2. Apoptosis Epigenetics
  3. Apoptosis SF3B1
  4. Pladienolide B

Pladienolide B is a potent cancer cell growth inhibitor that targets the SF3B1 subunit of the spliceosome. Pladienolide B exerts antitumor activities mediated through the inhibition of pre-mRNA splicing. Pladienolide B induces apoptosis.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 445493-23-2

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 2267 在庫あり
Solution
10 mM * 1 mL in DMSO USD 2267 在庫あり
Solid
100 μg $150 在庫あり
500 μg $480 在庫あり
1 mg $768 在庫あり
5 mg $1920 在庫あり
10 mg   お問い合わせ  
50 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 5 publication(s) in Google Scholar

Pladienolide B 関連抗体

Top Publications Citing Use of Products

    Pladienolide B purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 12;16(1):9946.  [Abstract]

    The splicing inhibitor Pladienolide B (500 nM, 3 h) reduced the expression of pro-inflammatory cytokines in control and YTHDF2 knockdown cells, and also reduced the level of U6 snRNA.

    Pladienolide B purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2025 Oct 17.  [Abstract]

    A375 and HCT-116 cells were treated with increasing concentrations of Pladienolide B (0-20 nM) and TG003 for 24 h and the levels of p21C and p21L were assessed with immunoblotting using 12D1 antibody; β-actin served as a loading control. OTS964 (200 nm) was used as a positive control. n = 3 biologically independent replicates.

    Pladienolide B purchased from MedChemExpress. Usage Cited in: J Proteomics. 2026 Jan 20:323:105565.  [Abstract]

    A protocol for quantitative proteomics analysis of SH-SY5Y cells treated with praldinolactone B (Pla-B) based on isotope labeling was developed to determine the dose-related effects of Pla-B on the cellular proteome. Cells were treated for 24 hours with increasing concentrations of Pla-B (1 pM to 10 nM) or DMSO (as a control).

    Pladienolide B purchased from MedChemExpress. Usage Cited in: J Proteomics. 2026 Jan 20:323:105565.  [Abstract]

    The heatmap shows the changes in protein expression under different concentrations of Pla-B (1 pM to 10 nM) treatment.

    Pladienolide B purchased from MedChemExpress. Usage Cited in: J Proteomics. 2026 Jan 20:323:105565.  [Abstract]

    The differential protein statistics table shows the number of proteins with significant changes in Pla-B concentration from 1 pM to 10 nM.

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Pladienolide B is a potent cancer cell growth inhibitor that targets the SF3B1 subunit of the spliceosome. Pladienolide B exerts antitumor activities mediated through the inhibition of pre-mRNA splicing. Pladienolide B induces apoptosis[1][2][3].

    体外実験

    Pladienolide B (0.1-2 nM; 24-72 hours) inhibits human cervical carcinoma cells viability[3].
    Pladienolide B (0.1-2 nM; 24-48 hours) reduces SF3b1 expression in human cervical carcinoma cells[3].
    Pladienolide B induces (0.1-2 nM; 24 hours) cell cycle arrest and apoptosis[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Pladienolide B 関連抗体

    Cell Viability Assay[3]

    Cell Line: HeLa cells
    Concentration: 0.1, 0.5, 1, 1.5, 2 nM
    Incubation Time: 24, 48, 72 hours
    Result: Significantly decreased cell viability, and the decrease was concentration- and time-dependent.

    Apoptosis Analysis[3]

    Cell Line: HeLa cells
    Concentration: 0.1, 0.5, and 2 nM
    Incubation Time: 24 hours
    Result: The apoptotic cells were highly induced at 24 hours.

    RT-PCR[3]

    Cell Line: HeLa cells
    Concentration: 0.1, 0.5, and 2 nM
    Incubation Time: 24, 48 hours
    Result: Induced a time- and concentration-dependent decrease in cellular SF3b1 proteins.
    体内実験

    Pladienolide B (2.5-10 mg/kg; i.v.; daily for 5 days) has strong antitumor activities[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female or male BALB/c nu/nu mice (7 weeks of age) (PC-3, OVCAR-3, DU-145, WiDr, and HCT-116, BSY-1 xenografts)[4]
    Dosage: 2.5, 5, and 10 mg/kg
    Administration: I.v.; daily for 5 days
    Result: Showed strong growth inhibitory or regressive activities against these xenografts.
    分子量

    536.70

    分子式

    C30H48O8
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1O[C@H](/C(C)=C/C=C/[C@@H](C)C[C@H]2O[C@@H]2[C@H](C)[C@@H](O)CC)[C@@H](C)/C=C/[C@H](OC(C)=O)[C@](C)(O)CC[C@@H](O)C1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (186.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8632 mL 9.3162 mL 18.6324 mL
    5 mM 0.3726 mL 1.8632 mL 3.7265 mL
    10 mM 0.1863 mL 0.9316 mL 1.8632 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.66 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.66 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.20%

    COA (247 KB) RP-HPLC (250 KB) LCMS (90 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.8632 mL 9.3162 mL 18.6324 mL 46.5810 mL
    5 mM 0.3726 mL 1.8632 mL 3.7265 mL 9.3162 mL
    10 mM 0.1863 mL 0.9316 mL 1.8632 mL 4.6581 mL
    15 mM 0.1242 mL 0.6211 mL 1.2422 mL 3.1054 mL
    20 mM 0.0932 mL 0.4658 mL 0.9316 mL 2.3290 mL
    25 mM 0.0745 mL 0.3726 mL 0.7453 mL 1.8632 mL
    30 mM 0.0621 mL 0.3105 mL 0.6211 mL 1.5527 mL
    40 mM 0.0466 mL 0.2329 mL 0.4658 mL 1.1645 mL
    50 mM 0.0373 mL 0.1863 mL 0.3726 mL 0.9316 mL
    60 mM 0.0311 mL 0.1553 mL 0.3105 mL 0.7763 mL
    80 mM 0.0233 mL 0.1165 mL 0.2329 mL 0.5823 mL
    100 mM 0.0186 mL 0.0932 mL 0.1863 mL 0.4658 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Pladienolide B Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pladienolide B445493-23-2ApoptosisSF3B1Splicing Factor 3b Subunit 1macrolideantitumorpre-mRNAsplicingcervicalcarcinomaHeLacellsSF3b1cyclearrestInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Pladienolide B
    製品番号:
    HY-16399
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.