1-Naphthyl acetate

Name: 1-Naphthyl acetate
Brand: MedChemExpress
SKU: HY-W016188
Rating: 4.5 (0 reviews)

Cat. No.: HY-W016188 Pureza: 99.89%: Ficha de datos
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SDS
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1-Naphthyl acetate is an attractive chromogenic substrate for the detection of erythrocyte acetylcholinesterase (AChE) activity. 1-Naphthyl acetate has the potential to detect organophosphorus pesticide (OP) poisoning.

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1-Naphthyl acetate Estructura química

No. CAS : 830-81-9

Tamaño	Stock	Cantidad

Muestra gratis (0.1 - 0.2 mg)	Solicite ahora
Muestra gratis (0.1 - 0.2 mg)	Solicite ahora
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
1 g	En stock	Estimated Time of Arrival: December 31
5 g	En stock	Estimated Time of Arrival: December 31
10 g	En stock	Estimated Time of Arrival: December 31
25 g	En stock	Estimated Time of Arrival: December 31
50 g	En stock	Estimated Time of Arrival: December 31
100 g	En stock	Estimated Time of Arrival: December 31
> 100 g	Obtener un presupuesto

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Based on 1 Customer Validation

Other Forms of 1-Naphthyl acetate:

1-Naphthyl acetate (Standard) Obtener un presupuesto

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Cholinesterase (ChE):

Ver todas las isoformas

AChE BChE

Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

IC₅₀ & Target

AChE

In Vitro

1-Naphthyl acetate can be hydrolyzed by AChE into 1-Naphthol (HY-Y1309) and Acetic acid (HY-Y0319). 1-Naphthol can react with colorimetric reagents (such as Fast Blue RR (HY-W127771)) to generate colored products, which can be used for colorimetric analysis to quantitatively measure the activity of AChE^[1].
1-Naphthyl acetate be used for quick detection of organophosphorus pesticide (OP) poisoning, that OP inhibits the activity of AChE, resulting in reduced hydrolysis of 1-Naphthyl acetate^[1].
1-Naphthyl acetate can be hydrolyzed by intracellular esterases in plants cells to release acetic acid and 1-naphthol, leading to acidification of the extracellular medium. Acidification of the extracellular medium is considered to be one of the key factors in cell elongation. The acidified medium activates the proton pump on the cytoplasmic membrane, thereby promoting proton efflux and further reducing the cytoplasmic pH. The reduced cytoplasmic pH can activate the expansins in the cell wall, leading to cell wall relaxation, thereby promoting cell elongation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

186.21

Fòrmula

C₁₂H₁₀O₂

No. CAS

830-81-9

Appearance

Solid

Color

Off-white to light yellow

SMILES

CC(OC1=C2C=CC=CC2=CC=C1)=O

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (537.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.3703 mL	26.8514 mL	53.7028 mL
5 mM	1.0741 mL	5.3703 mL	10.7406 mL
10 mM	0.5370 mL	2.6851 mL	5.3703 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (13.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.89%

Select Batch:

Ficha de datos (276 KB) SDS (476 KB)

COA (253 KB) HNMR (235 KB) LCMS (314 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Chowdhary S, et al. 1-Naphthyl acetate: A chromogenic substrate for the detection of erythrocyte acetylcholinesterase activity. Biochimie. 2018 Nov;154:194-209. [Content Brief]

[2]. Salguero J, et al., 1-Naphthyl Acetate-Dependent Medium Acidification by Zea mays L. Coleoptile Segments. Plant Physiol. 1991 Apr;95(4):1174-80. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.3703 mL	26.8514 mL	53.7028 mL	134.2570 mL
	5 mM	1.0741 mL	5.3703 mL	10.7406 mL	26.8514 mL
	10 mM	0.5370 mL	2.6851 mL	5.3703 mL	13.4257 mL
	15 mM	0.3580 mL	1.7901 mL	3.5802 mL	8.9505 mL
	20 mM	0.2685 mL	1.3426 mL	2.6851 mL	6.7129 mL
	25 mM	0.2148 mL	1.0741 mL	2.1481 mL	5.3703 mL
	30 mM	0.1790 mL	0.8950 mL	1.7901 mL	4.4752 mL
	40 mM	0.1343 mL	0.6713 mL	1.3426 mL	3.3564 mL
	50 mM	0.1074 mL	0.5370 mL	1.0741 mL	2.6851 mL
	60 mM	0.0895 mL	0.4475 mL	0.8950 mL	2.2376 mL
	80 mM	0.0671 mL	0.3356 mL	0.6713 mL	1.6782 mL
	100 mM	0.0537 mL	0.2685 mL	0.5370 mL	1.3426 mL