Pseudomonas quinolone signal (Synonyms: PQS)

Name: Pseudomonas quinolone signal
Brand: MedChemExpress
SKU: HY-128200
Rating: 5.0 (2 reviews)

Cat. No.: HY-128200 Purity: 99.84%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Pseudomonas quinolone signal (PQS) is a quorum-sensing signaling molecule produced by Pseudomonas aeruginosa. The synthesis of Pseudomonas quinolone signal relies on the las quorum-sensing system, and its biological activity is associated with the rhl system. Pseudomonas quinolone signal regulates the expression of the virulence gene lasB. Additionally, Pseudomonas quinolone signal plays roles in iron acquisition, cytotoxicity, outer-membrane vesicle biogenesis, and host immune modulation.

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Pseudomonas quinolone signal 화학구조

CAS No. : 108985-27-9

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

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고객리뷰

Based on 2 publication(s) in Google Scholar

Other Forms of Pseudomonas quinolone signal:

Pseudomonas quinolone signal-d₄ 견적 받기

2 Publications Citing Use of MCE Pseudomonas quinolone signal

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
순도&문서
References
고객리뷰

제품 설명

In Vitro

Pseudomonas quinolone signal (0-200 μM; 18 h) can induce the expression of the lasB-lacZ fusion gene and activate β-galactosidase activity in P. aeruginosa strain PAO-R1 (lasR⁻) (pTS400)^[1].
Pseudomonas quinolone signal (40 μM; 5-24 h) promotes the synthesis of pyochelin and pyocyanin, increases bacterial sensitivity to H₂O₂, and reduces iron concentration in the culture medium in P. aeruginosa PAO1^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

259.35

화학식

C₁₆H₂₁NO₂

CAS No.

108985-27-9

Appearance

Solid

Color

White to light brown

SMILES

O=C1C(O)=C(CCCCCCC)NC2=C1C=CC=C2

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

용액&용해도

In Vitro:

DMSO : 50 mg/mL (192.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8558 mL	19.2790 mL	38.5579 mL
5 mM	0.7712 mL	3.8558 mL	7.7116 mL
10 mM	0.3856 mL	1.9279 mL	3.8558 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.84%

Select Batch:

Data Sheet (272 KB) SDS (394 KB)

COA (244 KB) HNMR (265 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Pesci E C, et al. Quinolone signaling in the cell-to-cell communication system of Pseudomonas aeruginosa[J]. Proceedings of the National Academy of Sciences, 1999, 96(20): 11229-11234. [Content Brief]

[2]. Bredenbruch F, et al. The Pseudomonas aeruginosa quinolone signal (PQS) has an iron-chelating activity. Environ Microbiol. 2006 Aug;8(8):1318-29. [Content Brief]

[3]. Cooke AC, et al. Pseudomonas Quinolone Signal-Induced Outer Membrane Vesicles Enhance Biofilm Dispersion in Pseudomonas aeruginosa. mSphere. 2020 Nov 25;5(6):e01109-20. [Content Brief]

[4]. Lin J, et al. The Pseudomonas Quinolone Signal (PQS): Not Just for Quorum Sensing Anymore. Front Cell Infect Microbiol. 2018 Jul 4;8:230. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8558 mL	19.2790 mL	38.5579 mL	96.3948 mL
	5 mM	0.7712 mL	3.8558 mL	7.7116 mL	19.2790 mL
	10 mM	0.3856 mL	1.9279 mL	3.8558 mL	9.6395 mL
	15 mM	0.2571 mL	1.2853 mL	2.5705 mL	6.4263 mL
	20 mM	0.1928 mL	0.9639 mL	1.9279 mL	4.8197 mL
	25 mM	0.1542 mL	0.7712 mL	1.5423 mL	3.8558 mL
	30 mM	0.1285 mL	0.6426 mL	1.2853 mL	3.2132 mL
	40 mM	0.0964 mL	0.4820 mL	0.9639 mL	2.4099 mL
	50 mM	0.0771 mL	0.3856 mL	0.7712 mL	1.9279 mL
	60 mM	0.0643 mL	0.3213 mL	0.6426 mL	1.6066 mL
	80 mM	0.0482 mL	0.2410 mL	0.4820 mL	1.2049 mL
	100 mM	0.0386 mL	0.1928 mL	0.3856 mL	0.9639 mL

Pseudomonas quinolone signal Related Classifications

Inflammation/Immunology Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pseudomonas quinolone signal108985-27-9PQSBacterialpseudomonas aeruginosaregulatory bacterial toxicityInhibitorinhibitorinhibit

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Pseudomonas quinolone signal (Synonyms: PQS)

고객리뷰

Other Forms of Pseudomonas quinolone signal:

Pseudomonas quinolone signal Related Antibodies

2 Publications Citing Use of MCE Pseudomonas quinolone signal

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

순도&문서

References

고객리뷰

Pseudomonas quinolone signal Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Pseudomonas quinolone signal Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report