S-Nitroso-N-acetyl-DL-penicillamine
Based on 7 publication(s) in Google Scholar
S-Nitroso-N-acetyl-DL-penicillamine (SNAP) is a nitric oxide donor and acts as a stable inhibitor of platelet aggregation.
For research use only. We do not sell to patients.
- Purity: 98.76%
- CAS No.: 67776-06-1
- Formula: C7H12N2O4S
- Molecular Weight:220.25
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Storage:Powder -20°C, 3 years , 4°C, 2 years
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) S-Nitroso-N-acetyl-DL-penicillamine
More- J Clin Invest. 2024 Oct 1;134(22):e181368. [Abstract]
- EMBO Mol Med. 2025 Jan;17(1):85-111. [Abstract]
- Aging Cell. 2025 May;24(5):e14502. [Abstract]
- Hepatol Commun. 2023 Dec 22;8(1):e0350. [Abstract]
- Karolinska Institutet. 2025 Feb.
- Pediatr Discov. 2024 Jun 28;2(4):e95. [Abstract]
- University of Jena. 2023 Dec.
Customer Validation & Images
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Cell Imaging/Staining
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Bio/Physico-chemical Assay
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In Vivo Efficacy Study
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IF
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Cell Imaging/Staining
Biological Activity
S-Nitroso-N-acetyl-DL-penicillamine (10 mM; 8 hours) induces toxicity of about 80% after 6 hours under normoxic conditions by releasing nitric oxide (NO)[1].
S-Nitroso-N-acetyl-DL-penicillamine has a half-time about 6 hours in in isolated rat ventricular myocytes[3].
S-Nitroso-N-acetyl-DL-penicillamine (100 μM; 30 minutes) causes sustained decrease in the basal pHi in isolated rat ventricular myocytes[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Rat liver sinusoidal endothelial cells
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Concentration:2 mM, 5 mM, 10 mM
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Incubation Time:2 hours, 4 hours, 6 hours, 8 hours
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Result:Exhibited cytotoxicity against cultivated endothelial cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 67776-06-1
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Appearance Solid
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Molecular Weight 220.25
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Formula C7H12N2O4S
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Color Light green to green
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SMILES
O=NSC(C)(C(C(O)=O)NC(C)=O)C
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Synonyms
SNAP
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years * The compound is unstable in solutions, freshly prepared is recommended.
Publications (7)
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Journal Impact Factor
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Most Recent
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J Clin Invest
Neuropilin-2-expressing breast cancer cells mitigate radiation-induced oxidative stress through nitric oxide signaling. [Abstract]2024 Oct 1;134(22):e181368. PMID: 39352757
S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Oct 1;134(22):e181368. [Abstract]
NRP2-depleted BT549 cells were treated with either DMSO or the NO donor S-Nitroso-N-acetyl-DL-penicillamine (SNAP, 50 μM) for 24 hours and NFE2L2 localization was assessed by immunofluorescence.
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EMBO Mol Med
A novel anti-epileptogenesis strategy of temporal lobe epilepsy based on nitric oxide donor. [Abstract]2025 Jan;17(1):85-111. PMID: 39653809 -
Aging Cell
Aging-Associated Liver Sinusoidal Endothelial Cells Dysfunction Aggravates the Progression of Metabolic Dysfunction-Associated Steatotic Liver Disease. [Abstract]2025 May;24(5):e14502. PMID: 39912563
S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: Aging Cell. 2025 May;24(5):e14502. [Abstract]
F-AML12 culture with NO donor S-Nitroso-N-acetyl-DL-penicillamine (SNAP, 100 μM, 24 h) assay for triglyceride concentration.
S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: Aging Cell. 2025 May;24(5):e14502. [Abstract]
F-AML12 culture with NO donor S-Nitroso-N-acetyl-DL-penicillamine (SNAP, 100 μM, 24 h) assay for triglyceride concentration.
S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: Aging Cell. 2025 May;24(5):e14502. [Abstract]
Aging resulted in a remarkable increment in SVR of MASH rats as compared to the control rats in the presence of ACH or S-Nitroso-N-acetyl-DL-penicillamine (SNAP).
