1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. Dendritic Cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. SIRP alpha/CD172a Immunoglobulin-like Cell Adhesion Molecules
  5. Signal Regulatory Protein α
  6. SIRP alpha/CD172a Protein, Rat (HEK293, Fc)

SIRP alpha/CD172a Protein, Rat (HEK293, Fc)

Cat. No.: HY-P76072
Handling Instructions

When paired with KLRD1, the NKG2C/CD159c protein recognizes MHC-E loaded with MHC-G peptides, thereby contributing to NK cell generation and function. It also regulates cytotoxic lymphocytes in viral infections. SIRP alpha/CD172a Protein, Rat (HEK293, Fc) is the recombinant rat-derived SIRP alpha/CD172a protein, expressed by HEK293 , with C-hFc labeled tag. The total length of SIRP alpha/CD172a Protein, Rat (HEK293, Fc) is 373 a.a., with molecular weight of ~64.25 kDa.

For research use only. We do not sell to patients.

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Description

When paired with KLRD1, the NKG2C/CD159c protein recognizes MHC-E loaded with MHC-G peptides, thereby contributing to NK cell generation and function. It also regulates cytotoxic lymphocytes in viral infections. SIRP alpha/CD172a Protein, Rat (HEK293, Fc) is the recombinant rat-derived SIRP alpha/CD172a protein, expressed by HEK293 , with C-hFc labeled tag. The total length of SIRP alpha/CD172a Protein, Rat (HEK293, Fc) is 373 a.a., with molecular weight of ~64.25 kDa.

Background

SIRP alpha/CD172a protein, an immunoglobulin-like cell surface receptor for CD47, serves as a versatile mediator in various cellular processes. Functioning as a docking protein, it induces the translocation of PTPN6, PTPN11, and other binding partners from the cytosol to the plasma membrane. Beyond its role in cellular adhesion, SIRP alpha supports the adhesion of cerebellar neurons, neurite outgrowth, and glial cell attachment. Additionally, it plays a potential key role in intracellular signaling during synaptogenesis and synaptic function. Involved in negative regulation, SIRP alpha regulates receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors, or insulin. It further mediates negative regulation of phagocytosis, mast cell activation, and dendritic cell activation. Notably, CD47 binding prevents the maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. SIRP alpha's significance extends to antiviral immunity, as it limits new world arenavirus infection by decreasing virus internalization. Acting as a receptor for THBS1, it stimulates the phosphorylation of SIRPA and, in response to THBS1, is involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production. SIRP alpha engages in diverse interactions with proteins such as PTPN11, GRB2, FGR, JAK2, SCAP1, SCAP2, FYB1, PTK2B, and TRIM2, illustrating its intricate role as a central player in cellular signaling and immune responses.

Species

Rat

Source

HEK293

Tag

C-hFc

Accession

P97710 (M1-N373)

Gene ID

25528  [NCBI]

Molecular Construction
N-term
CD172a (M1-N373)
Accession # P97710
hFc
C-term
Synonyms
Signal-regulatory protein alpha; CD172a; SIRP alpha; SIRPA; MFR; SHPS1; SIRP
Molecular Weight

Approximately 64.25 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SIRP alpha/CD172a Protein, Rat (HEK293, Fc)
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HY-P76072
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