IGFBP-2 Protein, Mouse (HEK293, Fc)

IGFBP-2 protein, a member of the IGF-binding protein family, inhibits IGF-mediated growth and developmental rates. It can extend the half-life of IGF and exhibit dual functions by inhibiting or stimulating the growth-promoting effect of IGF in cell culture. IGFBP-2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived IGFBP-2 protein, expressed by HEK293 , with C-hFc labeled tag.

IGFBP-2 protein acts as an inhibitor, specifically targeting IGF-mediated growth and developmental rates. As a member of the IGF-binding protein family, IGFBP-2 prolongs the half-life of IGFs and exhibits dual functionality, either inhibiting or stimulating the growth-promoting effects of IGFs in cell culture. This modulation occurs through the alteration of the interaction between IGFs and their cell surface receptors. Notably, IGFBP-2 demonstrates a higher binding affinity for IGF2 compared to IGF1, underscoring its role in finely regulating the intricate balance of IGF signaling pathways.

Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells. The ED₅₀ for this effect is typically 0.05- 0.4 μg/mL in the presence of 14 ng/mL human IGFII.

Mouse

HEK293

C-hFc

P47877 (E35-Q305)

16008  [NCBI]

Full Length of Mature Protein

EVLFRCPPCTPERLAACGPPPDAPCAELVREPGCGCCSVCARQEGEACGVYIPRCAQTLRCYPNPGSELPLKALVTGAGTCEKRRVGTTPQQVADSDDDHSEGGLVENHVDGTMNMLGGGSSAGRKPLKSGMKELAVFREKVNEQHRQMGKGAKHLSLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDDRGPLEHLYSLHIPNCDKHGRYNLKQCKMSLNGQRGECWCVNPNTGKPIQGAPTIRGDPECHLFYNEQQETGGAHAQSVQ

Approximately 50-66 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.