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  4. S-Nitroso-N-acetyl-DL-penicillamine

S-Nitroso-N-acetyl-DL-penicillamine  (Synonyms: SNAP)

Cat. No.: HY-121526 Purity: 98.76%
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S-Nitroso-N-acetyl-DL-penicillamine (SNAP) is a nitric oxide donor and acts as a stable inhibitor of platelet aggregation.

For research use only. We do not sell to patients.

CAS No. : 67776-06-1

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Customer Review

Based on 7 publication(s) in Google Scholar

S-Nitroso-N-acetyl-DL-penicillamine Related Antibodies

Top Publications Citing Use of Products

    S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: Aging Cell. 2025 Feb 6:e14502.  [Abstract]

    F-AML12 culture with NO donor S-Nitroso-N-acetyl-DL-penicillamine (SNAP, 100 μM, 24 h) assay for triglyceride concentration.

    S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: Aging Cell. 2025 Feb 6:e14502.  [Abstract]

    F-AML12 culture with NO donor S-Nitroso-N-acetyl-DL-penicillamine (SNAP, 100 μM, 24 h) assay for triglyceride concentration.

    S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: Aging Cell. 2025 Feb 6:e14502.  [Abstract]

    Aging resulted in a remarkable increment in SVR of MASH rats as compared to the control rats in the presence of ACH or S-Nitroso-N-acetyl-DL-penicillamine (SNAP).

    S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Oct 1;134(22):e181368.  [Abstract]

    NRP2-depleted BT549 cells were treated with either DMSO or the NO donor S-Nitroso-N-acetyl-DL-penicillamine (SNAP, 50 μM) for 24 hours and NFE2L2 localization was assessed by immunofluorescence.

    S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: Hepatol Commun. 2023 Dec 22;8(1):e0350.  [Abstract]

    Primary HSCs isolated from young mice were cultured alone, with the addition of L-NAME, cocultured with the indicated primary LSECs, or cocultured with the indicated LSECs with the addition of L-NAME and SNAP for 3 days. Representative images of cultured primary HSCs.

    S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: Hepatol Commun. 2023 Dec 22;8(1):e0350.  [Abstract]

    Primary HSCs isolated from young mice were cultured alone, with the addition of L-NAME, cocultured with the indicated primary LSECs, or cocultured with the indicated LSECs with the addition of L-NAME and SNAP for 3 days. Western blotting analysis of α-SMA expression levels.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    S-Nitroso-N-acetyl-DL-penicillamine (SNAP) is a nitric oxide donor and acts as a stable inhibitor of platelet aggregation[1][2][3][4].

    In Vitro

    S-Nitroso-N-acetyl-DL-penicillamine (10 mM; 8 hours) induces toxicity of about 80% after 6 hours under normoxic conditions by releasing nitric oxide (NO)[1].
    S-Nitroso-N-acetyl-DL-penicillamine has a half-time about 6 hours in in isolated rat ventricular myocytes[3].
    S-Nitroso-N-acetyl-DL-penicillamine (100 μM; 30 minutes) causes sustained decrease in the basal pHi in isolated rat ventricular myocytes[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    S-Nitroso-N-acetyl-DL-penicillamine Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Rat liver sinusoidal endothelial cells
    Concentration: 2 mM, 5 mM, 10 mM
    Incubation Time: 2 hours, 4 hours, 6 hours, 8 hours
    Result: Exhibited cytotoxicity against cultivated endothelial cells.
    In Vivo

    SNAP (100μM, 300μM) causes small but significant increases of the electrically evoked [3H]-acetylcholine release in guinea-pig tracheal[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    220.25

    Formula

    C7H12N2O4S
    CAS No.
    Appearance

    Solid

    Color

    Light green to green

    SMILES

    O=NSC(C)(C(C(O)=O)NC(C)=O)C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : 175 mg/mL (794.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 11.11 mg/mL (50.44 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.5403 mL 22.7015 mL 45.4030 mL
    5 mM 0.9081 mL 4.5403 mL 9.0806 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (9.44 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (9.44 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *The compound is unstable in solutions, freshly prepared is recommended.

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.76%

    SDS (580 KB)

    COA (249 KB) HNMR (235 KB) RP-HPLC (248 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 4.5403 mL 22.7015 mL 45.4030 mL 113.5074 mL
    5 mM 0.9081 mL 4.5403 mL 9.0806 mL 22.7015 mL
    10 mM 0.4540 mL 2.2701 mL 4.5403 mL 11.3507 mL
    15 mM 0.3027 mL 1.5134 mL 3.0269 mL 7.5672 mL
    20 mM 0.2270 mL 1.1351 mL 2.2701 mL 5.6754 mL
    25 mM 0.1816 mL 0.9081 mL 1.8161 mL 4.5403 mL
    30 mM 0.1513 mL 0.7567 mL 1.5134 mL 3.7836 mL
    40 mM 0.1135 mL 0.5675 mL 1.1351 mL 2.8377 mL
    50 mM 0.0908 mL 0.4540 mL 0.9081 mL 2.2701 mL
    DMSO 60 mM 0.0757 mL 0.3784 mL 0.7567 mL 1.8918 mL
    80 mM 0.0568 mL 0.2838 mL 0.5675 mL 1.4188 mL
    100 mM 0.0454 mL 0.2270 mL 0.4540 mL 1.1351 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    S-Nitroso-N-acetyl-DL-penicillamine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    S-Nitroso-N-acetyl-DL-penicillamine67776-06-1SNAPNO SynthaseNitric oxide synthasesNOSNOInhibitorinhibitorinhibit

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