1. Biochemical Assay Reagents

BAPTA 

Cat. No.: HY-100168 Purity: >98.0%
Handling Instructions

BAPTA is a specific chelator of Ca2+, suppresses intracellular reactive oxygen species (ROS) levels.

For research use only. We do not sell to patients.

BAPTA Chemical Structure

BAPTA Chemical Structure

CAS No. : 85233-19-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
100 mg USD 60 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
200 mg USD 72 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
500 mg USD 108 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
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5 g   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

BAPTA is a specific chelator of Ca2+, suppresses intracellular reactive oxygen species (ROS) levels.

IC50 & Target

Ca2+ chelator[1]

In Vitro

Regarding ROS generation, a Ca2+ specific chelator, BAPTA, suppresses ROS generation of Sodium lauryl sulfate (SLS)-exposed HaCaT keratinocytes[1]. Depolarization does not increase the resting open probability of the mechanoelectrical transducer (MET) current of Tmc1Bth/Bth OHCs, whereas raising the intracellular concentration of the Ca2+ chelator BAPTA causes smaller increases in resting open probability in Bthmutant outer hair cells (OHCs) than in wild-type control cells. In the presence of 0.1 mM BAPTA, nonsaturating bundle displacements causes the MET current to adapt in both genotypes, exactly as seen when 1 mM EGTA is used in the intracellular solution. In the presence of 10 mM intracellular BAPTA, the time-dependent MET current decline is abolished and the resting Popen increased to near 50% of the maximal MET current in OHCs from both Tmc1+/+ and Tmc1Bth/Bth mice. The relation between the MET current and bundle displacement shows that increasing the intracellular BAPTA concentration from 0.1 to 10 mM significantly increased (p<0.0001) the resting Popen of the MET current in both Tmc1+/+ (0.1 mM, 8±1.6%, n=4; 10 mM, 39.6±2.7%, n=5) and Tmc1Bth/Bth (0.1 mM, 10.4±2.2%, n=3; 10 mM, 46.5±9.9%, n=6). No significant differences are seen between the two genotypes for both BAPTA concentrations. However, 3 and 5 mM BAPTA are less effective in shifting the MET current-bundle displacement curves in Tmc1Bth/Bth than in Tmc1+/+ OHCs. In Tmc1+/+, increasing the BAPTA concentration from 0.1 mM to either 3 or 5 mM produces a highly significant increase in Popen (post hoc test from one-way ANOVA, p<0.01 and p<0.001, respectively); in Tmc1Bth/Bth, the same comparison produced no or a much reduced increase in Popen (n.s. and p<0.05, respectively)[2].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.0989 mL 10.4947 mL 20.9894 mL
5 mM 0.4198 mL 2.0989 mL 4.1979 mL
10 mM 0.2099 mL 1.0495 mL 2.0989 mL
Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

476.43

Formula

C₂₂H₂₄N₂O₁₀

CAS No.

85233-19-8

SMILES

O=C(O)CN(C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(O)=O)CC(O)=O)CC(O)=O

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 26 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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BAPTA
Cat. No.:
HY-100168
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