IGFBP-2 Protein, Human (HEK293, His)

IGFBP2 Protein, Human (HEK293, His) is a member of the family of high-affinity binding proteins (IGFBP1-6) and appears to play a governing role in Insulin-like Growth Factor (IGF) regulation in the central nervous system.

Insulin-like Growth Factor Binding Protein 2 (IGFBP2) is a member of the family of high-affinity binding proteins (IGFBP1-6) and appears to play a governing role in Insulin-like Growth Factor (IGF) regulation in the central nervous system^[1]. Insulin-like Growth Factor-Binding Proteins (IGFBPs) modulate the actions of secreted insulin-like growth facts (IGFs) by binding to them and increase the IGF halflife in the extracellular milieu and circulation by sequestering them in this bound form. IGFBPs either enhance or inhibit IGF actions on target cells; the individual IGFBPs either inhibit or potentiate IGF effects on osteoblasts in bone^[2].

1. The ED₅₀ is <2 µg/mL as measured by FDC-P1 cells.
2. Measured by its ability to inhibit the biological activity of IGF-II on MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.05-0.2 μg/ml in the presence of 14 ng/mL rhIGF-II.

• 
  Measured by its ability to inhibit the biological activity of IGF-II on MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.1501 μg/mL in the presence of 14 ng/mL rhIGF-II, corresponding to a specific activity is 6.662×10³ units/mg.

Human

HEK293

C-His

P18065 (F40-Q325)

3485  [NCBI]

Partial

FRCPPCTPERLAACGPPPVAPPAAVAAVAGGARMPCAELVREPGCGCCSVCARLEGEACGVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQ

Approximately 33-35 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.2 μm filtered solution of PBS.
2.Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Milanesi E, et al. Insulin-like growth factor binding protein 2 in bipolar disorder: An expression study in peripheral tissues. World J Biol Psychiatry. 2018 Dec;19(8):610-618.  [Content Brief]

  [2]. Richman C, et al. Recombinant human insulin-like growth factor-binding protein-5 stimulates bone formation parameters in vitro and in vivo. Endocrinology. 1999 Oct;140(10):4699-705.  [Content Brief]