2. MCE-Kits
  3. Molecular Biology
  4. PCR & qPCR
  5. Polymerase Chain Reaction (PCR)
  6. 2× PCR Master Mix (with Dye)

2× PCR Master Mix (with Dye) 

Art. -Nr.: HY-K0531
Manual COA SDS Technical Support

MCE 2× PCR Master Mix (with Dye) is provided as a simple-to-use, stabilized 2× formulation that includes all components for PCR except sample DNA, primers and water, in which bromophenol blue dye is included. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

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2 Publications Citing Use of MCE 2× PCR Master Mix (with Dye)

  • Beschreibung

  • Speicherung

  • Komponenten

  • Dokumentation

Description
& Advantages

MCE 2× PCR Master Mix (with Dye) is a convenient, ready-to-use kit for PCR. The 2× PCR Master Mix contains all the reagents necessary for routine PCR, including Taq DNA Polymerase, dNTPs Mix, MgCl2 and optimized reaction buffer. With the primers and template added, the optimized system will provide sensitive and reliable DNA synthesis. After the PCR reaction, the PCR products can be loaded directly onto an agarose gel. It saves preparation time, reduces the risk of contamination from multiple pipetting steps, and provides consistent reaction-to-reaction performance. The obtained PCR products contain poly(A) at the 3'-end and can be directly cloned into T-Vectors.
Speicherung

Store at -20°C for 2 years. 4°C for short-term storage (up to 3 months). Avoid repetitive freeze-thaw cycles while using.
Komponenten
Components HY-K0531- 1 mL HY-K0531- 5 mL HY-K0531- 20 mL
2× PCR Master Mix (with Dye) 1 mL 1 mL × 5 (1 mL × 5) × 4
Dokumentation

Manual (175 KB) SDS (102 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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2× PCR Master Mix (with Dye)
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