1. Recombinant Proteins
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. Angiopoietins
  4. Angiopoietin Like 3 Proteins
  5. ANGPTL3/Angiopoietin-like 3 Protein, Human (HEK293, His-Avi)

ANGPTL3/Angiopoietin-like 3 Protein, Human (HEK293, His-Avi)

Cat. No.: HY-P78379
Handling Instructions

ANGPTL3/Angiopoietin-like 3 protein, a hepatokine, intricately regulates lipid and glucose metabolism. It stimulates plasma triglycerides by inhibiting lipoprotein lipase (LPL), suppressing TG clearance through PCSK6 and FURIN recruitment, independent of proteolytic cleavage and unaffected by GPIHBP1. ANGPTL3 inhibits endothelial lipase, elevating HDL cholesterol, and activates adipocyte lipolysis. It selectively suppresses LPL in oxidative tissues, directing VLDL-TG to white adipose tissue, potentially cooperating with ANGPTL8 and ANGPTL4. ANGPTL3 also lowers LDL cholesterol independently of the canonical APOE and LDLR pathway, and may stimulate hypothalamic LPL activity. ANGPTL3/Angiopoietin-like 3 Protein, Human (HEK293, His-Avi) is the recombinant human-derived ANGPTL3/Angiopoietin-like 3 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of ANGPTL3/Angiopoietin-like 3 Protein, Human (HEK293, His-Avi) is 203 a.a., with molecular weight of 30-38 kDa.

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Description

ANGPTL3/Angiopoietin-like 3 protein, a hepatokine, intricately regulates lipid and glucose metabolism. It stimulates plasma triglycerides by inhibiting lipoprotein lipase (LPL), suppressing TG clearance through PCSK6 and FURIN recruitment, independent of proteolytic cleavage and unaffected by GPIHBP1. ANGPTL3 inhibits endothelial lipase, elevating HDL cholesterol, and activates adipocyte lipolysis. It selectively suppresses LPL in oxidative tissues, directing VLDL-TG to white adipose tissue, potentially cooperating with ANGPTL8 and ANGPTL4. ANGPTL3 also lowers LDL cholesterol independently of the canonical APOE and LDLR pathway, and may stimulate hypothalamic LPL activity. ANGPTL3/Angiopoietin-like 3 Protein, Human (HEK293, His-Avi) is the recombinant human-derived ANGPTL3/Angiopoietin-like 3 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of ANGPTL3/Angiopoietin-like 3 Protein, Human (HEK293, His-Avi) is 203 a.a., with molecular weight of 30-38 kDa.

Background

The ANGPTL3/Angiopoietin-like 3 protein functions as a hepatokine, intricately involved in the regulation of lipid and glucose metabolism. Proposed to play a role in directing energy substrates towards storage or oxidative tissues in response to food intake, ANGPTL3 stimulates plasma triglycerides (TG) by inhibiting lipoprotein lipase (LPL) activity, leading to suppressed TG clearance. This effect is achieved through the recruitment of proprotein convertases PCSK6 and FURIN to LPL, resulting in cleavage and dissociation of LPL from the cell surface. Howeverly, this regulatory function does not require ANGPTL3 proteolytic cleavage, is mediated by the N-terminal domain, and remains unaffected by GPIHBP1. Additionally, ANGPTL3 inhibits endothelial lipase, elevating plasma levels of high-density lipoprotein (HDL) cholesterol and phospholipids. By binding to adipocytes, it activates lipolysis, releasing free fatty acids and glycerol. ANGPTL3 selectively suppresses LPL in oxidative tissues, directing very low-density lipoprotein (VLDL)-TG to white adipose tissue (WAT) for storage in response to food, potentially cooperating with circulating, liver-derived ANGPTL8, and ANGPTL4 expression in WAT. It also contributes to lower plasma levels of low-density lipoprotein (LDL)-cholesterol independently of the canonical APOE and LDLR pathway. Moreover, ANGPTL3 may stimulate hypothalamic LPL activity, while in vitro, it inhibits LPL activity without effectiveness on GPIHBP1-stabilized LPL.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

Q9Y5C1 (S17-K219)

Gene ID
Molecular Construction
N-term
ANGPTL3 (S17-K219)
Accession # Q9Y5C1
His-Avi
C-term
Synonyms
ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3
Molecular Weight

30-38 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

ANGPTL3/Angiopoietin-like 3 Protein, Human (HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ANGPTL3/Angiopoietin-like 3 Protein, Human (HEK293, His-Avi)
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HY-P78379
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