LYT-200 

LYT-200 is a humanized monoclonal antibody against galectin-9 (Galectin-9). LYT-200 binds to galectin-9 expressed on the surface of hematologic malignant cells, inhibits pro-leukemic functions and induces cell death. LYT-200 can be used in research related to hematologic malignancies^[1].

Human IgG4 kappa

Human IgG4 (S228P) kappa, Isotype Control

Human

LYT-200 (10-100 ng/mL; 48 or 72 h) induces extensive cell death in human B-ALL, AML, T-ALL, and DLBCL cell lines^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LYT-200 (1.5 mg/kg; weekly) administered at 1.5 mg/kg weekly provides survival benefit in immunocompromised mice with T-ALL comparable to methotrexate, and combination treatment with methotrexate yields the best survival outcomes in these models^[1].
LYT-200 (1.5 mg/kg; weekly) administered at 1.5 mg/kg weekly provides superior survival protection compared to cytarabine in immunocompromised mice with AML, and combination treatment with cytarabine yields greater than 80% survival at 2 months post-disease appearance^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3965  [NCBI]

O00182

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

145.46 kDa

Liquid

Colorless to light yellow

[LYT-200]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow cytometric analysis of 1×10⁶ Jurkat cells labelling Galectin-9 (red) with LYT-200 (HY-P991228). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 kappa Isotype Control (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
• 
  Immobilized Galectin-9/LGALS9 Protein, Human (HEK293, His) can bind LYT-200. The EC₅₀ for this effect is 40.78 ng/mL.

• [1]. Curtis J Henry, et al. Lyt-200, a Humanized Anti-Galectin-9 Antibody, Exhibits Preclinical Efficacy in Models of Hematological Malignancies. Blood (2022) 140 (Supplement 1): 8837.