2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. AMPPD

AMPPD  (Synonyms: Lumi-Phos Plus; Lumigen PPD; PPD)

製品番号: HY-15906 純度: 98.29%
COA 取扱説明書 Technical Support

AMPPD (Lumi-Phos Plus; Lumigen PPD) is a chemiluminescent substrate for alkaline phosphatase (APase). AMPPD is hydrolyzed by APase to generate an unstable dioxetane intermediate, and the intermediate releases a chemiluminescent signal when it decomposes. The luminescent signal of AMPPD can be detected by highly sensitive equipment, thereby achieving quantitative analysis of the target molecule. AMPPD can be used in ultrasensitive enzyme-linked immunosorbent assays (such as quantitative detection of human tissue kininogen), chemiluminescent detection of proteins and nucleic acids, and other fields.

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AMPPD

AMPPD 構造式

CAS 番号 : 122341-56-4

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 49 在庫あり
Solution
10 mM * 1 mL in Water USD 49 在庫あり
Solid
5 mg $45 在庫あり
10 mg $60 在庫あり
25 mg $95 在庫あり
50 mg $140 在庫あり
100 mg $200 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 1 publication(s) in Google Scholar

AMPPD 関連抗体

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  • 純度とドキュメンテーション

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製品説明

AMPPD (Lumi-Phos Plus; Lumigen PPD) is a chemiluminescent substrate for alkaline phosphatase (APase). AMPPD is hydrolyzed by APase to generate an unstable dioxetane intermediate, and the intermediate releases a chemiluminescent signal when it decomposes. The luminescent signal of AMPPD can be detected by highly sensitive equipment, thereby achieving quantitative analysis of the target molecule. AMPPD can be used in ultrasensitive enzyme-linked immunosorbent assays (such as quantitative detection of human tissue kininogen), chemiluminescent detection of proteins and nucleic acids, and other fields[1][2].

体外実験

AMPPD (0.4 mM; 20 min) can be used as a substrate in a chemiluminescent enzyme-linked immunosorbent assay to detect human tissue kininogen. The results showed that the detection sensitivity can reach 1 pM, the calibration curve concentration range is 0.8 pM to 456 pM, and there is no interference from the biological matrix[1].
Compared with other chemiluminescent substrates (CSPD, LUMI-PHOS 530), AMPPD (0.4 mM; 20 min) produces comparable luminescent signal intensities for immunoreactive substances at the same concentration[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AMPPD 関連抗体
分子量

382.34

分子式

C18H23O7P
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

COC1(C2=CC(OP(O)(O)=O)=CC=C2)C3(C(C4)CC5CC4CC3C5)OO1
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

H2O : 175 mg/mL (457.71 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6155 mL 13.0774 mL 26.1547 mL
5 mM 0.5231 mL 2.6155 mL 5.2309 mL
10 mM 0.2615 mL 1.3077 mL 2.6155 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

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体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.6155 mL 13.0774 mL 26.1547 mL 65.3868 mL
5 mM 0.5231 mL 2.6155 mL 5.2309 mL 13.0774 mL
10 mM 0.2615 mL 1.3077 mL 2.6155 mL 6.5387 mL
15 mM 0.1744 mL 0.8718 mL 1.7436 mL 4.3591 mL
20 mM 0.1308 mL 0.6539 mL 1.3077 mL 3.2693 mL
25 mM 0.1046 mL 0.5231 mL 1.0462 mL 2.6155 mL
30 mM 0.0872 mL 0.4359 mL 0.8718 mL 2.1796 mL
40 mM 0.0654 mL 0.3269 mL 0.6539 mL 1.6347 mL
50 mM 0.0523 mL 0.2615 mL 0.5231 mL 1.3077 mL
60 mM 0.0436 mL 0.2180 mL 0.4359 mL 1.0898 mL
80 mM 0.0327 mL 0.1635 mL 0.3269 mL 0.8173 mL
100 mM 0.0262 mL 0.1308 mL 0.2615 mL 0.6539 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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AMPPD Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AMPPD122341-56-4Lumi-Phos Plus Lumigen PPD PPDPhosphataseInhibitorinhibitorinhibit

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