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  4. Sulfosuccinimidyl oleate sodium

Sulfosuccinimidyl oleate sodium  (Synonyms: Sulfo-N-succinimidyl oleate sodium)

Cat. No.: HY-112847A Pureza: 99.66%
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SDS
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Sulfosuccinimidyl oleate sodium (Sulfo-N-succinimidyl oleate sodium) is a long chain fatty acid that inhibits fatty acid transport into cells. Sulfosuccinimidyl oleate sodium is a potent and irreversible inhibitor of mitochondrial respiratory chain. Sulfosuccinimidyl oleate sodium binds the CD36 receptor on the surface of microglia. Anti-inflammatory effect.

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
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Revisión del cliente

Based on 39 publication(s) in Google Scholar

Other Forms of Sulfosuccinimidyl oleate sodium:

Sulfosuccinimidyl oleate sodium Related Antibodies

Top Publications Citing Use of Products

39 Publications Citing Use of MCE Sulfosuccinimidyl oleate sodium

WB
In Vivo Efficacy Study
IF
Cell Imaging/Staining
In Vivo Imaging

    Sulfosuccinimidyl oleate sodium purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Feb 3:80:103528.  [Abstract]

    OVCAR8 cells were cultured in fatty acid-free media supplemented with 1.0 μL/mL exogenous fatty acids and treated with 10 μМ Niraparib, 100 μМ SSO (sulfosuccinimidyl oleate), or a combination for 72 h. Representative C1 BODIPY 500/510C12 staining images were shown. Scale bar=50 μm. The intensity of C1 BODIPY 500/510C12 staining was assessed by Image Pro Plus 6.0.

    Sulfosuccinimidyl oleate sodium purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Feb 3:80:103528.  [Abstract]

    OVCAR8 cells were cultured in fatty acid-free media supplemented with 1.0 μL/mL exogenous fatty acids and treated with 10 μМ Niraparib, 100 μМ SSO (sulfosuccinimidyl oleate), or a combination for 72 h. The ROS levels were measured by flow cytometry.

    Sulfosuccinimidyl oleate sodium purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Feb 3:80:103528.  [Abstract]

    Tp53-deficient ID8 tumor-bearing mice were administered with Niraparib (50 mg/kg) from day 6, and administered with Lipro-1 (10 mg/kg, intraperitoneally, a single dose) and SSO (sulfosuccinimidyl oleate) (50 mg/kg, intragastrically, a single dose) from day 3. Representative actual or bioluminescent images of mice after a 3-week treatment.

    Sulfosuccinimidyl oleate sodium purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2024 May 29:101592.  [Abstract]

    AML cells labeled with a chemical probe (alk-C16) for 4 h in the presence of Sulfosuccinimidyl oleate (SSO) (50 μM) were used for palmitoylation assay.

    Sulfosuccinimidyl oleate sodium purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Aug 26;15(8):623.  [Abstract]

    Quantitative analysis of the outer nuclear layer thickness in SSO (sulfosuccinimidyl oleate) (50 mg/kg, oral gavage)-treated MNU-induced WT mouse retina and GW1929 (30 mg/kg, intraperitoneal injection, twice)+SSO treated MNU-induced Trem2−/− mouse retina.

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    Descripciòn

    Sulfosuccinimidyl oleate sodium (Sulfo-N-succinimidyl oleate sodium) is a long chain fatty acid that inhibits fatty acid transport into cells. Sulfosuccinimidyl oleate sodium is a potent and irreversible inhibitor of mitochondrial respiratory chain. Sulfosuccinimidyl oleate sodium binds the CD36 receptor on the surface of microglia. Anti-inflammatory effect[1][2].

