Protamine sulfate

Name: Protamine sulfate
Brand: MedChemExpress
SKU: HY-107911
Rating: 5.0 (3 reviews)

Cat. No.: HY-107911 Pureza: 99.99%: Ficha de datos
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Protamine sulfate, polycationic peptide and a antiheparin agent, could neutralize the anticoagulant action of heparin and enhances lipid-mediated gene transfer.

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Protamine sulfate Estructura química

No. CAS : 9009-65-8

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100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	En stock	Estimated Time of Arrival: December 31
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1 g	Obtener un presupuesto

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Revisión del cliente

Based on 3 publication(s) in Google Scholar

Other Forms of Protamine sulfate:

Salmine sulfate Obtener un presupuesto

3 Publications Citing Use of MCE Protamine sulfate

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Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

Protamine sulfate, polycationic peptide and a antiheparin agent, could neutralize the anticoagulant action of heparin and enhances lipid-mediated gene transfer^[1]^[2]^[3].

In Vitro

Protamine sulfate has an inhibitory effect on thrombin in the conversion of fibrinogen to fibrin, and that this inhibition is concentration dependent, partial, and reversible^[3].
Protamine sulfate is a 5-kDa cationic polypeptide derived from salmon sperm that can bind negatively charged unfractionated heparin (UFH). Protamine sulfate down-regulates thrombin generation by inhibiting factor V activation^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Protamine sulfate can be used in animal modeling to create cystitis models^[5].

Induction of cystitis model^[5]

Background

The presence and activity of protamine sulfate may trigger the activation and inflammatory response of the host immune system. These immune responses can lead to the infiltration of inflammatory cells and the production of inflammatory mediators, such as cytokines and interleukins, which can lead to cystitis.

Specific Modeling Methods

Rat: Sprague-Dawley • female &bull
Administration: 10 mg/mL irrigation in the urinary bladder • once

Note

(1) 10 mg/ml protamine sulfate was injected into the rat bladder, and 2 mg/ml LPS was injected 30 minutes later
(2) 5 days later, the experimental animals were put to death under anesthesia, and the bladder was removed for observation.

Modeling Indicators

Physiological indicators: The turbidity, urine volume and the number of red blood cells in the urine of rats were detected.
Molecular changes: The levels of biomarkers IL-6, TNF-α and NF-κB in bladder tissues were detected.
Histological analysis: Hematoxylin and eosin staining were used to detect the number of mast cells in the bladder of rats.

Correlated Product(s): LPS (HY-D1056)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ensayo clínico

No. CAS

9009-65-8

Appearance

Solid

Color

White to off-white

SMILES

[Protamine sulfate]

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

H₂O : ≥ 33.33 mg/mL

*"≥" means soluble, but saturation unknown.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 33.33 mg/mL; Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureza y Documentación

Purity: 99.99%

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Ficha de datos (274 KB) SDS (251 KB)

COA (241 KB) RP-HPLC (206 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Ahmed Kouta, et al. Protamine Sulfate Neutralization Profile of Various Dosages of Bovine, Ovine and Porcine UFHs and Their Depolymerized Derivatives in Non-Human Primates. Clin Appl Thromb Hemost. Jan-Dec 2021;27:10760296211005544. [Content Brief]

[2]. F L Sorgi, et al. Protamine sulfate enhances lipid-mediated gene transfer. Gene Ther. 1997 Sep;4(9):961-8. [Content Brief]

[3]. R J Cobel-Geard, et al. Interaction of protamine sulfate with thrombin. Am J Hematol. 1983 May;14(3):227-33. [Content Brief]

[4]. Fionnuala Ni Ainle, et al. Protamine sulfate down-regulates thrombin generation by inhibiting factor V activation. Blood. 2009 Aug 20;114(8):1658-65. [Content Brief]

[5]. Li J, et al. Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model. Stem Cell Res Ther. 2017 May 8;8(1):107. [Content Brief]