Art. -Nr.: HY-120145

MST-312

(Synonyms: Telomerase Inhibitor IX)

2 Cited Publications

Kundenbewertung

Based on 2 publication(s) in Google Scholar

MST-312 is a telomerase inhibitor. MST-312 is a chemically modified derivative of green tea epigallocatechin gallate (EGCG). MST-312 can be used for the research of cancer, such as multiple myeloma (MM).

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Reinheit: 98.62%
CAS. Nr.: 368449-04-1
Formel: C₂₀H₁₆N₂O₆
Molecular Weight:380.35

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

MST-312 Chemical Structure

CAS. Nr.: 368449-04-1

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

telomeras^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	12.1 μM Compound: 5, MST-312	Inhibition of telomerase in human HeLa cells using 5'-AAT CCG TCG AGC AGA GTT-3' as substrate incubated for 15 mins prior to extension reaction by telomeric repeat amplification protocol Inhibition of telomerase in human HeLa cells using 5'-AAT CCG TCG AGC AGA GTT-3' as substrate incubated for 15 mins prior to extension reaction by telomeric repeat amplification protocol	[PMID: 22413845]
HeLa	IC₅₀	12.8 μM Compound: 5, MST-312	Inhibition of telomerase in human HeLa cells using 5'-AAT CCG TCG AGC AGA GTT-3' as substrate incubated for 15 mins prior to extension reaction followed by compound washout by spin-telomeric repeat amplification protocol Inhibition of telomerase in human HeLa cells using 5'-AAT CCG TCG AGC AGA GTT-3' as substrate incubated for 15 mins prior to extension reaction followed by compound washout by spin-telomeric repeat amplification protocol	[PMID: 22413845]
U-937	IC₅₀	0.67 μM Compound: 5, MST-312	Inhibition of telomerase in human U937 cells by telomeric repeat amplification protocol Inhibition of telomerase in human U937 cells by telomeric repeat amplification protocol	[PMID: 22413845]
U-937	IC₅₀	0.67 μM Compound: MST-312	Inhibition of human telomerase isolated from human U937 cellular extracts by SYBR Green staining-based TRAP assay Inhibition of human telomerase isolated from human U937 cellular extracts by SYBR Green staining-based TRAP assay	[PMID: 24053596]
U-937	IC₅₀	0.67 μM Compound: MST-312	Inhibition of telomerase extracted from human U937 cells by TRAP assay Inhibition of telomerase extracted from human U937 cells by TRAP assay	10.1039/C0MD00241K
U-937	IC₅₀	0.67 μM Compound: 24	Antiproliferative activity against human U-937 cells measured upto 72 hrs Antiproliferative activity against human U-937 cells measured upto 72 hrs	[PMID: 37776575]
U-937	IC₅₀	1.7 μM Compound: 24	Antiproliferative activity against human U-937 cells assessed as cell growth inhibition measured after 72 hrs Antiproliferative activity against human U-937 cells assessed as cell growth inhibition measured after 72 hrs	[PMID: 37776575]

In Vitro

MST-312 (2~8 μM; 0~72 hours; U-266 cells) reduces cellular viability in a dose dependent and time-dependent manner^[1].
MST-312 (2~8 μM; 48 hours; U-266 cells) induces cell apoptosis in a dose-dependent manner^[1].
MST-312 (2 μM; 48 hours; U-266 cells) up-regulates the pro-apoptotic gene Bax and down-regulates the anti-apoptotic gene Bcl-2 and suppresses the expression of c-Myc and hTERT genes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

U-266 cells
Concentration:

2~8 μM
Incubation Time:

0~72 hours
Result:

The viability of U-266 cells was substantially decreased in a dose dependent and time-dependent manner, in response to exposure to MST-312.

Apoptosis Analysis^[1]

Cell Line:

U-266 cells
Concentration:

2~8 μM
Incubation Time:

48 hours
Result:

Induced cell apoptosis in a dose-dependent manner.

RT-PCR^[1]

Cell Line:

U-266 cells
Concentration:

2 μM
Incubation Time:

48 hours
Result:

Up-regulated the pro-apoptotic gene Bax and down-regulated the anti-apoptotic gene Bcl-2 and suppressed the expression of c-Myc and hTERT genes.

Chemical Information

CAS. Nr. 368449-04-1
Appearance Solid
Molecular Weight 380.35
Formel C₂₀H₁₆N₂O₆
Color White to off-white
SMILES

O=C(NC1=CC=CC(NC(C2=CC=CC(O)=C2O)=O)=C1)C3=CC=CC(O)=C3O
Synonyms

Telomerase Inhibitor IX
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Journal Impact Factor

J Exp Med

Inhibition of hTERT/telomerase/telomere mediates therapeutic efficacy of osimertinib in EGFR mutant lung cancer. [Abstract]

2024 Nov 4;221(11):e20240435. PMID: 39297884
Biomed Pharmacother

Azvudine exhibits potent differentiation-inducing effect by targeting the TERT/p21 axis in acute myeloid leukemia. [Abstract]

2025 May 27:188:118215. PMID: 40435838

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (262.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6292 mL

13.1458 mL

26.2916 mL

5 mM

0.5258 mL

2.6292 mL

5.2583 mL

10 mM

0.2629 mL

1.3146 mL

2.6292 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (458 KB)
- English - EN (458 KB)
- Français - FR (458 KB)
- Deutsch - DE (458 KB)
- Norwegian - NO (458 KB)
- Español - ES (458 KB)
- Swedish - SV (458 KB)
- Italian - IT (458 KB)
- Korean - KR (458 KB)
- Portuguese - PT (458 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Ameri Z, et al. Telomerase inhibitor MST-312 induces apoptosis of multiple myeloma cells and down-regulation of anti-apoptotic, proliferative and inflammatory genes. Life Sci. 2019;228:66-71. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6292 mL	13.1458 mL	26.2916 mL	65.7289 mL
	5 mM	0.5258 mL	2.6292 mL	5.2583 mL	13.1458 mL
	10 mM	0.2629 mL	1.3146 mL	2.6292 mL	6.5729 mL
	15 mM	0.1753 mL	0.8764 mL	1.7528 mL	4.3819 mL
	20 mM	0.1315 mL	0.6573 mL	1.3146 mL	3.2864 mL
	25 mM	0.1052 mL	0.5258 mL	1.0517 mL	2.6292 mL
	30 mM	0.0876 mL	0.4382 mL	0.8764 mL	2.1910 mL
	40 mM	0.0657 mL	0.3286 mL	0.6573 mL	1.6432 mL
	50 mM	0.0526 mL	0.2629 mL	0.5258 mL	1.3146 mL
	60 mM	0.0438 mL	0.2191 mL	0.4382 mL	1.0955 mL
	80 mM	0.0329 mL	0.1643 mL	0.3286 mL	0.8216 mL
	100 mM	0.0263 mL	0.1315 mL	0.2629 mL	0.6573 mL

Keywords

MST-312

2 Cited Publications

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MST-312

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Anti-Blood Cancer Compound Library

Targeted Diversity Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) MST-312

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

MST-312

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Anti-Blood Cancer Compound Library

Targeted Diversity Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) MST-312

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

MST-312 Related Classifications

HY-120145 Related Classifications