IGFBP-2 Protein, Mouse (HEK293, Fc)
Based on 1 Customer Validation
IGFBP-2 protein, a member of the IGF-binding protein family, inhibits IGF-mediated growth and developmental rates. It can extend the half-life of IGF and exhibit dual functions by inhibiting or stimulating the growth-promoting effect of IGF in cell culture. IGFBP-2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived IGFBP-2 protein, expressed by HEK293 , with C-hFc labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
IGFBP-2 protein, a member of the IGF-binding protein family, inhibits IGF-mediated growth and developmental rates. It can extend the half-life of IGF and exhibit dual functions by inhibiting or stimulating the growth-promoting effect of IGF in cell culture. IGFBP-2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived IGFBP-2 protein, expressed by HEK293 , with C-hFc labeled tag.
Background
IGFBP-2 protein acts as an inhibitor, specifically targeting IGF-mediated growth and developmental rates. As a member of the IGF-binding protein family, IGFBP-2 prolongs the half-life of IGFs and exhibits dual functionality, either inhibiting or stimulating the growth-promoting effects of IGFs in cell culture. This modulation occurs through the alteration of the interaction between IGFs and their cell surface receptors. Notably, IGFBP-2 demonstrates a higher binding affinity for IGF2 compared to IGF1, underscoring its role in finely regulating the intricate balance of IGF signaling pathways.
Verified Bioactivity
Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 0.05- 0.4 μg/mL in the presence of 14 ng/mL human IGFII.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-hFc
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Accession
P47877 (E35-Q305)
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Molecular Construction
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N-term
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IGFBP-2 (E35-Q305)
Accession # P47877 -
hFc
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
IGFBP2; Insulin-Like Growth Factor Binding Protein 2 (36kD); Prev. IBP2; IGF-BP53; Insulin-Like Growth Factor-Binding Protein 2; IGFBP-2; Insulin-Like Growth Factor Binding Protein 2, 36kDa; IBP-2; IGF-Binding Protein 2; BP2; Insulin Like Growth Factor Bi
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AA Sequence
EVLFRCPPCTPERLAACGPPPDAPCAELVREPGCGCCSVCARQEGEACGVYIPRCAQTLRCYPNPGSELPLKALVTGAGTCEKRRVGTTPQQVADSDDDHSEGGLVENHVDGTMNMLGGGSSAGRKPLKSGMKELAVFREKVNEQHRQMGKGAKHLSLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDDRGPLEHLYSLHIPNCDKHGRYNLKQCKMSLNGQRGECWCVNPNTGKPIQGAPTIRGDPECHLFYNEQQETGGAHAQSVQ
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Predicted Molecular Mass
56.6 kDa
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Molecular Weight
Approximately 50-66 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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