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  4. FITC-Dextran (MW 10000)

FITC-Dextran (MW 10000)  (Synonyms: フルオレセインイソチオシアナート–デキストラン)

製品番号: HY-128868
COA 取扱説明書 Technical Support

FITC-Dextran (MW 10000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 10000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 10000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption.

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CAS 番号 : 60842-46-8

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5 mg $50 在庫あり
10 mg $85 在庫あり
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100 mg $400 在庫あり
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Based on 90 publication(s) in Google Scholar

Other Forms of FITC-Dextran (MW 10000):

FITC-Dextran (MW 10000) 関連抗体

Top Publications Citing Use of Products

顧客検証

Bio/Physico-chemical Assay
In Vivo Efficacy Study
In Vivo Imaging

    FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 18:e00741.  [Abstract]

    Intestinal permeability was assessed by FITC‐Dextran (0.6 mg/g; Oral gavage) assays on day 10 after DSS initiation (n = 6).

    FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Apr 15;23(1):291.  [Abstract]

    FITC-dextran (1 mg/mL; 45 min) permeability assay was performed to assess the barrier function of bEnd.3 endothelial cells.

    FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2025 Jul;68(7):2010-2027.  [Abstract]

    Blood FITC-Dextran (MW 4000) (200 mg/kg; Oral gavage; Single dose) levels. The results showed that METTL3-deficient mice presented significantly increased serum FITC levels, indicating increased mucosal permeability.

    FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jun 4;23(1):263.  [Abstract]

    Quantitative analysis of FITC in serum collected from mice in the FITC-Dextran (MW 4000) (0.6 mg/g; Oral gavage; Single dose) intestinal permeability assay.

    FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 May 9:144084.  [Abstract]

    MFI variations with time in Ch1, Ch3 and Ch5 of 10 kDa FITC-dextran (0.1 mg/mL; 0-24 h) diffusion.

    FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Redox Rep. 2024 Dec;29(1):2433393.  [Abstract]

    Intestinal permeability measured by serum fluorescein isothiocyanate FITC-Dextran (MW 4000) (0.44 mg/g; Oral gavage; Single dose) levels.

    FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2024 Jun 1:591:216897.  [Abstract]

    Fluorescence intensity of FITC-dextran (500 μg/mL; 36 h) in lower chamber of Transwell after incubation with TM-sEVs from patients with low relapse risk and high relapse risk in the upper chamber.

    FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 20;9(42):eadi0699.  [Abstract]

    Ex vivo imaging showed biodistribution of FITC-Dextran (MW 4000) (500 μg/mouse; i.v.; Single dose).

    FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2021 Oct;8(20):e2101912.  [Abstract]

    Large particle transport assays through µE‐BBB were conducted by passing of FITC‐dextran (100 µg/mL; 1 h) solution through the lumen compartment of the system and quantifying the amount of FITC‐dextran that had accumulated in the basolateral compartment.

    FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2021 Oct;8(20):e2101912.  [Abstract]

    To evaluate the effect of erythrocytes on the permeability of the BBB, isolated erythrocytes from young or old mice, supplemented with FITC‐dextran (100 μg/mL; 1 h), were circulated through the µE‐BBB.

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    製品説明

    FITC-Dextran (MW 10000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 10000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 10000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption[1][2][3].

    体外実験

    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1. Cell Marking (for cells undergoing apoptosis)[1]:
    1.1 Resuspend cells in culture medium and add Propidium Iodide (PI) and FITC-Dextran to a final concentration of 7.5 μM and 1.13 μM, respectively.
    1.2 Incubate cells at room temperature in the dark for 25 minutes.
    1.3 Centrifuge at 500 g for 10 minutes and discard the supernatant.
    1.4 Resuspend cells in culture medium and analyze using flow cytometry or fluorescence microscopy (PI: Ex=500 nm, Em=600 nm; FITC-Dextran: Ex=495 nm, Em=525 nm).
    2. Side-cell permeability assay[4]
    2.1 Add FITC-Dextran (0.1 mg/mL) to the basal medium in the upper chamber of the transwell.
    2.2 Collect the medium from the lower chamber of the transwell after 15 minutes.
    2.3 Measure the fluorescence signal (Ex=495 nm, Em=525 nm).
    2.4 Calculate the FITC-Dextran concentration based on the fluorescence intensity.
    2.5 Calculate the permeability.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    FITC-Dextran (MW 10000) 関連抗体
    体内実験

    Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    For intestinal barrier function assay[5]
    1. Fast mice for 4 h.
    2. Orally gavage mice with FITC-Dextran MW 10000 (0.6 mg/g).
    3. Measure fluorescence intensity of plasma in 4 h (excitation nm/emission 520 nm).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    10000.00(Average)

    Emission (Em)

    525
    Excitation (Ex)

    488
    CAS 番号
    Appearance

    Solid

    Color

    Yellow to brown

    SMILES

    [FITC-Dextran (MW 10000)]
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    H2O : 50 mg/mL (Need ultrasonic)

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    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

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    FITC-Dextran (MW 10000) Related Classifications

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    FITC-Dextran (MW 10000)60842-46-8Fluorescent Dyeapoptosisfluorescein-isothiocyanateblood-brainbarrierBBBpermeableInhibitorinhibitorinhibit

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    製品名:
    FITC-Dextran (MW 10000)
    製品番号:
    HY-128868
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