T4 UvsX Recombinase 

T4 UvsX Recombinase helps initiate DNA replication on a double-stranded DNA template by catalyzing synapsis between the template and a homologous DNA single strand that serves as primer. T4 UvsX Recombinase greatly amplifies the snap-back (hairpin-primed) DNA synthesis that is catalyzed by the T4 DNA polymerase holoenzyme on linear, single-stranded DNA templates^[1].

Product Information
T4 UvsX recombinase is derived from T4 bacteriophage and is a homolog of the RecA/Rad51 family. It plays an important role in the repair of double-strand DNA breaks and the restart of replication forks. It is recombinantly expressed in E.coli and has no nuclease activity. The storage solution is 50 mM Tris-HCl, 50 mM KCI, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.5.

Instructions
Applied to isothermal amplification RPA reactions, and the recommended addition amount is 300-400 ng/μL.

Precautions
Avoid repeated freezing and thawing.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Liquid

Colorless to light yellow

[T4 UvsX Recombinase]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Morrical SW, et al. Amplification of snap-back DNA synthesis reactions by the uvsX recombinase of bacteriophage T4. J Biol Chem. 1991 Jul 25;266(21):14031-8.  [Content Brief]