leu-Gly-Pro-Ala
Sequence Shortening

FA-LGPA
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Mäkinen K K, et al. Hydrolysis of the Leu-Gly bond of phenylazobenzyl-oxycarbonyl-l-Pro-l-Leu-Gly-l-Pro-D-Arg (a substrate of microbial collagenases) by treponemes isolated from the subgingival plaque of periodontitis patients[J]. Current Microbiology, 1990, 20(1): 69-74.

[2]. Tassara E, et al. Proteomic Analysis and Biochemical Characterization of the Nematocyst Extract of the Hydrozoan Velella velella. Mar Drugs. 2024 Oct 12;22(10):468. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

Get Quote In-stock

Other size

Get Quote