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  5. Enzyme Substrates
  6. Suc-AAPF-pNA

Suc-AAPF-pNA  (Synonyms: Suc-Ala-Ala-Pro-Phe-pNA)

製品番号: HY-P2573 純度: 99.55%
COA 取扱説明書 Technical Support

Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a chromogenic p-nitroanilide (pNA) substrate with the Km of 1.7 mM. Cleavage of Suc-AAPF-pNA releases 4-nitroaniline, which is yellow in colour and can be measured spectrophotometrically. Suc-AAPF-pNA can be used for the measurement of free and membrane-bound cathepsin G in human neutrophils.

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カスタムペプチド

CAS 番号 : 70967-97-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
5 mg $32 在庫あり
10 mg $50 在庫あり
25 mg $90 在庫あり
100 mg $260 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 2 publication(s) in Google Scholar

Suc-AAPF-pNA 関連抗体

Top Publications Citing Use of Products

    Suc-AAPF-pNA purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Oct 17;331(Pt 1):148374.  [Abstract]

    Predicted binding free energies and interaction modes between the three proteases and various substrates (Suc-AAPA-pNA, Suc-AAPI-pNA, Suc-AAPL-pNA and Suc-AAPF-pNA).
    aUnits: kcal/mol.

    Suc-AAPF-pNA purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2025 Oct 23:117451.  [Abstract]

    HEK293 cells were pretreated with Suc-AAPF-pNA (5 uM) and then stimulated with MRGPRX2 agonist C48/80 (30 ug/mL). The fluorescence intensity was recorded for 2 min. Suc-AAPF-pNA (5 uM) exhibited inhibitory effects on MRGPRX2-mediated calcium mobilization.

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    製品説明

    Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a chromogenic p-nitroanilide (pNA) substrate with the Km of 1.7 mM. Cleavage of Suc-AAPF-pNA releases 4-nitroaniline, which is yellow in colour and can be measured spectrophotometrically. Suc-AAPF-pNA can be used for the measurement of free and membrane-bound cathepsin G in human neutrophils[1][2][3].

    体外実験

    The elastase activity is measured as activity against the substrate Suc-AAPF-Pna. Aliquots (10 μL) of SmCTF samples are incubated at 30 °C with 200 μM Suc-AAPF-pNA and the activity of cercarial elastase measured as abosorbance at 400 nm[2].
    Proteolytic activity is measured by hydrolysis of the substrate suc-AAPF-pNA. Serial dilutions of the proteases are made in 100 mM Tris buffer, pH 8.6, containing 10 mM CaCl2. Enzyme activity is measured at 25°C in 100 mM Tris/HCl, pH 8.6 containing 1.6 mM suc-AAPF-pNA, 0.0005% Tween-80 and 1% DMSO by monitoring the absorbance change at 410 nm using a spectrophotometer. Each dilution of enzyme is tested in triplicate[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Suc-AAPF-pNA 関連抗体
    分子量

    624.64

    分子式

    C30H36N6O9
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    {Suc}-Ala-Ala-Pro-Phe-{pNA}
    Sequence Shortening

    Suc-AAPF-pNA
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    polypeptide, sealed storage, away from moisture and light, under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (polypeptide, sealed storage, away from moisture and light, under nitrogen)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (160.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6009 mL 8.0046 mL 16.0092 mL
    5 mM 0.3202 mL 1.6009 mL 3.2018 mL
    10 mM 0.1601 mL 0.8005 mL 1.6009 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.00 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.00 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.55%

    COA (249 KB) Elemental Analysis Report (108 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6009 mL 8.0046 mL 16.0092 mL 40.0231 mL
    5 mM 0.3202 mL 1.6009 mL 3.2018 mL 8.0046 mL
    10 mM 0.1601 mL 0.8005 mL 1.6009 mL 4.0023 mL
    15 mM 0.1067 mL 0.5336 mL 1.0673 mL 2.6682 mL
    20 mM 0.0800 mL 0.4002 mL 0.8005 mL 2.0012 mL
    25 mM 0.0640 mL 0.3202 mL 0.6404 mL 1.6009 mL
    30 mM 0.0534 mL 0.2668 mL 0.5336 mL 1.3341 mL
    40 mM 0.0400 mL 0.2001 mL 0.4002 mL 1.0006 mL
    50 mM 0.0320 mL 0.1601 mL 0.3202 mL 0.8005 mL
    60 mM 0.0267 mL 0.1334 mL 0.2668 mL 0.6671 mL
    80 mM 0.0200 mL 0.1001 mL 0.2001 mL 0.5003 mL
    100 mM 0.0160 mL 0.0800 mL 0.1601 mL 0.4002 mL
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    Suc-AAPF-pNA Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Suc-AAPF-pNA70967-97-4Suc-Ala-Ala-Pro-Phe-pNAOtherschromogenic pNA substratefree and membrane-bound cathepsin GInhibitorinhibitorinhibit

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    製品名:
    Suc-AAPF-pNA
    製品番号:
    HY-P2573
    数量:
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