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IGFBP-2 Protein, Rhesus Macaque (HEK293, His)

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Based on 1 Customer Validation

IGFBP‐2 is a member of a family of six insulin‐like growth factor (IGF) binding proteins. IGFBP-2 is the second most abundant IGFBP in the circulation and binds IGF-II with higher affinity than IGF-I. IGFBP-2 has been implicated in the regulation of IGF activity in most tissues and organs. IGFBP-2 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived IGFBP-2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rhesus Macaque
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IGFBP-2 Protein, Rhesus Macaque (HEK293, His)
  • Experimental Validation Results for IGFBP-2 Protein, Rhesus Macaque (HEK293, His)
  • IGFBP-2 Protein, Rhesus Macaque (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

IGFBP‐2 is a member of a family of six insulin‐like growth factor (IGF) binding proteins. IGFBP-2 is the second most abundant IGFBP in the circulation and binds IGF-II with higher affinity than IGF-I. IGFBP-2 has been implicated in the regulation of IGF activity in most tissues and organs. IGFBP-2 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived IGFBP-2 protein, expressed by HEK293 , with C-His labeled tag.

Background

IGFBP‐2 is a member of a family of six insulin‐like growth factor (IGF) binding proteins. IGFBP-2 is the second most abundant IGFBP in the circulation and binds IGF-II with higher affinity than IGF-I. IGFBP-2 has been implicated in the regulation of IGF activity in most tissues and organs. IGFBP‐2 is involved in regulation of insulin-like growth factor receptor signaling pathway[1][2][3][4].

Verified Bioactivity

Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED50 for this effect is 0.03282 µg/mL in the presence of 14 ng/mL rh IGF-I, corresponding to a specific activity is 3.047×104 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED50 for this effect is 0.03282 µg/mL in the presence of 14 ng/mL rh IGF-I, corresponding to a specific activity is 3.047×104 units/mg.

Technical Parameters

  • Species Rhesus Macaque
  • Source HEK293
  • Tag C-6*His
  • Accession

    XP_001087071.1 (E38-Q326)

  • Gene ID
    696214  [NCBI]
  • Molecular Construction
    • N-term
    • IGFBP-2 (E38-Q326)
      Accession # XP_001087071.1
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    IGFBP2; Insulin-Like Growth Factor Binding Protein 2 (36kD); Prev. IBP2; IGF-BP53; Insulin-Like Growth Factor-Binding Protein 2; IGFBP-2; Insulin-Like Growth Factor Binding Protein 2, 36kDa; IBP-2; IGF-Binding Protein 2; BP2; Insulin Like Growth Factor Bi

  • AA Sequence

    EVLFRCPPCTPERLAACGPPPVAPPAAVAAVAGGARMPCAELVREPGCGCCSVCARLEGEACGVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVAEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEAHGVHTQRMQ

  • Predicted Molecular Mass

    31.3 kDa

  • Molecular Weight

    Approximately 33 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED50 for this effect is 0.03282 µg/mL in the presence of 14 ng/mL rh IGF-I, corresponding to a specific activity is 3.047×104 units/mg.

  • IGFBP-2 Protein, Rhesus Macaque (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for IGFBP-2 Protein, Rhesus Macaque (HEK293, His)
  • Experimental Validation Results for IGFBP-2 Protein, Rhesus Macaque (HEK293, His)
  • IGFBP-2 Protein, Rhesus Macaque (HEK293, His) Product Image