2. Autophagy
  3. Mitophagy
  4. Sulfosuccinimidyl oleate

Sulfosuccinimidyl oleate  (Synonyms: Sulfo-N-succinimidyl oleate)

Cat. No.: HY-112847
Handling Instructions Technical Support

Sulfosuccinimidyl oleate (Sulfo-N-succinimidyl oleate) is a long chain fatty acid that inhibits fatty acid transport into cells. Sulfosuccinimidyl oleate is a potent and irreversible inhibitor of mitochondrial respiratory chain. Sulfosuccinimidyl oleate binds the CD36 receptor on the surface of microglia. Anti-inflammatory effect.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (Sulfosuccinimidyl oleate sodium) that retains the same biological activity.

For research use only. We do not sell to patients.

CAS No. : 135661-44-8

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Sulfosuccinimidyl oleate Related Antibodies

Top Publications Citing Use of Products

39 Publications Citing Use of MCE Sulfosuccinimidyl oleate

WB
In Vivo Efficacy Study
IF
Cell Imaging/Staining
In Vivo Imaging

    Sulfosuccinimidyl oleate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Feb 3:80:103528.  [Abstract]

    OVCAR8 cells were cultured in fatty acid-free media supplemented with 1.0 μL/mL exogenous fatty acids and treated with 10 μМ Niraparib, 100 μМ SSO (sulfosuccinimidyl oleate), or a combination for 72 h. Representative C1 BODIPY 500/510C12 staining images were shown. Scale bar=50 μm. The intensity of C1 BODIPY 500/510C12 staining was assessed by Image Pro Plus 6.0.

    Sulfosuccinimidyl oleate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Feb 3:80:103528.  [Abstract]

    OVCAR8 cells were cultured in fatty acid-free media supplemented with 1.0 μL/mL exogenous fatty acids and treated with 10 μМ Niraparib, 100 μМ SSO (sulfosuccinimidyl oleate), or a combination for 72 h. The ROS levels were measured by flow cytometry.

    Sulfosuccinimidyl oleate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Feb 3:80:103528.  [Abstract]

    Tp53-deficient ID8 tumor-bearing mice were administered with Niraparib (50 mg/kg) from day 6, and administered with Lipro-1 (10 mg/kg, intraperitoneally, a single dose) and SSO (sulfosuccinimidyl oleate) (50 mg/kg, intragastrically, a single dose) from day 3. Representative actual or bioluminescent images of mice after a 3-week treatment.

    Sulfosuccinimidyl oleate purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2024 May 29:101592.  [Abstract]

    AML cells labeled with a chemical probe (alk-C16) for 4 h in the presence of Sulfosuccinimidyl oleate (SSO) (50 μM) were used for palmitoylation assay.

    Sulfosuccinimidyl oleate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Aug 26;15(8):623.  [Abstract]

    Quantitative analysis of the outer nuclear layer thickness in SSO (sulfosuccinimidyl oleate) (50 mg/kg, oral gavage)-treated MNU-induced WT mouse retina and GW1929 (30 mg/kg, intraperitoneal injection, twice)+SSO treated MNU-induced Trem2−/− mouse retina.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Sulfosuccinimidyl oleate (Sulfo-N-succinimidyl oleate) is a long chain fatty acid that inhibits fatty acid transport into cells. Sulfosuccinimidyl oleate is a potent and irreversible inhibitor of mitochondrial respiratory chain. Sulfosuccinimidyl oleate binds the CD36 receptor on the surface of microglia. Anti-inflammatory effect[1][2].

    In Vitro

    Sulfosuccinimidyl oleate (20 μM and 50 μM, 24 hours) alone does not alter the cellular viability. Exposure to 100 ng/ml LPS+5 ng/mL IFNγ modestly, yet significantly reduces the viability of the BV2 cells. Co-treatment with Sulfosuccinimidyl oleate prevents the LPS+IFNγ-induced reduction in the cell viability[1].
    Sulfosuccinimidyl oleate (50 μM, 24 hours) co-treatment significantly reduces the LPS+IFNγ-induced expression of NOS2 and COX-2 in BV2 cells. Western blot analysis reveals a significant LPS/IFNγ-induced upregulation in the phosphorylated form of the p38, which is prevented by co-treatment with Sulfosuccinimidyl oleate (50 μM, 24 hours)[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Sulfosuccinimidyl oleate Related Antibodies

    Cell Viability Assay[1]

    Cell Line: BV2 cells
    Concentration: 20 μM and 50 μM
    Incubation Time: 24 hours
    Result: Did not alter the viability of BV2 cells alone. Exposure of BV2 cells to 100 ng/mL LPS and 5 ng/mL IFNγ significantly reduced the viability of BV2 cells while simultaneous treatment with Sulfosuccinimidyl oleate prevented it.

    Western Blot Analysis[1]

    Cell Line: BV2 cells
    Concentration: 50 μM
    Incubation Time: 24 hours
    Result: Drastically increased the levels of NOS2, COX-2, and P-p38/T-p38.
    In Vivo

    Sulfosuccinimidyl oleate (50 mg/kg; administered once by single oral gavage) significantly reduces the cortical ischemic infarct size compared to vehicle-treated controls in male BALB/cABom mice with pMCAo model. In addition, Sulfosuccinimidyl oleate at 50 mg/kg is suitable to see a beneficial effect after stroke[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 4-month-old male BALB/cABom mice with pMCAo model[1]
    Dosage: 50 mg/kg
    Administration: Administered once by single oral gavage
    Result: Reduced brain damage following ischemia. Attenuated infarct size.
    Molecular Weight

    459.60

    Formula

    C22H37NO7S
    CAS No.
    SMILES

    O=S(C(C1)C(N(OC(CCCCCCC/C=C\CCCCCCCC)=O)C1=O)=O)(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Sulfosuccinimidyl oleate Related Classifications

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Sulfosuccinimidyl oleate135661-44-8Sulfo-N-succinimidyl oleateMitophagyMitochondrial AutophagyInhibitorinhibitorinhibit

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