Sulfosuccinimidyl oleate sodium
Based on 45 publication(s) in Google Scholar
Sulfosuccinimidyl oleate sodium (Sulfo-N-succinimidyl oleate sodium) is a long chain fatty acid that inhibits fatty acid transport into cells. Sulfosuccinimidyl oleate sodium is a potent and irreversible inhibitor of mitochondrial respiratory chain. Sulfosuccinimidyl oleate sodium binds the CD36 receptor on the surface of microglia. Anti-inflammatory effect.
For research use only. We do not sell to patients.
- Purity: 99.66%
- Formula: C22H36NNaO7S
- Molecular Weight:481.58
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications Citing Use of MedChemExpress (MCE) Sulfosuccinimidyl oleate sodium
More- Adv Mater. 2026 Jun;38(33):e21108. [Abstract]
- Cancer Res. 2025 Apr 28:OF1-OF13. [Abstract]
- Nat Commun. 2026 May 11;17(1):4190. [Abstract]
- ACS Nano. 2026 Jun 2;20(21):15384-15409. [Abstract]
- Redox Biol. 2025 Mar:80:103528. [Abstract]
- Adv Sci (Weinh). 2026 Jun 2:e75875. [Abstract]
- Cell Rep Med. 2024 Jun 18;5(6):101592. [Abstract]
- Cell Death Dis. 2025 Apr 14;16(1):291. [Abstract]
- Cell Death Dis. 2024 Aug 26;15(8):623. [Abstract]
- Cancer Lett. 2024 Jan 28:581:216511. [Abstract]
- J Exp Med. 2023 Mar 6;220(3):e20221316. [Abstract]
- J Transl Med. 2023 Feb 6;21(1):89. [Abstract]
- Food Res Int. 2026 Mar 31:228:118352. [Abstract]
- Food Res Int. 2023 Nov;173(Pt 1):113328. [Abstract]
- Int J Biol Macromol. 2023 Dec 31;253(Pt 8):127324. [Abstract]
- Cell Rep. 2026 Feb 24;45(2):116952. [Abstract]
- Cell Rep. 2024 Oct 19;43(11):114881. [Abstract]
- Cell Rep. 2024 Jul 9;43(7):114465. [Abstract]
- J Invest Dermatol. 2025 Nov 20:S0022-202X(25)03555-9. [Abstract]
- Cancer Cell Int. 2025 May 14;25(1):176. [Abstract]
- JCI Insight. 2025 Feb 18;10(6):e184935. [Abstract]
- J Agric Food Chem. 2026 Feb 11;74(5):4407-4419. [Abstract]
- J Agric Food Chem. 2024 Jul 24;72(29):16287-16297. [Abstract]
- CNS Neurosci Ther. 2023 Jun;29 Suppl 1(Suppl 1):161-184. [Abstract]
- Cancer Metab. 2025 Feb 7;13(1):6. [Abstract]
- Nutrients. 2025 Feb 25;17(5):794. [Abstract]
- Int Immunopharmacol. 2026 Apr 27:181:116718. [Abstract]
- Int Immunopharmacol. 2026 Mar 1:172:116231. [Abstract]
- Int Immunopharmacol. 2025 Dec 10:167:115594. [Abstract]
- Int J Mol Sci. 2025 Apr 8;26(8):3482. [Abstract]
- Int J Mol Sci. 2023 Aug 31;24(17):13516. [Abstract]
- Front Pharmacol. 2020 Dec 16:11:593832. [Abstract]
- Toxicol Sci. 2023 Feb 17;191(2):276-284. [Abstract]
- Food Sci Nutr. 2023 Nov 22;12(2):1318-1329. [Abstract]
- BMC Cancer. 2023 Apr 21;23(1):367. [Abstract]
- Toxins. 2021 Nov 13;13(11):802. [Abstract]
- Cell Immunol. 2022 Feb:372:104475. [Abstract]
- Fish Physiol Biochem. 2025 Nov 24;51(6):197. [Abstract]
- bioRxiv. 2026 May 29.
