House Dust Mite Extract, from D.farinae

Name: House Dust Mite Extract, from D.farinae
Brand: MedChemExpress
SKU: HY-NP159
Rating: 4.5 (0 reviews)

Cat. No.: HY-NP159: Data Sheet
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House Dust Mite Extract, from D.farinae is a house dust mite allergen extract derived from Dermatophagoides farinae. House Dust Mite Extract, from D.farinae significantly increases the levels of cytokines (IL-4, IL-5, IL-6) in bronchoalveolar lavage fluid. House Dust Mite Extract, from D.farinae upregulates the overexpression of MUC5AC. House Dust Mite Extract, from D.farinae induces allergic asthma and pulmonary inflammation.

For research use only. We do not sell to patients.

House Dust Mite Extract, from D.farinae Chemical Structure

Size	Price	Stock	Quantity
600 μg		In-stock	Estimated Time of Arrival: December 31

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MUC5AC MUC1

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

Biological Activity
Purity & Documentation
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Description

IC₅₀ & Target^[1]

AAK1

IL-6

IL-5

IL-4

In Vivo

House Dust Mite Extract, from D. farinae (100 μg/mL; inhaled via nebulizer; 8 administrations; 10 minutes per administration) induces robust allergic asthma pathology in male BALB/c mice, including significant airway hyperresponsiveness, inflammatory cell infiltration, Th2 cytokine production, immunoglobulin elevation, peribronchial fibrosis, mucus hypersecretion, and MUC5AC overexpression^[1].
House Dust Mite Extract, from D. farinae (3 μg; i.n.; 6 doses on days 0, 4, 7, 11, 14, 18) induces robust allergic pulmonary inflammation and Th2-type adaptive immune responses in WT C57BL/6 mice, while these responses are markedly impaired in Pla2g5-null mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c (5-week-old male; asthma model via nebulization challenge)^[1]
Dosage:	100 μg/mL
Administration:	inhaled via nebulizer; 8 administrations (Days 7, 14, 21, 28, 35, 42, 60, 61); 10 minutes per administration
Result:	Induced airway hyperresponsiveness with significantly higher Penh values than saline-treated control mice (values similar to those induced by D. pteronyssinus extract). Significantly increased total cell (~110 × 10⁴/m), eosinophil (~40 × 10⁴/m), neutrophil (~15 × 10⁴/m), lymphocyte (~10 × 10⁴/m), and macrophage (~50 × 10⁴/m) counts in bronchoalveolar lavage fluid compared to saline-treated controls. Significantly elevated serum IgE (OD ~0.2), IgG1 (OD ~0.15), and IgG2a (OD ~0.12) levels compared to saline-treated controls. Significantly increased BALF cytokine levels: IL-4 (~150 pg/mL), IL-5 (~150 pg/mL), IL-6 (~40 pg/mL), IL-10 (~75 pg/mL), IL-13 (~150 pg/mL), and IL-17 (~180 pg/mL) compared to saline-treated controls. Induced pathological lung changes including thickened bronchial mucosa, inflammatory cell infiltration, lumen stenosis, mild peribronchial collagen deposition, increased goblet cell hyperplasia (PAS-positive cell count ~45% of airway cells), and elevated MUC5AC expression (fold change ~8 relative to PBS control for protein, ~8 relative to PBS control for mRNA) compared to saline-treated controls.

Animal Model:	C57BL/6 wild-type (WT) (7-9 weeks old); Pla2g5-null (7-9 weeks old)^[2]
Dosage:	3 μg
Administration:	i.n.; 6 doses on days 0, 4, 7, 11, 14, 18
Result:	Induced significant increases in total bronchoalveolar lavage (BAL) fluid cell numbers and eosinophils in WT mice. Showed 14 bronchovascular bundles (BVBs) with cellular infiltrates per 15 BVBs, and 47 mucus-producing goblet cells per mm of bronchial basal lamina in WT mice. Increased mRNA expression of IL-5, IL-13, Muc5ac, and Clca3/Gob-5 in lung tissue of WT mice relative to saline controls. Increased parabronchial lymph node (PLN) cell counts in WT mice, and restimulation of PLN cells with 20 μg/mL D. farinae induced release of IL-4, IL-5, and IL-13. Significantly elevated serum levels of D. farinae-specific IgE, D. farinae-specific IgG1, and total IgE in WT mice. Resulted in total BAL fluid cell numbers equivalent to saline controls in Pla2g5-null mice, with significantly reduced total BAL cells and eosinophils compared to D. farinae-treated WT mice. Showed 8 BVBs with cellular infiltrates per 15 BVBs, and 10 mucus-producing goblet cells per mm of bronchial basal lamina in Pla2g5-null mice. Significantly lowered lung mRNA expression of IL-5, IL-13, Muc5ac, and Clca3/Gob-5 in Pla2g5-null mice compared to D. farinae-treated WT mice. Significantly reduced PLN cell counts in Pla2g5-null mice compared to D. farinae-treated WT mice, and restimulated PLN cells released significantly lower levels of IL-4, IL-5, and IL-13. Dramatically lowered serum levels of D. farinae-specific IgE, D. farinae-specific IgG1, and total IgE in Pla2g5-null mice compared to D. farinae-treated WT mice.

Appearance

Solid

Color

Light brown to brown

SMILES

[House Dust Mite Extract, from D.farinae]

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Data Sheet (272 KB) SDS (251 KB)

COA (230 KB)

Handling Instructions (2659 KB)

References

[1]. Kim EM, et al. The storage mite Tyrophagus putrescentiae induces greater lung inflammation than house dust mites in mice. Parasites Hosts Dis. 2024;62(3):365-377. [Content Brief]

[2]. Giannattasio G, et al. Group V secretory phospholipase A2 reveals its role in house dust mite-induced allergic pulmonary inflammation by regulation of dendritic cell function. J Immunol. 2010;185(7):4430-4438. [Content Brief]