4-Methylumbelliferyl β-D-Glucopyranoside

Name: 4-Methylumbelliferyl β-D-Glucopyranoside
Brand: MedChemExpress
SKU: HY-123633
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Cat. No.: HY-123633 Pureza: 99.87%: Ficha de datos
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4-Methylumbelliferyl β-D-Glucopyranoside, a β-D-glucoside, is a fluorogenic substrate for β-glucosidase, utilizes to assay β-glucosidase activity. 4-Methylumbelliferyl β-D-Glucopyranoside releases the highly fluorescent 4-methylumbelliferyl (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.

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4-Methylumbelliferyl β-D-Glucopyranoside Estructura química

No. CAS : 18997-57-4

Tamaño	Stock	Cantidad

Muestra gratis (0.1 - 0.2 mg)	Solicite ahora
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
500 mg	En stock	Estimated Time of Arrival: December 31
1 g	En stock	Estimated Time of Arrival: December 31
5 g	Obtener un presupuesto
10 g	Obtener un presupuesto

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Based on 1 Customer Validation

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Descripciòn

4-Methylumbelliferyl β-D-Glucopyranoside, a β-D-glucoside, is a fluorogenic substrate for β-glucosidase, utilizes to assay β-glucosidase activity^[1]. 4-Methylumbelliferyl β-D-Glucopyranoside releases the highly fluorescent 4-methylumbelliferyl (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively^[2].

IC₅₀ & Target

β-glucosidase^[1]

In Vitro

The following are the experimental steps for the β-glucosidase GLB1 luciferase activity assay using 4-Methylumbelliferyl β-D-glucopyranoside as a substrate:
1. Reagent preparation
0.64 mM 4-Methylumbelliferyl β-D-glucopyranoside, 50 mM sodium citrate (pH 3.5), recombinant human GLB1 protein expressed in CHO cells.
2. Sample treatment
Incubate at 37°C for 20 minutes and terminate the reaction with alkaline stop solution.
3. Standard curve generation
Generate a standard curve using 0.01-3 nmol/tube of 4-Methylumbelliferone (HY-N0187). The results are linear over the enzyme amount range of 10-300 ng/tube.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

338.31

Fòrmula

C₁₆H₁₈O₈

No. CAS

18997-57-4

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)OC2=CC=C3C(C)=CC(OC3=C2)=O)O)O)O

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (295.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

DMF : 50 mg/mL (147.79 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9559 mL	14.7793 mL	29.5587 mL
5 mM	0.5912 mL	2.9559 mL	5.9117 mL
10 mM	0.2956 mL	1.4779 mL	2.9559 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Pureza y Documentación

Purity: 99.87%

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Ficha de datos (274 KB) SDS (396 KB)

COA (249 KB) HNMR (263 KB) LCMS (96 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Smitka CM,et al. Rapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp. J Clin Microbiol. 1989;27(1):203-206. [Content Brief]

[2]. Oftedal L, et al. Validation and assessment of preanalytical factors of a fluorometric in vitro assay for glucocerebrosidase activity in human cerebrospinal fluid. Sci Rep. 2020;10(1):22098. Published 2020 Dec 16. [Content Brief]

[3]. Boado RJ, et al. Bi-functional IgG-lysosomal enzyme fusion proteins for brain drug delivery. Sci Rep. 2019 Dec 9;9(1):18632. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMF / DMSO	1 mM	2.9559 mL	14.7793 mL	29.5587 mL	73.8967 mL
	5 mM	0.5912 mL	2.9559 mL	5.9117 mL	14.7793 mL
	10 mM	0.2956 mL	1.4779 mL	2.9559 mL	7.3897 mL
	15 mM	0.1971 mL	0.9853 mL	1.9706 mL	4.9264 mL
	20 mM	0.1478 mL	0.7390 mL	1.4779 mL	3.6948 mL
	25 mM	0.1182 mL	0.5912 mL	1.1823 mL	2.9559 mL
	30 mM	0.0985 mL	0.4926 mL	0.9853 mL	2.4632 mL
	40 mM	0.0739 mL	0.3695 mL	0.7390 mL	1.8474 mL
	50 mM	0.0591 mL	0.2956 mL	0.5912 mL	1.4779 mL
	60 mM	0.0493 mL	0.2463 mL	0.4926 mL	1.2316 mL
	80 mM	0.0369 mL	0.1847 mL	0.3695 mL	0.9237 mL
	100 mM	0.0296 mL	0.1478 mL	0.2956 mL	0.7390 mL