- SSRN. 2025 Oct 22.
- University of Chicago. 2025 Aug.
- Heliyon. 2024 Nov 28;10(23):e40806. [Abstract]
- Research Square Preprint. 2023 Oct 11.
- SSRN. 2023 Aug 30.
- Research Square Print. December 29th, 2022.
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IF
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Cell Imaging/Staining
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In Vivo Imaging
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WB
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In Vivo Efficacy Study
Biological Activity
Sulfosuccinimidyl oleate (20 μM and 50 μM, 24 hours) alone does not alter the cellular viability. Exposure to 100 ng/ml LPS+5 ng/mL IFNγ modestly, yet significantly reduces the viability of the BV2 cells. Co-treatment with Sulfosuccinimidyl oleate prevents the LPS+IFNγ-induced reduction in the cell viability[1].
Sulfosuccinimidyl oleate (50 μM, 24 hours) co-treatment significantly reduces the LPS+IFNγ-induced expression of NOS2 and COX-2 in BV2 cells. Western blot analysis reveals a significant LPS/IFNγ-induced upregulation in the phosphorylated form of the p38, which is prevented by co-treatment with Sulfosuccinimidyl oleate (50 μM, 24 hours)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BV2 cells
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Concentration:20 μM and 50 μM
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Incubation Time:24 hours
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Result:Did not alter the viability of BV2 cells alone. Exposure of BV2 cells to 100 ng/mL LPS and 5 ng/mL IFNγ significantly reduced the viability of BV2 cells while simultaneous treatment with Sulfosuccinimidyl oleate prevented it.
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Cell Line:BV2 cells
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Concentration:50 μM
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Incubation Time:24 hours
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Result:Drastically increased the levels of NOS2, COX-2, and P-p38/T-p38.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:4-month-old male BALB/cABom mice with pMCAo model[1]
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Dosage:50 mg/kg
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Administration:Administered once by single oral gavage
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Result:Reduced brain damage following ischemia. Attenuated infarct size.
Chemical Information
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Appearance Solid
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Molecular Weight 481.58
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Formula C22H36NNaO7S
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Color White to off-white
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SMILES
O=S(C(C1)C(N(OC(CCCCCCC/C=C\CCCCCCCC)=O)C1=O)=O)(O[Na])=O
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Synonyms
Sulfo-N-succinimidyl oleate sodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (45)
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Journal Impact Factor
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Most Recent
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Adv Mater
Conformationally Variable Peptides Trap and Detoxify Ox-LDL in Plaques for Attenuating Atherosclerosis in Multiple Species. [Abstract]2026 Jun;38(33):e21108. PMID: 42116761 -
Cancer Res
Blocking the TCA Cycle in Cancer Cells Potentiates CD36+ T-cell-Mediated Antitumor Immunity by Suppressing ER Stress-Associated THBS2 Signaling. [Abstract]2025 Apr 28:OF1-OF13. PMID: 40293258 -
Nat Commun
Ascites protects against ferroptosis and enables the peritoneal growth of ovarian cancer. [Abstract]2026 May 11;17(1):4190. PMID: 42115216 -
ACS Nano
Injectable Binary-Amplified Cascade Hydrogels Suppress Post-Incomplete Microwave Ablation Relapse via Integrated Metallo-Metabolic-Immunomodulation. [Abstract]2026 Jun 2;20(21):15384-15409. PMID: 42150126 -
Redox Biol
Niraparib restricts intraperitoneal metastases of ovarian cancer by eliciting CD36-dependent ferroptosis. [Abstract]2025 Mar:80:103528. PMID: 39922130
Sulfosuccinimidyl oleate sodium purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar:80:103528. [Abstract]
OVCAR8 cells were cultured in fatty acid-free media supplemented with 1.0 μL/mL exogenous fatty acids and treated with 10 μМ Niraparib, 100 μМ SSO (sulfosuccinimidyl oleate), or a combination for 72 h. Representative C1 BODIPY 500/510C12 staining images were shown. Scale bar=50 μm. The intensity of C1 BODIPY 500/510C12 staining was assessed by Image Pro Plus 6.0.
