2. Apoptosis
  3. Apoptosis
  4. Kaempferol 3-O-β-D-galactopyranoside

Kaempferol 3-O-β-D-galactopyranoside  (Synonyms: Trifolin)

製品番号: HY-N6605 純度: 99.58%
COA 取扱説明書 Technical Support

Kaempferol 3-O-β-D-galactopyranoside (Trifolin) is an orally active flavonoid derivative that can be found in the aerial parts of Consolida oliverana. Kaempferol 3-O-β-D-galactopyranoside exhibits antitumor and cardioprotective effects.

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Kaempferol 3-O-β-D-galactopyranoside

Kaempferol 3-O-β-D-galactopyranoside 構造式

CAS 番号 : 23627-87-4

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 385 在庫あり
Solution
10 mM * 1 mL in DMSO USD 385 在庫あり
Solid
1 mg $160 在庫あり
5 mg $350 在庫あり
10 mg $550 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Kaempferol 3-O-β-D-galactopyranoside 関連抗体

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  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

Kaempferol 3-O-β-D-galactopyranoside (Trifolin) is an orally active flavonoid derivative that can be found in the aerial parts of Consolida oliverana. Kaempferol 3-O-β-D-galactopyranoside exhibits antitumor and cardioprotective effects[1][2][3].

Cellular Effect
Cell Line Type Value Description References
J774.2 IC50
161.32 μM
Compound: 3
Cytotoxicity against BALB/c mouse J774.2 cells after 72 hrs by trypan blue assay
Cytotoxicity against BALB/c mouse J774.2 cells after 72 hrs by trypan blue assay
[PMID: 19489596]
MDCK CC50
143.3 μg/mL
Compound: 10
Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
[PMID: 15104496]
MDCK CC50
215 μg/mL
Compound: 10
Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
[PMID: 15104496]
MDCK IC50
>300 μg/mL
Compound: 10
Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
[PMID: 15104496]
MDCK IC50
143.3 μg/mL
Compound: 10
Cytotoxicity against MDCK cells
Cytotoxicity against MDCK cells
[PMID: 15104496]
MDCK IC50
53.8 μg/mL
Compound: 10
Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
[PMID: 15104496]
MDCK IC50
71.7 μg/mL
Compound: 10
Antiviral activity against RSV Long in MDCK cells assessed as inhibition of virus-induced cytopathic effect
Antiviral activity against RSV Long in MDCK cells assessed as inhibition of virus-induced cytopathic effect
[PMID: 15104496]
体外実験

Kaempferol 3-O-β-D-galactopyranoside (12.5–50 μM, 24-48 h) inhibits the proliferation of NCI-H460 cells in a dose-dependent manner[2].
Kaempferol 3-O-β-D-galactopyranoside (12.5–50 μM, 48 h) increases the Sub-G1 phase cell population and induces apoptosis in NCI-H460 cells[2].
Kaempferol 3-O-β-D-galactopyranoside (12.5–50 μM, 48 h) modulates intrinsic and extrinsic apoptosis signaling pathways and induces apoptosis in NCI-H460 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Kaempferol 3-O-β-D-galactopyranoside 関連抗体

Cell Viability Assay[2]

Cell Line: NCI-H460 human non-small cell lung cancer cells
Concentration: 12.5–50 μM
Incubation Time: 24 h and 48 h
Result: Decreased cell viability to less than 50% at 50 μM after 48 h;
Showed significant cytotoxicity only at 50 μM after 24 h.

Cell Cycle Analysis[2]

Cell Line: NCI-H460 human non-small cell lung cancer cells
Concentration: 12.5–50 μM
Incubation Time: 48 h
Result: Increased Sub-G1 phase cell population, decreased G1 phase cell population, no significant change in G2/M phase.

Western Blot Analysis[2]

Cell Line: NCI-H460 human non-small cell lung cancer cells
Concentration: 12.5–50 μM
Incubation Time: 24 h and 48 h
Result: Increased expression of Fas, FADD, and Bax;
Decreased expression of Bcl-2 and cIAP-1.
体内実験

Kaempferol 3-O-β-D-galactopyranoside (0.1–10.0 mg/kg, p.o., once daily for 4 weeks) improves cardiac function and reduces cardiomyocyte apoptosis in Angiotensin II (AngII) (HY-13948)-induced hypertensive cardiac injury C57BL/6 mice model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Angiotensin II (AngII) (HY-13948)-induced hypertensive cardiac injury C57BL/6 mice model[3]
Dosage: 0.1, 1.0, and 10.0 mg/kg
Administration: Oral gavage (p.o.), once daily for 4 weeks
Result: Improved left ventricular ejection fraction (LVEF) and fractional shortening (LVFS).
Alleviated inflammatory cell infiltration and pathological damage in cardiac tissue
. Reduced cardiomyocyte apoptosis and regulated Bax, Bcl-2, and cleaved caspase-3 protein expression
分子量

448.38

分子式

C21H20O11
CAS 番号
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1C2=C(O)C=C(O)C=C2OC(C3=CC=C(O)C=C3)=C1O[C@@H]4O[C@@H]([C@H](O)[C@H](O)[C@H]4O)CO
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : 25 mg/mL (55.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2303 mL 11.1513 mL 22.3025 mL
5 mM 0.4461 mL 2.2303 mL 4.4605 mL
10 mM 0.2230 mL 1.1151 mL 2.2303 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

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体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (2.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.58%

COA (259 KB) HNMR (271 KB) LCMS (94 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2303 mL 11.1513 mL 22.3025 mL 55.7563 mL
5 mM 0.4461 mL 2.2303 mL 4.4605 mL 11.1513 mL
10 mM 0.2230 mL 1.1151 mL 2.2303 mL 5.5756 mL
15 mM 0.1487 mL 0.7434 mL 1.4868 mL 3.7171 mL
20 mM 0.1115 mL 0.5576 mL 1.1151 mL 2.7878 mL
25 mM 0.0892 mL 0.4461 mL 0.8921 mL 2.2303 mL
30 mM 0.0743 mL 0.3717 mL 0.7434 mL 1.8585 mL
40 mM 0.0558 mL 0.2788 mL 0.5576 mL 1.3939 mL
50 mM 0.0446 mL 0.2230 mL 0.4461 mL 1.1151 mL
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Kaempferol 3-O-β-D-galactopyranoside Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Kaempferol 3-O-β-D-galactopyranoside23627-87-4TrifolinApoptosisHypertensive cardiac injury mice modelNCI-H460 cellsInhibitorinhibitorinhibit

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