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  3. RNA-seq reveals correlations between cytoskeleton-related genes and the osteogenic activity of mesenchymal stem cells on strontium loaded titania nanotube arrays

RNA-seq reveals correlations between cytoskeleton-related genes and the osteogenic activity of mesenchymal stem cells on strontium loaded titania nanotube arrays

  • Mater Sci Eng C Mater Biol Appl. 2021 Mar:122:111939. doi: 10.1016/j.msec.2021.111939.
Tianxiao Han 1,  Peng Xue 2,  Shiying Ju 1,  Zutai Zhang 3,  Jianyu Zeng 1,  Yumei Zhang 4,  Qingsong Jiang 5
Affiliations

Affiliations

  • 1 Department of Prosthodontics, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing 100050, China.
  • 2 Institute of Stomatology, Chinese PLA General Hospital, Beijing 100853, China.
  • 3 Beijing Institute of Dental Research, Capital Medical University, School of Stomatology, Beijing 100050, China.
  • 4 State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China. Electronic address: [email protected].
  • 5 Department of Prosthodontics, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing 100050, China. Electronic address: [email protected].
PMID: 33641927 DOI: 10.1016/j.msec.2021.111939
Abstract

Strontium loaded titania nanotube arrays (NTSr), as well as titania nanotube arrays (NT), have been regarded as effective coatings to promote bone regeneration on titanium implants, but an understanding of the full extent of early processes affected by such surface modifications is absent. To address this limitation, we performed RNA Sequencing (RNA-seq) of Sprague-Dawley rat bone marrow mesenchymal stem cells (rBMMSCs) cultured on unmodified titanium sheets (Con), NT and NTSr specimens. By pairwise comparisons we found that NT and NTSr shared a majority of differentially expressed genes. The Gene Ontology (GO) analysis revealed that NT and NTSr up-regulated a bunch of genes that are annotated to the Cytoskeleton. The results were supported by immunofluorescent, transmission electron microscopy (TEM) and western blotting assays. By inhibiting the Cytoskeleton through pharmacological agents, the activities of Alkaline Phosphatase (ALP) on NT and NTSr were also suppressed. Informed by these results, we concluded that NT and NTSr specimens reorganized the Cytoskeleton of cultured cells that may play a crucial role in osteogenic lineage commitment.

Keywords

Cytoskeleton; Osteogenesis; RNA-seq; Strontium; Titania nanotube.

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