WRW4

Name: WRW4
Brand: MedChemExpress
SKU: HY-P1119
Rating: 5.0 (11 reviews)

Cat. No.: HY-P1119 Purity: 99.80%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

WRW4, a specific formyl peptide receptor-like 1 (FPRL1) antagonist, inhibits WKYMVm binding to FPRL1 with an IC₅₀ of 0.23 μM. WRW4 specifically inhibits the increase in intracellular calcium by the FPRL1 agonists MMK-1, amyloid beta42 (Abeta42) peptide, and F peptide.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 878557-55-2

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of WRW4:

WRW4 TFA Get quote

11 Publications Citing Use of MCE WRW4

Histological Imaging/Staining

IHC

ELISA

: WRW4 purchased from MedChemExpress. Usage Cited in: Cell Mol Biol Lett. 2023 Jan 19;28(1):4. [Abstract]; Inhibition of elevated FPR2 is protective against kidney injury. The left renal pedicle of the rat was clamped for 45 min to induce IRI. The right kidney was removed before releasing the clamps. WRW4 (10 mg/kg; single dose) was administered intravenously 30 min before surgery. Serum or kidney tissues were collected 24 h after surgery. Kidney tissues were sectioned for histological examination and TUNEL staining. Scale bar, 100 μm. TUNEL staining results showed a decrease in the number of apoptotic renal tubular cells in the WRW4 pretreatment group compared with the surgical group.

: WRW4 purchased from MedChemExpress. Usage Cited in: Cell Mol Biol Lett. 2023 Jan 19;28(1):4. [Abstract]; Inhibition of FPR2 reduces neutrophil infiltration in IRI kidneys. The left renal pedicle of the rat was clamped for 45 min to induce IRI. The right kidney was removed before releasing the clamps. WRW4 (10 mg/kg; single dose) was administered intravenously 30 min before surgery. Kidney tissues were collected 24 h after surgery. A Immunochemistry staining of CD68⁺ macrophages and MPO⁺ neutrophils. Scale bar, 100 μm.

: WRW4 purchased from MedChemExpress. Usage Cited in: Cancer Res. 2022 Aug 16;82(16):2887-2903. [Abstract]; Detection of PLD activity and PA production in the indicated cells. Cells were treated with cyclosporine H (5 μmol/L; FPR1 inhibitor) or WRW4 (10 μmol/L; FPR2 inhibitor) for 1 hour. The fold increases after D-AS2 overexpression and fold decreases after D-AS2 depletion are shown.

: WRW4 purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2022 Aug 31;70(34):10532-10542. [Abstract]; Positive expression of p65 in liver tissue was observed by immunofluorescence staining under a laser scanning confocal microscope. The p65 cells were marked in red, and the nucleus was stained with 4′,6-diamidino-2-phenylindole (DAPI) in blue. Scale bars = 50 μm. The results showed that the expression level of p65 within the hepatocytes' nuclei was elevated in the CRS group rats; in the CRS + CGA group, the p65 expression level was reduced; furthermore, this effect of CGA was reversed by WRW4 (0.1 mg/kg; i.p.).

: WRW4 purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2022 Aug 31;70(34):10532-10542. [Abstract]; Levels of serum RvD1 and RvD1 in liver tissue. ELISA results showed that RvD1 levels in serum and liver were significantly lower in CRS rats than in CON rats, and significantly higher in CGA-treated rats; this effect was prevented by WRW4 (0.1 mg/kg; i.p.).

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Description

In Vitro

WRW4 inhibits Abeta42 peptide-induced superoxide generation and chemotactic migration of neutrophils, and also completely inhibits the internalization of Abeta42 peptide in human macrophages. WRW4 specifically blocks ERK phosphorylation downstream of FPRL1 by WKYMVm^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1104.27

Formula

C₆₁H₆₅N₁₅O₆

CAS No.

878557-55-2

Appearance

Solid

Color

White to off-white

Sequence

Trp-Arg-Trp-Trp-Trp-Trp-NH2

Sequence Shortening

WRWWWW-NH2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (90.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9056 mL	4.5279 mL	9.0558 mL
5 mM	0.1811 mL	0.9056 mL	1.8112 mL
10 mM	0.0906 mL	0.4528 mL	0.9056 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (2.26 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (2.26 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light, under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (256 KB) LCMS (108 KB)

Handling Instructions (2659 KB)

References

[1]. Bae YS, et al. Identification of peptides that antagonize formyl peptide receptor-like 1-mediated signaling. J Immunol. 2004;173(1):607-614. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.9056 mL	4.5279 mL	9.0558 mL	22.6394 mL
	5 mM	0.1811 mL	0.9056 mL	1.8112 mL	4.5279 mL
	10 mM	0.0906 mL	0.4528 mL	0.9056 mL	2.2639 mL
	15 mM	0.0604 mL	0.3019 mL	0.6037 mL	1.5093 mL
	20 mM	0.0453 mL	0.2264 mL	0.4528 mL	1.1320 mL
	25 mM	0.0362 mL	0.1811 mL	0.3622 mL	0.9056 mL
	30 mM	0.0302 mL	0.1509 mL	0.3019 mL	0.7546 mL
	40 mM	0.0226 mL	0.1132 mL	0.2264 mL	0.5660 mL
	50 mM	0.0181 mL	0.0906 mL	0.1811 mL	0.4528 mL
	60 mM	0.0151 mL	0.0755 mL	0.1509 mL	0.3773 mL
	80 mM	0.0113 mL	0.0566 mL	0.1132 mL	0.2830 mL

WRW4 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WRW4878557-55-2WRW 4WRW-4Formyl Peptide Receptor (FPR)Abeta42superoxidegenerationneutrophilsmigrationmacrophagesphosphorylationInhibitorinhibitorinhibit

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WRW4

Customer Review

Other Forms of WRW4:

WRW4 Related Antibodies

11 Publications Citing Use of MCE WRW4

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•Related Screening Libraries:

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Biological Activity

Purity & Documentation

References

Customer Review

WRW4 Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

WRW4 Related Classifications

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