Sulfosuccinimidyl oleate sodium purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar:80:103528. [Abstract]
OVCAR8 cells were cultured in fatty acid-free media supplemented with 1.0 μL/mL exogenous fatty acids and treated with 10 μМ Niraparib, 100 μМ SSO (sulfosuccinimidyl oleate), or a combination for 72 h. The ROS levels were measured by flow cytometry.
Sulfosuccinimidyl oleate sodium purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar:80:103528. [Abstract]
Tp53-deficient ID8 tumor-bearing mice were administered with Niraparib (50 mg/kg) from day 6, and administered with Lipro-1 (10 mg/kg, intraperitoneally, a single dose) and SSO (sulfosuccinimidyl oleate) (50 mg/kg, intragastrically, a single dose) from day 3. Representative actual or bioluminescent images of mice after a 3-week treatment.
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Adv Sci (Weinh)
CEBPG-Mediated Palmitic Acid Adaptation of Cancer-Associated Fibroblasts Drives Metastasis of Oral Squamous Cell Carcinoma. [Abstract]2026 Jun 2:e75875. PMID: 42227941 -
Cell Rep Med
A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML. [Abstract]2024 Jun 18;5(6):101592. PMID: 38843841
Sulfosuccinimidyl oleate sodium purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2024 Jun 18;5(6):101592. [Abstract]
AML cells labeled with a chemical probe (alk-C16) for 4 h in the presence of Sulfosuccinimidyl oleate (SSO) (50 μM) were used for palmitoylation assay.
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Cell Death Dis
A targetable antioxidant defense mechanism to EZH2 inhibitors enhances tumor cell vulnerability to ferroptosis. [Abstract]2025 Apr 14;16(1):291. PMID: 40229247 -
Cell Death Dis
TREM2-dependent activation of microglial cell protects photoreceptor cell during retinal degeneration via PPARγ and CD36. [Abstract]2024 Aug 26;15(8):623. PMID: 39187498
Sulfosuccinimidyl oleate sodium purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Aug 26;15(8):623. [Abstract]
Quantitative analysis of the outer nuclear layer thickness in SSO (sulfosuccinimidyl oleate) (50 mg/kg, oral gavage)-treated MNU-induced WT mouse retina and GW1929 (30 mg/kg, intraperitoneal injection, twice)+SSO treated MNU-induced Trem2−/− mouse retina.
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Cancer Lett
PA suppresses antitumor immunity of T cells by disturbing mitochondrial activity through Akt/mTOR-mediated Ca2+ flux. [Abstract]2024 Jan 28:581:216511. PMID: 38013049 -
J Exp Med
Repurposing a tricyclic antidepressant in tumor and metabolism disease treatment through fatty acid uptake inhibition. [Abstract]2023 Mar 6;220(3):e20221316. PMID: 36520461 -
J Transl Med
Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice. [Abstract]2023 Feb 6;21(1):89. PMID: 36747238 -
Food Res Int
Hydroxypropyl-β-cyclodextrin as a co-delivery vehicle for synergistically enhancing fucoxanthin oral bioavailability through dual regulation of SR-B1 and ABCB1 transporters. [Abstract]2026 Mar 31:228:118352. PMID: 41703824 -
Food Res Int
Cluster-determinant 36 (CD36) mediates intestinal absorption of dietary astaxanthin and affects its secretion. [Abstract]2023 Nov;173(Pt 1):113328. PMID: 37803639 -
