1. Academic Validation
  2. Polysaccharide isolated from Sarcodon aspratus induces RAW264.7 activity via TLR4-mediated NF-κB and MAPK signaling pathways

Polysaccharide isolated from Sarcodon aspratus induces RAW264.7 activity via TLR4-mediated NF-κB and MAPK signaling pathways

  • Int J Biol Macromol. 2018 Dec;120(Pt A):1039-1047. doi: 10.1016/j.ijbiomac.2018.08.147.
Dan-Dan Wang 1 Wen-Juan Pan 2 Shomaila Mehmood 1 Xiao-Du Cheng 1 Yan Chen 3
Affiliations

Affiliations

  • 1 School of Life Sciences, Anhui University, Hefei 230601, China.
  • 2 School of Life Sciences, Anhui University, Hefei 230601, China. Electronic address: [email protected].
  • 3 School of Life Sciences, Anhui University, Hefei 230601, China; Anhui Key Laboratory of Modern Biomanufacturing, Hefei 230601, China. Electronic address: [email protected].
Abstract

Our previous report showed that the novel polysaccharide SAP isolated from the fruiting bodies of Sarcodon aspratus induced Hela cells Apoptosis via mitochondrial dysfunction. In this study we found that SAP enhanced immunostimulatory activities of RAW264.7 cells, which was characterized by increased the production of nitric oxide (NO), Reactive Oxygen Species (ROS), cytokines and phagocytic. However, SAP-induced macrophage activation was abolished when Toll-like Receptor 4 (TLR4) signaling was blocked by anti-TLR4 Antibodies. Moreover, according to the Western blot analysis and use of specific inhibitors against the MAPKs (mitogen-activated protein kinases) and NF-κB (nuclear factor-κB), we speculated that SAP activated RAW264.7 cells through TLR4-mediated activation of NF-κB and MAPKs pathways. Thus, Sarcodon aspratus is a potential immunomodulator that can be used as healthcare food.

Keywords

Polysaccharide; RAW264.7 macrophages; Sarcodon aspratus.

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