2,3-Diphospho-D-glyceric acid pentasodium salt  (Synonyms: 2,3-DPG pentasodium salt)

2,3-Diphospho-D-glyceric acid (2,3-DPG) pentasodium salt is a hemoglobin binder and vascular calcification inhibitor that reduces the oxygen affinity of hemoglobin. 2,3-Diphospho-D-glyceric acid pentasodium salt also specifically delays the transformation of colloidal calciprotein particles into crystalline forms, thereby effectively inhibiting vascular smooth muscle cell calcification without affecting the normal formation of osteoid nodules in osteoblast-like cells. 2,3-Diphospho-D-glyceric acid pentasodium salt shows no cytotoxicity to tested cell lines and only weakly interferes with β-hematin formation mediated by glyceryl monopalmitate. 2,3-Diphospho-D-glyceric acid pentasodium salt can be used to study the pathological mechanisms of vascular calcification and malaria-related conditions^[1][2].

2,3-Diphospho-D-glyceric acid pentasodium salt (1-81 μM; 1000 min) dose-dependently delays the conversion of CPPIs to CPPIIs in a cell-free serum-free system, with slightly higher potency than IP6; a concentration of 34.5 μM in human serum and 30.5 μM in fetal bovine serum is required to achieve a T₅₀ of 350 min^[1].
2,3-Diphospho-D-glyceric acid pentasodium salt (100 μM; 7 d) does not impair the proliferation of MC3T3-E1 osteoblastic cells or MOVAS vascular smooth muscle cells; meanwhile, the LDH release is <5% compared with the control^[1].
2,3-Diphospho-D-glyceric acid pentasodium salt (1-100 μM; 72 h) does not alter the morphology of MC3T3-E1 osteoblastic cells or MOVAS vascular smooth muscle cells even at concentrations up to 100 μM following 72 h of incubation^[1].
2,3-Diphospho-D-glyceric acid pentasodium salt (1-16 μM; 7 d) potently inhibits CPPII- and Ca²⁺-induced calcification of MOVAS vascular smooth muscle cells, with an IC₅₀ of 3.8 μM, and achieves complete inhibition at concentrations ≥8 μM after 7 days of incubation^[1].
2,3-Diphospho-D-glyceric acid pentasodium salt (16 μM; 10 d) does not inhibit osteogenic calcification in MC3T3-E1 osteoblastic cells^[1].
2,3-Diphospho-D-glyceric acid pentasodium salt (4.5 mM; 30 min; 37 °C) exerts only a weak effect on β-heme formation mediated by glyceryl monopalmitate, with a final yield of 70.7%^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

375.95

C₃H₃Na₅O₁₀P₂

102783-53-9

Solid

White to light yellow

O=P(O[Na])(O[Na])OC[C@H](C(O[Na])=O)OP(O[Na])(O[Na])=O

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Kharaghani D, et al. 2, 3-Diphospho-D-glyceric acid inhibits calciprotein particle growth and calcification in MOVAS cells but not in MC3T3-E1 cells[J]. Colloid and Interface Science Communications, 2022, 50: 100668.

  [2]. Ambele MA, et al. Neutral lipids associated with haemozoin mediate efficient and rapid β-haematin formation at physiological pH, temperature and ionic composition. Malar J. 2012;11:337. Published 2012 Oct 8.  [Content Brief]