Int J Biol Macromol
Stearic acid promotes lipid synthesis through CD36/Fyn/FAK/mTORC1 axis in bovine mammary epithelial cells. [Abstract]2023 Dec 31;253(Pt 8):127324. PMID: 37838116 -
Cell Rep
Tfam-mediated metabolic perturbation in RORγt+ lymphocytes impacts intestinal tissue homeostasis and promotes GATA3+RORγt+ innate lymphoid cells. [Abstract]2026 Feb 24;45(2):116952. PMID: 41678335 -
Cell Rep
CD36-mediated arachidonic acid influx from decidual stromal cells increases inflammatory macrophages in miscarriage. [Abstract]2024 Oct 19;43(11):114881. PMID: 39427314 -
Cell Rep
Excessive free fatty acid sensing in pituitary lactotrophs elicits steatotic liver disease by decreasing prolactin levels. [Abstract]2024 Jul 9;43(7):114465. PMID: 38985678 -
J Invest Dermatol
CD36-mediated long-chain fatty acids transport in keratinocytes promotes psoriasis-like skin inflammation by increasing mitochondrial ROS. [Abstract]2025 Nov 20:S0022-202X(25)03555-9. PMID: 41274545 -
Cancer Cell Int
BCR::ABL1 expression in chronic myeloid leukemia cells in low oxygen is regulated by glutamine via CD36-mediated fatty acid uptake. [Abstract]2025 May 14;25(1):176. PMID: 40369538 -
JCI Insight
Complement activation at the interface between adipocytes and cancer cells drives tumor progression. [Abstract]2025 Feb 18;10(6):e184935. PMID: 39964754 -
J Agric Food Chem
Intestinal Absorption of Dietary Astaxanthin Mediated by Cluster-Determinant 36 (CD36) Presents Stereoisomeric Selectivity In Vitro and In Vivo. [Abstract]2026 Feb 11;74(5):4407-4419. PMID: 41631482 -
J Agric Food Chem
Cluster of Differentiation 36 (CD36) Preferentially Mediates Intestinal Absorption of Dietary Z-Astaxanthin and Especially 9- Z-Isomer via Higher Binding Affinity. [Abstract]2024 Jul 24;72(29):16287-16297. PMID: 38986018 -
CNS Neurosci Ther
Tongqiao Huoxue Decoction ameliorates traumatic brain injury-induced gastrointestinal dysfunction by regulating CD36/15-LO/NR4A1 signaling, which fails when CD36 and CX3CR1 are deficient. [Abstract]2023 Jun;29 Suppl 1(Suppl 1):161-184. PMID: 37157929 -
Cancer Metab
CD36 inhibition enhances the anti-proliferative effects of PI3K inhibitors in PTEN-loss anti-HER2 resistant breast cancer cells. [Abstract]2025 Feb 7;13(1):6. PMID: 39920872 -
Nutrients
Medium-Chain Fatty Acids Selectively Sensitize Cancer Cells to Ferroptosis by Inducing CD36 and ACSL4. [Abstract]2025 Feb 25;17(5):794. PMID: 40077664 -
Int Immunopharmacol
Aloe-emodin promotes remyelination by driving microglial myelin debris clearance via the CD36-PPARγ axis. [Abstract]2026 Apr 27:181:116718. PMID: 42048733 -
Int Immunopharmacol
Rutin ameliorates hepatic ischemia-reperfusion injury by targeting CD36 to suppress hepatocyte ferroptosis. [Abstract]2026 Mar 1:172:116231. PMID: 41576568 -
Int Immunopharmacol
CD36-mediated fatty acid metabolic reprogramming activates synovial fibroblasts and promotes rheumatoid arthritis progression. [Abstract]2025 Dec 10:167:115594. PMID: 41101221 -
Int J Mol Sci
CD36 Induces Inflammation by Promoting Ferroptosis in Pancreas, Epididymal Adipose Tissue, and Adipose Tissue Macrophages in Obesity-Related Severe Acute Pancreatitis. [Abstract]2025 Apr 8;26(8):3482. PMID: 40331957 -
