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  3. Fluorescent Dye
  4. FM4-64

FM4-64 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM4-64 is widely used in endocytic and exospic membrane structure markers.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 162112-35-8

사이즈 가격 재고 수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
1 mg 해외재고보유
5 mg 해외재고보유
10 mg 해외재고보유
50 mg   견적 받기  
100 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 22 publication(s) in Google Scholar

Other Forms of FM4-64:

FM4-64 Related Antibodies

Top Publications Citing Use of Products

    FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131.  [Abstract]

    FM4-64 (1 µM; 30 min). Confocal microscopy images of MG1655 stained with SYTOX (nucleic acid dye, green) and FM4‐64 (outer membrane dye, red) revealed that the SYTOX/FM4‐64 ratio increased under ENR treatment, indicating elevated membrane permeability.

    FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131.  [Abstract]

    FM4-64 (1 µM; 30 min). Fluorescence microscopy indicated no significant change in peptidoglycan integrity in ENR-treated ΔrecA strains compared to untreated controls.

    FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131.  [Abstract]

    FM4-64 (1 µM; 30 min). Fluorescence microscopy analysis using the SYTOX and FM4‐64 revealed increased cytoplasmic staining in both MG1655 and endolysin‐deficient strains (Δessd-1ΔrrrdΔrzod) under ENR stress, indicating membrane damage.

    FM4-64 purchased from MedChemExpress. Usage Cited in: Microbiome. 2024 Dec 21;12(1):272.  [Abstract]

    FM4-64 (20 μg/mL; 1 min). Representative images obtained from confocal laser scanning microscopy to analyze the behavior of AMP1. B. subtilis cells were treated with coumarin 343-labeled AMP1 at a concentration of 100 μM for various durations before observation. During observation, the B. subtilis cells membrane was stained with FM4-64.

    FM4-64 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 12:e2406473.  [Abstract]

    FM4-64 (1 µg/mL; 30 min). Confocal micrographs of vacuole staining (FM4‐64) and localization of Atg8 proteins in the GFP‐Atg8 mutant cells following the indicated treatment. DIC is Differential Interference Contrast; FM4‐64 was visualized as red fluorescent; Atg 8 proteins were visualized as green fluorescent.

    • Biological Activity

    • 순도&문서

    • References

    • 고객리뷰

    제품 설명

    FM4-64 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM4-64 is widely used in endocytic and exospic membrane structure markers.

    In Vitro

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1. Preparation of FM working solution
    1.1 Preparation of the stock solution
    Dissolve FM in DMSO to obtain 5 mM of FM.
    1.2 Preparation of FM working solution
    Dilute the stock solution in HBSS to obtain 5-20 μM of FM working solution.
    Note: Please adjust the concentration of FM working solution according to the actual situation.
    2. Cell staining (Suspension cells)
    2.1. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL
    2.2. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
    2.3. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    2.4. Wash twice with PBS, 5 minutes each time.
    2.5. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    3. Cell staining (Adherent cells)
    3.1 Culture adherent cells on sterile coverslips.
    3.2. Remove the coverslip from the medium and aspirate excess medium.
    3.3. Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
    3.4. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    FM4-64 Related Antibodies
    분자량

    607.51

    화학식

    C30H45Br2N3
    CAS No.
    Appearance

    Solid

    Color

    Dark gray to black

    Emission (Em)

    730
    Excitation (Ex)

    510
    SMILES

    CC[N+](CCC[N+]1=CC=C(/C=C/C=C/C=C/C2=CC=C(N(CC)CC)C=C2)C=C1)(CC)CC.[Br-].[Br-]
    선적

    Room temperature in continental US; may vary elsewhere.
    보관

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    용액&용해도
    In Vitro: 

    DMSO : 50 mg/mL (82.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6461 mL 8.2303 mL 16.4606 mL
    5 mM 0.3292 mL 1.6461 mL 3.2921 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • 몰농도 계산기

    • 농도 희석 계산기

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    순도&문서

    Purity: 99.94%

    SDS (252 KB)

    COA (243 KB) HNMR (281 KB) LCMS (149 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6461 mL 8.2303 mL 16.4606 mL 41.1516 mL
    5 mM 0.3292 mL 1.6461 mL 3.2921 mL 8.2303 mL
    10 mM 0.1646 mL 0.8230 mL 1.6461 mL 4.1152 mL
    15 mM 0.1097 mL 0.5487 mL 1.0974 mL 2.7434 mL
    20 mM 0.0823 mL 0.4115 mL 0.8230 mL 2.0576 mL
    25 mM 0.0658 mL 0.3292 mL 0.6584 mL 1.6461 mL
    30 mM 0.0549 mL 0.2743 mL 0.5487 mL 1.3717 mL
    40 mM 0.0412 mL 0.2058 mL 0.4115 mL 1.0288 mL
    50 mM 0.0329 mL 0.1646 mL 0.3292 mL 0.8230 mL
    60 mM 0.0274 mL 0.1372 mL 0.2743 mL 0.6859 mL
    80 mM 0.0206 mL 0.1029 mL 0.2058 mL 0.5144 mL
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    FM4-64 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    FM4-64162112-35-8Fluorescent Dyemembraneselectiveredfluorescentdyeendocytosisexocytosisorganelleorganization ,vesicleInhibitorinhibitorinhibit

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    상품명:
    FM4-64
    Cat. No.:
    HY-103466
    수량:
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