2. MCE-Kits
  3. Cell Biology
  4. Cell Analysis
  5. Metabolism Assay Kit
  6. Caspase 1 Activity Assay Kit

Caspase 1 Activity Assay Kit 

Art. -Nr.: HY-K2610
Manual COA SDS Technical Support

MCE Caspase 1 Activity Assay Kit provides a simple and convenient way for detecting the activity of detection of Caspase 1 activity that based on the spectrophotometric.

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1 Publications Citing Use of MCE Caspase 1 Activity Assay Kit

  • Beschreibung

  • Speicherung

  • Komponenten

  • Dokumentation

Description
& Advantages

Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-1 (Interleukin 1β converting enzyme (ICE)) is an interleukin-1 converting enzyme that proteolytically cleaves the precursor protein of the inflammatory cytokines interleukin 18 and interleukin 1β into active forms, regulates apoptosis by shearing Bcl-XL and plays a key role in regulating host responses to inflammatory stimuli through its action on various cytokines.

MCE Caspase 1 Activity Assay Kit provides a simple and convenient way for detecting the activity of detection of Caspase 1 activity that based on the spectrophotometric. Caspase 1 catalyzes the substrate Ac-YVAD-pNA and produces yellow pNA, and the luminescence of the pNA is quantified using a spectrophotometer at 405 nm to determine the activity of Caspase 1.
Speicherung

-20°C, 1 year

Keep away from light
Komponenten
Components HY-K2610-100T
Lysis Buffer 20 mL
Assay Buffer 10 mL
Ac-YVAD-pNA (2 mM) 1 mL
pNA (10 mM) 1 mL
Dokumentation

Manual (2872 KB) SDS (251 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Apoptosis kitapoptosis detectionCaspase1 activity assay kitapoptosis staining

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Caspase 1 Activity Assay Kit
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HY-K2610
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