Int J Mol Sci
Chlorogenic Acid Alleviates LPS-Induced Inflammation and Oxidative Stress by Modulating CD36/AMPK/PGC-1α in RAW264.7 Macrophages. [Abstract]2023 Aug 31;24(17):13516. PMID: 37686324 -
Front Pharmacol
Carnosine Stimulates Macrophage-Mediated Clearance of Senescent Skin Cells Through Activation of the AKT2 Signaling Pathway by CD36 and RAGE. [Abstract]2020 Dec 16:11:593832. PMID: 33390976 -
Toxicol Sci
2,3,7,8-Tetrachlorodibenzo-dioxin induces liver lipid metabolism disorder via the ROS/AMPK/CD36 signaling pathway. [Abstract]2023 Feb 17;191(2):276-284. PMID: 36534932 -
Food Sci Nutr
The effect of in vitro digestion on the interaction between polysaccharides derived from Pleurotus eryngii and intestinal mucus. [Abstract]2023 Nov 22;12(2):1318-1329. PMID: 38370047 -
BMC Cancer
Tumor-infiltrating CD36+CD8+T cells determine exhausted tumor microenvironment and correlate with inferior response to chemotherapy in non-small cell lung cancer. [Abstract]2023 Apr 21;23(1):367. PMID: 37085798 -
Toxins
2021 Nov 13;13(11):802. PMID: 34822586 -
Cell Immunol
2022 Feb:372:104475. PMID: 35063904 -
Fish Physiol Biochem
Central administration of docosahexaenoic acid, eicosapentaenoic acid, or linoleic acid activates hypothalamic fatty acid sensing and is involved in reproductive regulation in spotted scat (Scatophagus argus). [Abstract]2025 Nov 24;51(6):197. PMID: 41284092 -
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Heliyon
CB2R activation enhances tumor-associated macrophages-mediated phagocytosis of glioma cell. [Abstract]2024 Nov 28;10(23):e40806. PMID: 39691192 -
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Solvent & Solubility
DMSO : 62.5 mg/mL (129.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 3.33 mg/mL (6.91 mM); Suspended solution; Need ultrasonic
Add each solvent one by one: 0.5% Methylcellulose/saline water
Solubility: 3.33 mg/mL (6.91 mM); Suspended solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Dhungana H, et al. Sulfosuccinimidyl oleate sodium is neuroprotective and alleviates stroke-induced neuroinflammation. J Neuroinflammation. 2017 Dec 4;14(1):237. [Content Brief]
[2]. Drahota Z, et al. Succinimidyl oleate, established inhibitor of CD36/FAT translocase inhibits complex III of mitochondrial respiratory chain. Biochem Biophys Res Commun. 2010 Jan 15;391(3):1348-51. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0765 mL | 10.3825 mL | 20.7650 mL | 51.9125 mL |
| 5 mM | 0.4153 mL | 2.0765 mL | 4.1530 mL | 10.3825 mL | |
| 10 mM | 0.2076 mL | 1.0382 mL | 2.0765 mL | 5.1912 mL | |
| 15 mM | 0.1384 mL | 0.6922 mL | 1.3843 mL | 3.4608 mL | |
| 20 mM | 0.1038 mL | 0.5191 mL | 1.0382 mL | 2.5956 mL | |
| 25 mM | 0.0831 mL | 0.4153 mL | 0.8306 mL | 2.0765 mL | |
| 30 mM | 0.0692 mL | 0.3461 mL | 0.6922 mL | 1.7304 mL | |
| 40 mM | 0.0519 mL | 0.2596 mL | 0.5191 mL | 1.2978 mL | |
| 50 mM | 0.0415 mL | 0.2076 mL | 0.4153 mL | 1.0382 mL | |
| 60 mM | 0.0346 mL | 0.1730 mL | 0.3461 mL | 0.8652 mL | |
| 80 mM | 0.0260 mL | 0.1298 mL | 0.2596 mL | 0.6489 mL | |
| 100 mM | 0.0208 mL | 0.1038 mL | 0.2076 mL | 0.5191 mL |