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IGFBP-2 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

IGFBP-2 protein, a member of the IGF-binding protein family, inhibits IGF-mediated growth and developmental rates. It can extend the half-life of IGF and exhibit dual functions by inhibiting or stimulating the growth-promoting effect of IGF in cell culture. IGFBP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IGFBP-2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IGFBP-2 Protein, Mouse (HEK293, His)
  • IGFBP-2 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

IGFBP-2 protein, a member of the IGF-binding protein family, inhibits IGF-mediated growth and developmental rates. It can extend the half-life of IGF and exhibit dual functions by inhibiting or stimulating the growth-promoting effect of IGF in cell culture. IGFBP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IGFBP-2 protein, expressed by HEK293 , with C-His labeled tag.

Background

IGFBP-2 protein acts as an inhibitor, specifically targeting IGF-mediated growth and developmental rates. As a member of the IGF-binding protein family, IGFBP-2 prolongs the half-life of IGFs and exhibits dual functionality, either inhibiting or stimulating the growth-promoting effects of IGFs in cell culture. This modulation occurs through the alteration of the interaction between IGFs and their cell surface receptors. Notably, IGFBP-2 demonstrates a higher binding affinity for IGF2 compared to IGF1, underscoring its role in finely regulating the intricate balance of IGF signaling pathways.

Verified Bioactivity

Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED50 for this effect is 0.2342 µg/mL in the presence of 30 ng/mL IGF-I, corresponding to a specific activity is 4.269×103 units/mg.

MCE Validation Data

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED50 for this effect is 0.2342 µg/mL in the presence of 30 ng/mL IGF-I, corresponding to a specific activity is 4.269×103 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    P47877 (E35-Q305)

  • Gene ID
    16008  [NCBI]
  • Molecular Construction
    • N-term
    • IGFBP-2 (E35-Q305)
      Accession # P47877
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    IGFBP2; Insulin-Like Growth Factor Binding Protein 2 (36kD); Prev. IBP2; IGF-BP53; Insulin-Like Growth Factor-Binding Protein 2; IGFBP-2; Insulin-Like Growth Factor Binding Protein 2, 36kDa; IBP-2; IGF-Binding Protein 2; BP2; Insulin Like Growth Factor Bi

  • AA Sequence

    EVLFRCPPCTPERLAACGPPPDAPCAELVREPGCGCCSVCARQEGEACGVYIPRCAQTLRCYPNPGSELPLKALVTGAGTCEKRRVGTTPQQVADSDDDHSEGGLVENHVDGTMNMLGGGSSAGRKPLKSGMKELAVFREKVNEQHRQMGKGAKHLSLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDDRGPLEHLYSLHIPNCDKHGRYNLKQCKMSLNGQRGECWCVNPNTGKPIQGAPTIRGDPECHLFYNEQQETGGAHAQSVQ

  • Predicted Molecular Mass

    31 kDa

  • Molecular Weight

    Approximately 32-40 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED50 for this effect is 0.2342 µg/mL in the presence of 30 ng/mL IGF-I, corresponding to a specific activity is 4.269×103 units/mg.

  • IGFBP-2 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for IGFBP-2 Protein, Mouse (HEK293, His)
  • IGFBP-2 Protein, Mouse (HEK293, His) Product Image