FM4-64

Name: FM4-64
Brand: MedChemExpress
SKU: HY-103466
Rating: 5.0 (22 reviews)

Art. -Nr.: HY-103466 Reinheit: 99.94%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

FM4-64 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM4-64 is widely used in endocytic and exospic membrane structure markers.

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CAS. Nr. : 162112-35-8

Größe	Verfügbarkeit	Menge
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	Auf Lager	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	Auf Lager	Estimated Time of Arrival: December 31
Solid
1 mg	Auf Lager	Estimated Time of Arrival: December 31
5 mg	Auf Lager	Estimated Time of Arrival: December 31
10 mg	Auf Lager	Estimated Time of Arrival: December 31
50 mg	Angebot einholen
100 mg	Angebot einholen

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This product is a controlled substance and not for sale in your territory.

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Based on 22 publication(s) in Google Scholar

Other Forms of FM4-64:

FM4-64 (solution) In-stock

22 Publications Citing Use of MCE FM4-64

Cell Imaging/Staining

: FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131. [Abstract]; FM4-64 (1 µM; 30 min). Confocal microscopy images of MG1655 stained with SYTOX (nucleic acid dye, green) and FM4‐64 (outer membrane dye, red) revealed that the SYTOX/FM4‐64 ratio increased under ENR treatment, indicating elevated membrane permeability.

: FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131. [Abstract]; FM4-64 (1 µM; 30 min). Fluorescence microscopy indicated no significant change in peptidoglycan integrity in ENR-treated ΔrecA strains compared to untreated controls.

: FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131. [Abstract]; FM4-64 (1 µM; 30 min). Fluorescence microscopy analysis using the SYTOX and FM4‐64 revealed increased cytoplasmic staining in both MG1655 and endolysin‐deficient strains (Δessd-1ΔrrrdΔrzod) under ENR stress, indicating membrane damage.

: FM4-64 purchased from MedChemExpress. Usage Cited in: Microbiome. 2024 Dec 21;12(1):272. [Abstract]; FM4-64 (20 μg/mL; 1 min). Representative images obtained from confocal laser scanning microscopy to analyze the behavior of AMP1. B. subtilis cells were treated with coumarin 343-labeled AMP1 at a concentration of 100 μM for various durations before observation. During observation, the B. subtilis cells membrane was stained with FM4-64.

: FM4-64 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 12:e2406473. [Abstract]; FM4-64 (1 µg/mL; 30 min). Confocal micrographs of vacuole staining (FM4‐64) and localization of Atg8 proteins in the GFP‐Atg8 mutant cells following the indicated treatment. DIC is Differential Interference Contrast; FM4‐64 was visualized as red fluorescent; Atg 8 proteins were visualized as green fluorescent.

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•Related Proteins:

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Beschreibung

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of FM working solution
1.1 Preparation of the stock solution
Dissolve FM in DMSO to obtain 5 mM of FM.
1.2 Preparation of FM working solution
Dilute the stock solution in HBSS to obtain 5-20 μM of FM working solution.
Note: Please adjust the concentration of FM working solution according to the actual situation.
2. Cell staining (Suspension cells)
2.1. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×10⁶/mL
2.2. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
2.3. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4. Wash twice with PBS, 5 minutes each time.
2.5. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2. Remove the coverslip from the medium and aspirate excess medium.
3.3. Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
3.4. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molekulargewicht

607.51

Formel

C₃₀H₄₅Br₂N₃

CAS. Nr.

162112-35-8

Appearance

Solid

Color

Dark gray to black

Emission (Em)

730

Excitation (Ex)

510

SMILES

CC[N+](CCC[N+]1=CC=C(/C=C/C=C/C=C/C2=CC=C(N(CC)CC)C=C2)C=C1)(CC)CC.[Br-].[Br-]

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (82.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6461 mL	8.2303 mL	16.4606 mL
5 mM	0.3292 mL	1.6461 mL	3.2921 mL
10 mM	0.1646 mL	0.8230 mL	1.6461 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Reinheit & Dokumentation

Purity: 99.94%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (243 KB) HNMR (281 KB) LCMS (149 KB)

Handling Instructions (2659 KB)

Verweise

[1]. J E Gale, et al. FM1-43 dye behaves as a permeant blocker of the hair-cell mechanotransducer channel. J Neurosci. 2001 Sep 15;21(18):7013-25. [Content Brief]

[2]. Zal T, et al. Spectral shift of fluorescent dye FM4-64 reveals distinct microenvironment of nuclear envelope in living cells. Traffic. 2006;7(12):1607-1613. [Content Brief]

[3]. Stenovec M, et al. Distinct labelling of fusion events in rat lactotrophs by FM 1-43 and FM 4-64 is associated with conformational differences. Acta Physiol (Oxf). 2007;191(1):35-42. [Content Brief]

[4]. Bolte S, et al, Satiat-Jeunemaitre B. FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells. J Microsc. 2004;214(Pt 2):159-173. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6461 mL	8.2303 mL	16.4606 mL	41.1516 mL
	5 mM	0.3292 mL	1.6461 mL	3.2921 mL	8.2303 mL
	10 mM	0.1646 mL	0.8230 mL	1.6461 mL	4.1152 mL
	15 mM	0.1097 mL	0.5487 mL	1.0974 mL	2.7434 mL
	20 mM	0.0823 mL	0.4115 mL	0.8230 mL	2.0576 mL
	25 mM	0.0658 mL	0.3292 mL	0.6584 mL	1.6461 mL
	30 mM	0.0549 mL	0.2743 mL	0.5487 mL	1.3717 mL
	40 mM	0.0412 mL	0.2058 mL	0.4115 mL	1.0288 mL
	50 mM	0.0329 mL	0.1646 mL	0.3292 mL	0.8230 mL
	60 mM	0.0274 mL	0.1372 mL	0.2743 mL	0.6859 mL
	80 mM	0.0206 mL	0.1029 mL	0.2058 mL	0.5144 mL

FM4-64 Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FM4-64162112-35-8Fluorescent Dyemembraneselectiveredfluorescentdyeendocytosisexocytosisorganelleorganization ,vesicleInhibitorinhibitorinhibit

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FM4-64

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Other Forms of FM4-64:

FM4-64 Related Antibodies

22 Publications Citing Use of MCE FM4-64

Spezialitätsempfehlungen

•Related Small Molecules:

•Related Proteins:

Biologische Aktivität

Reinheit & Dokumentation

Verweise

Kundenbewertung

FM4-64 Related Antibodies

Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

FM4-64 Related Classifications

Keywords:

Ihre kürzlich angesehenen Produkte:

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