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Hepatol Commun
Aging aggravates liver fibrosis through downregulated hepatocyte SIRT1-induced liver sinusoidal endothelial cell dysfunction. [Abstract]2023 Dec 22;8(1):e0350. PMID: 38126919
S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: Hepatol Commun. 2023 Dec 22;8(1):e0350. [Abstract]
Primary HSCs isolated from young mice were cultured alone, with the addition of L-NAME, cocultured with the indicated primary LSECs, or cocultured with the indicated LSECs with the addition of L-NAME and SNAP for 3 days. Representative images of cultured primary HSCs.
S-Nitroso-N-acetyl-DL-penicillamine purchased from MedChemExpress. Usage Cited in: Hepatol Commun. 2023 Dec 22;8(1):e0350. [Abstract]
Primary HSCs isolated from young mice were cultured alone, with the addition of L-NAME, cocultured with the indicated primary LSECs, or cocultured with the indicated LSECs with the addition of L-NAME and SNAP for 3 days. Western blotting analysis of α-SMA expression levels.
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Pediatr Discov
Nitric oxide donor S-Nitroso-N-acetyl penicillamine for hepatic stellate cells to restore quiescence. [Abstract]2024 Jun 28;2(4):e95. PMID: 40626123 -
Solvent & Solubility
DMSO : 175 mg/mL (794.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 11.11 mg/mL (50.44 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (9.44 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (9.44 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * The compound is unstable in solutions, freshly prepared is recommended.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. E. Salas, et al. Comparative pharmacology of analogues of S-nitroso-N-acetyl-DL-penicillamine on human platelets. Br J Pharmacol. 1994 Aug;112(4):1071-6. [Content Brief]
[2]. Ioannidis I, et al. Enhanced release of nitric oxide causes increased cytotoxicity of S-nitroso-N-acetyl-DL-penicillamine and sodium nitroprusside under hypoxic conditions. Biochem J. 1996 Sep 15;318 ( Pt 3):789-95. [Content Brief]
[3]. Pravdic D, et al. Effect of nitric oxide donors S-nitroso-N-acetyl-DL-penicillamine, spermine NONOate and propylamine propylamine NONOate on intracellular pH in cardiomyocytes. Clin Exp Pharmacol Physiol. 2012 Sep;39(9):772-8. [Content Brief]
[4]. Mang CF, et al. Modulation of acetylcholine release in the guinea-pig trachea by the nitric oxide donor, S-nitroso-N-acetyl-DL-penicillamine (SNAP). Br J Pharmacol. 2000 Sep;131(1):94-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 4.5403 mL | 22.7015 mL | 45.4030 mL | 113.5074 mL |
| 5 mM | 0.9081 mL | 4.5403 mL | 9.0806 mL | 22.7015 mL | |
| 10 mM | 0.4540 mL | 2.2701 mL | 4.5403 mL | 11.3507 mL | |
| 15 mM | 0.3027 mL | 1.5134 mL | 3.0269 mL | 7.5672 mL | |
| 20 mM | 0.2270 mL | 1.1351 mL | 2.2701 mL | 5.6754 mL | |
| 25 mM | 0.1816 mL | 0.9081 mL | 1.8161 mL | 4.5403 mL | |
| 30 mM | 0.1513 mL | 0.7567 mL | 1.5134 mL | 3.7836 mL | |
| 40 mM | 0.1135 mL | 0.5675 mL | 1.1351 mL | 2.8377 mL | |
| 50 mM | 0.0908 mL | 0.4540 mL | 0.9081 mL | 2.2701 mL | |
| DMSO | 60 mM | 0.0757 mL | 0.3784 mL | 0.7567 mL | 1.8918 mL |
| 80 mM | 0.0568 mL | 0.2838 mL | 0.5675 mL | 1.4188 mL | |
| 100 mM | 0.0454 mL | 0.2270 mL | 0.4540 mL | 1.1351 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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