ICG-carboxylic acid

Name: ICG-carboxylic acid
Brand: MedChemExpress
SKU: HY-W088089
Rating: 4.5 (0 reviews)

Cat. No.: HY-W088089 Purity: 99.85%: Data Sheet
COA

SDS
Handling Instructions
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ICG-carboxylic acid is near-infrared (NIR) fluorescent probe. ICG is a fluorescent dye used in medical diagnostics. ICG has absorption peaking at 800 nm and can absorb the near IR laser energy and release heat in the dyed tissue (Ex/Em = 785/810 nm).

For research use only. We do not sell to patients.

ICG-carboxylic acid Chemical Structure

CAS No. : 181934-09-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Other Forms of ICG-carboxylic acid:

ICG acid Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Guidelines (The following is our recommended protocol; this protocol serves merely as a guideline and should be modified according to your specific needs)^[3]
1. Add 1 mg of ICG-carboxylic acid to 10 mL of water containing 100 mg of the aluminum-rich polysaccharide “SpaTA”; subsequently, mix with 50 mg of EDC/NHS and allow the reaction to proceed catalytically at room temperature for 6 hours.
2. Transfer the solution into a micro-dialysis bag, then purify it using 10 mM PBS.
3. Replace the dialysis buffer every 4 hours, for a total of three changes.
4. Determine the Relative Fluorescence Units (RFU) of the ICG-loaded SpaTA and the ICG standard using fluorescence spectrophotometry, with an excitation wavelength of 805 nm and an emission wavelength of 835 nm.
5. The formula for calculating the ICG Loading Rate (LR) is as follows:
LR% = RFU_Sample / RFU_Standard.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

730.95

Formula

C₄₅H₅₀N₂O₅S

CAS No.

181934-09-8

Appearance

Solid

Color

Brown to black

Emission (Em)

810

Excitation (Ex)

785

SMILES

CC1(C)C(/C=C/C=C/C=C/C=C2C(C)(C)C(C(C=CC=C3)=C3C=C4)=C4N\2CCCCCC([O-])=O)=[N+](CCCCS(=O)(O)=O)C5=C1C6=CC=CC=C6C=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (68.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3681 mL	6.8404 mL	13.6808 mL
5 mM	0.2736 mL	1.3681 mL	2.7362 mL
10 mM	0.1368 mL	0.6840 mL	1.3681 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (258 KB) HNMR (296 KB) RP-HPLC (219 KB)

Handling Instructions (2659 KB)

References

[1]. Taylor JS, et al. Combined application of Indocyanine green (ICG) and laser lead to targeted tumor cell destruction. J Pediatr Surg. 2018;53(12):2475-2479. [Content Brief]

[2]. Mačianskienė R, et al. Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart. Sci Rep. 2017;7(1):7983. Published 2017 Aug 11. [Content Brief]

[3]. Wu YZ, et al. From Ancient Medicine to Targeted Nanocarrier: A Sparganii Rhizoma-Derived Nanoparticle for Diagnostic Imaging and Endocrine Therapy in Cancer. ACS Appl Bio Mater. 2020 Apr 20;3(4):2028-2039. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3681 mL	6.8404 mL	13.6808 mL	34.2021 mL
	5 mM	0.2736 mL	1.3681 mL	2.7362 mL	6.8404 mL
	10 mM	0.1368 mL	0.6840 mL	1.3681 mL	3.4202 mL
	15 mM	0.0912 mL	0.4560 mL	0.9121 mL	2.2801 mL
	20 mM	0.0684 mL	0.3420 mL	0.6840 mL	1.7101 mL
	25 mM	0.0547 mL	0.2736 mL	0.5472 mL	1.3681 mL
	30 mM	0.0456 mL	0.2280 mL	0.4560 mL	1.1401 mL
	40 mM	0.0342 mL	0.1710 mL	0.3420 mL	0.8551 mL
	50 mM	0.0274 mL	0.1368 mL	0.2736 mL	0.6840 mL
	60 mM	0.0228 mL	0.1140 mL	0.2280 mL	0.5700 mL