2. Apoptosis Cell Cycle/DNA Damage
  3. Apoptosis DNA/RNA Synthesis
  4. L-Asparaginase

L-Asparaginase (L-ASNase) is a deamidating enzyme that catalyses the hydrolysis of L-asparagine and L-glutamine, and can be used for the research of acute lymphoblastic leukemia. L-Asparaginase depletes L-asparagine from plasma resulting in inhibition of RNA and DNA synthesis with the subsequent blastic cell apoptosis.

For research use only. We do not sell to patients.

CAS No. : 9015-68-3

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Based on 15 publication(s) in Google Scholar

L-Asparaginase Related Antibodies

Top Publications Citing Use of Products

    L-Asparaginase purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Jun 20;11(25):eadt3075.  [Abstract]

    Transwell migration assay of indicated cells in the presence of ʟ-Asn(50 μg/ml) or ʟ-ASNase (L-Asparaginase; 10 μg/mL; 24-36 h).

    L-Asparaginase purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Jun 20;11(25):eadt3075.  [Abstract]

    Brain slices of mice were stained with hematoxylin andeosin (H&E) at the endpoint. BCBM model mice, either cisplatin (4 mg/kg, intraperitoneally) or ʟ-ASNase (L-Asparaginase; 0.2 mg per mouse, intraperitoneally) was administered weekly or every 2 days for 2 weeks, respectively.

    L-Asparaginase purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Jun 20;11(25):eadt3075.  [Abstract]

    BLI was conducted at regular intervals. Representative BLI images and line chart of radiance. BCBM model, Mice were administered cisplatin (4 mg/kg, intraperitoneally) weekly or ʟ-ASNase (0.2 mg per mouse, intraperitoneally) every 2 days.

    L-Asparaginase purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Aug 1;14(8):489.  [Abstract]

    MNNG/HOS cells with or without NUCKS1 depletion were treated with 10 μM L-Asparaginase for 48 h. Cell death was measured by PI staining.

    L-Asparaginase purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Aug 1;14(8):489.  [Abstract]

    MNNG/HOS cells with or without NUCKS1 depletion were treated with 10 μM L-Asparaginase for 48 h. Cell death was measured by PI staining.

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    Description

    L-Asparaginase (L-ASNase) is a deamidating enzyme that catalyses the hydrolysis of L-asparagine and L-glutamine, and can be used for the research of acute lymphoblastic leukemia. L-Asparaginase depletes L-asparagine from plasma resulting in inhibition of RNA and DNA synthesis with the subsequent blastic cell apoptosis[1].

    In Vitro

    L-Asparaginase has a cytotoxic effect on AML blasts and LSC subpopulations[1].
    L-Asparaginase is effective on both CD34+CD38+ and CD34+CD38+ LSC fractions, MSC and monocytes/macrophages partially counteracted the effect[1].
    Although lyophilized L-asparaginase is stable at room temperature for 3 weeks, it should be stored dry below -18°C. L-Asparaginase should Co-culture with cells at 37°C, 5% CO2 for 24, 48 h.
    L-Asparaginase (100 IU/mL, 0-24 h) is co-cultured with L5178Y and B16 cells (5×105/mL), and cells are arrested in the G1 phase and DNA strand breaks occurred[2].
    Product Information
    Optimal pH: 8.0-9.0
    Agonists: Anaerobic conditions, amino acids
    Inhibitors: 5-diazo-4-o-L-norvaline (DONV), dimethyl sulfoxide, glutaraldehyde, L-asparagine and L-aspartic acid, NH4+, trypsin

    Instructions
    Dissolve in 1 mg/ml 0.2 M Tris-HCl pH 8.6, store at 2-8°C after dissolution, and use as soon as possible within 1 week. Or dissolve according to specific experimental references.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Asparaginase Related Antibodies
    Clinical Trial
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    [L-Asparaginase]
    EC Number

    3.5.1.1
    Molecular Weight

    34000 (monomer)

    Specific Activity

    100 - 300 U/mg protein
    Unit Definition

    One unit will release 1.0μmol of ammonia from L-asparagine per minute at pH8.5 and 37°C.
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (Need ultrasonic)

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 25 mg/mL; Clear solution; Need ultrasonic

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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    SDS (418 KB)

    COA (231 KB)

    Handling Instructions (2659 KB)
    References
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    L-Asparaginase Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Asparaginase9015-68-3L-​ASNaseApoptosisDNA/RNA Synthesisdeamidating enzymeL-asparagineacute lymphoblastic leukemiaRNA synthesisDNA synthesisapoptosiscancerInhibitorinhibitorinhibit

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