URAT1 inhibitor 1

Name: URAT1 inhibitor 1
Brand: MedChemExpress
SKU: HY-114309
Rating: 4.5 (0 reviews)

Cat. No.: HY-114309 Pureté: 97.08%: Fiche technique
COA

SDS
Instruction de manipulation
FAQs
Technical Support

URAT1 inhibitor 1 (1g) is a uric acid transporter 1 (URAT1) inhibitor, with an IC₅₀ of 32 nM. URAT1 inhibitor 1 has potential to treat hyperuricemia associated with gout.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

URAT1 inhibitor 1 Chemical Structure

CAS No. : 2268720-62-1

Size	Stock	Quantité
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtenir un devis
500 mg	Obtenir un devis

or Bulk Inquiry

* Veuillez sélectionner la quantité avant d'ajouter des articles.

This product is a controlled substance and not for sale in your territory.

Avis client

Based on 1 Customer Validation

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Activité biologique
Pureté et documentation
Références
Avis client

Description

URAT1 inhibitor 1 (1g) is a uric acid transporter 1 (URAT1) inhibitor, with an IC₅₀ of 32 nM. URAT1 inhibitor 1 has potential to treat hyperuricemia associated with gout^[1].

IC₅₀ & Target

IC50: 32 nM (URAT1)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	0.032 3 Compound: 1g	Inhibition of human URAT1 expressed in HEK293 cells assessed as reduction in [8-14C]uric acid uptake Inhibition of human URAT1 expressed in HEK293 cells assessed as reduction in [8-14C]uric acid uptake	[PMID: 30579795]
HEK293	IC₅₀	0.032 3 Compound: 1g	Inhibition of human URAT1 expressed in HEK293 cells assessed as reduction in [8-14C]uric acid uptake Inhibition of human URAT1 expressed in HEK293 cells assessed as reduction in [8-14C]uric acid uptake	[PMID: 30579795]

Masse moléculaire

569.29

Formule

C₁₉H₁₅Br₂N₅O₂S₂

CAS No.

2268720-62-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

BrC1=CC=C(CN2C(SCS(NC3=CC=CN=C3)(=O)=O)=NN=C2Br)C4=C1C=CC=C4

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvant et solubilité

In Vitro:

DMSO : 125 mg/mL (219.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7566 mL	8.7829 mL	17.5657 mL
5 mM	0.3513 mL	1.7566 mL	3.5131 mL
10 mM	0.1757 mL	0.8783 mL	1.7566 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 97.08%

Select Batch:

Fiche technique (275 KB) SDS (251 KB)

COA (253 KB) HNMR (286 KB) LCMS (261 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Wu JW, et al. Synthesis, biological evaluation and 3D-QSAR studies of 1,2,4-triazole-5-substituted carboxylic acid bioisosteres as uric acid transporter 1 (URAT1) inhibitors for the treatment of hyperuricemia associated with gout. Bioorg Med Chem Lett. 20 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7566 mL	8.7829 mL	17.5657 mL	43.9144 mL
	5 mM	0.3513 mL	1.7566 mL	3.5131 mL	8.7829 mL
	10 mM	0.1757 mL	0.8783 mL	1.7566 mL	4.3914 mL
	15 mM	0.1171 mL	0.5855 mL	1.1710 mL	2.9276 mL
	20 mM	0.0878 mL	0.4391 mL	0.8783 mL	2.1957 mL
	25 mM	0.0703 mL	0.3513 mL	0.7026 mL	1.7566 mL
	30 mM	0.0586 mL	0.2928 mL	0.5855 mL	1.4638 mL
	40 mM	0.0439 mL	0.2196 mL	0.4391 mL	1.0979 mL
	50 mM	0.0351 mL	0.1757 mL	0.3513 mL	0.8783 mL
	60 mM	0.0293 mL	0.1464 mL	0.2928 mL	0.7319 mL
	80 mM	0.0220 mL	0.1098 mL	0.2196 mL	0.5489 mL
	100 mM	0.0176 mL	0.0878 mL	0.1757 mL	0.4391 mL