    In Vitro

    Sulfosuccinimidyl oleate (20 μM and 50 μM, 24 hours) alone does not alter the cellular viability. Exposure to 100 ng/ml LPS+5 ng/mL IFNγ modestly, yet significantly reduces the viability of the BV2 cells. Co-treatment with Sulfosuccinimidyl oleate prevents the LPS+IFNγ-induced reduction in the cell viability[1].
    Sulfosuccinimidyl oleate (50 μM, 24 hours) co-treatment significantly reduces the LPS+IFNγ-induced expression of NOS2 and COX-2 in BV2 cells. Western blot analysis reveals a significant LPS/IFNγ-induced upregulation in the phosphorylated form of the p38, which is prevented by co-treatment with Sulfosuccinimidyl oleate (50 μM, 24 hours)[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Sulfosuccinimidyl oleate sodium Related Antibodies

    Cell Viability Assay[1]

    Cell Line: BV2 cells
    Concentration: 20 μM and 50 μM
    Incubation Time: 24 hours
    Result: Did not alter the viability of BV2 cells alone. Exposure of BV2 cells to 100 ng/mL LPS and 5 ng/mL IFNγ significantly reduced the viability of BV2 cells while simultaneous treatment with Sulfosuccinimidyl oleate prevented it.

    Western Blot Analysis[1]

    Cell Line: BV2 cells
    Concentration: 50 μM
    Incubation Time: 24 hours
    Result: Drastically increased the levels of NOS2, COX-2, and P-p38/T-p38.
    In Vivo

    the Sulfosuccinimidyl oleate (50 mg/kg; administered once by single oral gavage) significantly reduces the cortical ischemic infarct size compared to vehicle-treated controls in male BALB/cABom mice with pMCAo model. In addition, Sulfosuccinimidyl oleate at 50 mg/kg is suitable to see a beneficial effect after stroke[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 4-month-old male BALB/cABom mice with pMCAo model[1]
    Dosage: 50 mg/kg
    Administration: Administered once by single oral gavage
    Result: Reduced brain damage following ischemia. Attenuated infarct size.
    Peso molecular

    481.58

    Fòrmula

    C22H36NNaO7S
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=S(C(C1)C(N(OC(CCCCCCC/C=C\CCCCCCCC)=O)C1=O)=O)(O[Na])=O
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvente y solubilidad
    In Vitro: 

    DMSO : 62.5 mg/mL (129.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0765 mL 10.3825 mL 20.7650 mL
    5 mM 0.4153 mL 2.0765 mL 4.1530 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.32 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (4.32 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 3.33 mg/mL (6.91 mM); Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  0.5% Methylcellulose/saline water

      Solubility: 3.33 mg/mL (6.91 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Pureza y Documentación

    Purity: 99.66%

    SDS (393 KB)

    COA (241 KB) HNMR (143 KB) RP-HPLC (250 KB) LCMS (239 KB)

    Instrucciones de manejo (2659 KB)
    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.0765 mL 10.3825 mL 20.7650 mL 51.9125 mL
    5 mM 0.4153 mL 2.0765 mL 4.1530 mL 10.3825 mL
    10 mM 0.2076 mL 1.0382 mL 2.0765 mL 5.1912 mL
    15 mM 0.1384 mL 0.6922 mL 1.3843 mL 3.4608 mL
    20 mM 0.1038 mL 0.5191 mL 1.0382 mL 2.5956 mL
    25 mM 0.0831 mL 0.4153 mL 0.8306 mL 2.0765 mL
    30 mM 0.0692 mL 0.3461 mL 0.6922 mL 1.7304 mL
    40 mM 0.0519 mL 0.2596 mL 0.5191 mL 1.2978 mL
    50 mM 0.0415 mL 0.2076 mL 0.4153 mL 1.0382 mL
    60 mM 0.0346 mL 0.1730 mL 0.3461 mL 0.8652 mL
    80 mM 0.0260 mL 0.1298 mL 0.2596 mL 0.6489 mL
    100 mM 0.0208 mL 0.1038 mL 0.2076 mL 0.5191 mL
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    Sulfosuccinimidyl oleate sodium Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Sulfosuccinimidyl oleateSulfo-N-succinimidyl oleateMitophagyMitochondrial AutophagyInhibitorinhibitorinhibit

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    Nombre del producto:
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