Pancreatic lipase/Carboxylesterase 1-IN-1

Name: Pancreatic lipase/Carboxylesterase 1-IN-1
Brand: MedChemExpress
SKU: HY-144809
Rating: 4.5 (0 reviews)

Art. -Nr.: HY-144809 Reinheit: 99.92%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Pancreatic lipase/Carboxylesterase 1-IN-1 (Compound 39) is a potent dual inhibitor of pancreatic lipase (PL) and human carboxylesterase 1A (hCES1A) with IC₅₀ values of 2.13 µM and 0.055 µM against PL and hCES1A.

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Pancreatic lipase/Carboxylesterase 1-IN-1 Chemische Struktur

CAS. Nr. : 194235-21-7

Größe	Verfügbarkeit	Menge
5 mg	Auf Lager	Estimated Time of Arrival: December 31
10 mg	Auf Lager	Estimated Time of Arrival: December 31
50 mg	Angebot einholen
100 mg	Angebot einholen

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Kundenbewertung

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Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle Carboxylesterase (CES) Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

CES1 CES2 CES3

Biologische Aktivität
Reinheit & Dokumentation
Verweise
Kundenbewertung

Beschreibung

IC₅₀ & Target

IC₅₀: 0.055 µM (hCES1A), 2.13 µM (PL), 6.02 µM (hCES2A)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Macrophage	IC₅₀	37 3 Compound: 17	Antiinflammatory activity in mouse macrophages assessed as inhibition of IFN-gamma-induced nitric oxide production after 48 hrs by Griess method Antiinflammatory activity in mouse macrophages assessed as inhibition of IFN-gamma-induced nitric oxide production after 48 hrs by Griess method	[PMID: 23837912]
Macrophage	IC₅₀	37 3 Compound: 17	Antiinflammatory activity in mouse macrophages assessed as inhibition of IFN-gamma-induced nitric oxide production after 48 hrs by Griess method Antiinflammatory activity in mouse macrophages assessed as inhibition of IFN-gamma-induced nitric oxide production after 48 hrs by Griess method	[PMID: 23837912]
Macrophage	IC₅₀	37 3 Compound: 17	Antiinflammatory activity in mouse macrophages assessed as inhibition of IFN-gamma-induced nitric oxide production after 48 hrs by Griess method Antiinflammatory activity in mouse macrophages assessed as inhibition of IFN-gamma-induced nitric oxide production after 48 hrs by Griess method	[PMID: 23837912]

Molekulargewicht

468.67

Formel

C₃₀H₄₄O₄

CAS. Nr.

194235-21-7

Appearance

Solid

Color

White to off-white

SMILES

[H][C@@]12CC(C)(CC[C@@]1(CC[C@@]3(C2=CC[C@@]4([C@]5(C=C(C(C(C)([C@]5([H])CC[C@@]34C)C)=O)O)C)[H])C)C(O)=O)C

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (213.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1337 mL	10.6685 mL	21.3370 mL
5 mM	0.4267 mL	2.1337 mL	4.2674 mL
10 mM	0.2134 mL	1.0668 mL	2.1337 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.33 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.33 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (5.33 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Reinheit & Dokumentation

Purity: 99.92%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (252 KB) HNMR (294 KB) RP-HPLC (211 KB) LCMS (284 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Zhang J, et al. Discovery of triterpenoids as potent dual inhibitors of pancreatic lipase and human carboxylesterase 1. J Enzyme Inhib Med Chem. 2022 Dec;37(1):629-640. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1337 mL	10.6685 mL	21.3370 mL	53.3424 mL
	5 mM	0.4267 mL	2.1337 mL	4.2674 mL	10.6685 mL
	10 mM	0.2134 mL	1.0668 mL	2.1337 mL	5.3342 mL
	15 mM	0.1422 mL	0.7112 mL	1.4225 mL	3.5562 mL
	20 mM	0.1067 mL	0.5334 mL	1.0668 mL	2.6671 mL
	25 mM	0.0853 mL	0.4267 mL	0.8535 mL	2.1337 mL
	30 mM	0.0711 mL	0.3556 mL	0.7112 mL	1.7781 mL
	40 mM	0.0533 mL	0.2667 mL	0.5334 mL	1.3336 mL
	50 mM	0.0427 mL	0.2134 mL	0.4267 mL	1.0668 mL
	60 mM	0.0356 mL	0.1778 mL	0.3556 mL	0.8890 mL
	80 mM	0.0267 mL	0.1334 mL	0.2667 mL	0.6668 mL
	100 mM	0.0213 mL	0.1067 mL	0.2134 mL	0.5334 mL