2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. AMPPD

AMPPD  (Synonyms: Lumi-Phos Plus; Lumigen PPD; PPD)

Cat. No.: HY-15906 Pureté: 98.29%
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AMPPD (Lumi-Phos Plus; Lumigen PPD) is a chemiluminescent substrate for alkaline phosphatase (APase). AMPPD is hydrolyzed by APase to generate an unstable dioxetane intermediate, and the intermediate releases a chemiluminescent signal when it decomposes. The luminescent signal of AMPPD can be detected by highly sensitive equipment, thereby achieving quantitative analysis of the target molecule. AMPPD can be used in ultrasensitive enzyme-linked immunosorbent assays (such as quantitative detection of human tissue kininogen), chemiluminescent detection of proteins and nucleic acids, and other fields.

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AMPPD

AMPPD Chemical Structure

CAS No. : 122341-56-4

Size Prix Stock Quantité
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in Water En stock
Solid
5 mg En stock
10 mg En stock
25 mg En stock
50 mg En stock
100 mg En stock
200 mg   Obtenir un devis  
500 mg   Obtenir un devis  

* Veuillez sélectionner la quantité avant d'ajouter des articles.

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Based on 1 publication(s) in Google Scholar

AMPPD Related Antibodies

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1 Publications Citing Use of MCE AMPPD

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Description

AMPPD (Lumi-Phos Plus; Lumigen PPD) is a chemiluminescent substrate for alkaline phosphatase (APase). AMPPD is hydrolyzed by APase to generate an unstable dioxetane intermediate, and the intermediate releases a chemiluminescent signal when it decomposes. The luminescent signal of AMPPD can be detected by highly sensitive equipment, thereby achieving quantitative analysis of the target molecule. AMPPD can be used in ultrasensitive enzyme-linked immunosorbent assays (such as quantitative detection of human tissue kininogen), chemiluminescent detection of proteins and nucleic acids, and other fields[1][2].

In Vitro

AMPPD (0.4 mM; 20 min) can be used as a substrate in a chemiluminescent enzyme-linked immunosorbent assay to detect human tissue kininogen. The results showed that the detection sensitivity can reach 1 pM, the calibration curve concentration range is 0.8 pM to 456 pM, and there is no interference from the biological matrix[1].
Compared with other chemiluminescent substrates (CSPD, LUMI-PHOS 530), AMPPD (0.4 mM; 20 min) produces comparable luminescent signal intensities for immunoreactive substances at the same concentration[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AMPPD Related Antibodies
Masse moléculaire

382.34

Formule

C18H23O7P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

COC1(C2=CC(OP(O)(O)=O)=CC=C2)C3(C(C4)CC5CC4CC3C5)OO1
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvant et solubilité
In Vitro: 

H2O : 175 mg/mL (457.71 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6155 mL 13.0774 mL 26.1547 mL
5 mM 0.5231 mL 2.6155 mL 5.2309 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation
Références

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.6155 mL 13.0774 mL 26.1547 mL 65.3868 mL
5 mM 0.5231 mL 2.6155 mL 5.2309 mL 13.0774 mL
10 mM 0.2615 mL 1.3077 mL 2.6155 mL 6.5387 mL
15 mM 0.1744 mL 0.8718 mL 1.7436 mL 4.3591 mL
20 mM 0.1308 mL 0.6539 mL 1.3077 mL 3.2693 mL
25 mM 0.1046 mL 0.5231 mL 1.0462 mL 2.6155 mL
30 mM 0.0872 mL 0.4359 mL 0.8718 mL 2.1796 mL
40 mM 0.0654 mL 0.3269 mL 0.6539 mL 1.6347 mL
50 mM 0.0523 mL 0.2615 mL 0.5231 mL 1.3077 mL
60 mM 0.0436 mL 0.2180 mL 0.4359 mL 1.0898 mL
80 mM 0.0327 mL 0.1635 mL 0.3269 mL 0.8173 mL
100 mM 0.0262 mL 0.1308 mL 0.2615 mL 0.6539 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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AMPPD Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AMPPD122341-56-4Lumi-Phos Plus Lumigen PPD PPDPhosphataseInhibitorinhibitorinhibit

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