1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors Neurotrophic Factors
  4. IL-1R IL-6R Neuropoietic cytokines
  5. gp130
  6. gp130/IL6ST Protein, Rat (HEK293, His)

gp130/IL6ST Protein, Rat (HEK293, His)

Cat. No.: HY-P76374
Handling Instructions
Top Publications Citing Use of Products
  • Technical Parameters

  • Properties

  • Documentation

Species

Rat

Source

HEK 293

Tag

His

Accession

H9BFG4 (M4-E618)

Gene ID

25205

Synonyms
Interleukin-6 receptor subunit beta; IL-6R-beta; IL-6RB; gp130; CD130
AA Sequence

Molecular Weight

90-100 kDa.

Purity

Greater than 97 % as determined by SDS-PAGE

Biological Activity
Measured by its ability to inhibit the IL-6Rα enhancement of IL-6 activity on M1 mouse myeloid leukemia cells and the ED50 is typically 0.5-2 μg/mL in the presence of 50 ng/mL human IL-6sR and 100 ng/mL human IL-6.
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
gp130/IL6ST Protein, Rat (HEK293, His)
Cat. No.:
HY-P76374
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