BAPTA
Based on 36 publication(s) in Google Scholar
BAPTA is a selective and cell-impermeant chelator for calcium. BAPTA has high selectivity against magnesium and calcium. BAPTA can also inhibit phospholipase C activity independently of their role as Ca2+ chelators.
For research use only. We do not sell to patients.
- Purity: 98.10%
- CAS No.: 85233-19-8
- Formula: C22H24N2O10
- Molecular Weight:476.43
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) BAPTA
More- Mil Med Res. 2023 Nov 25;10(1):56. [Abstract]
- Sci Immunol. 2019 Jun 28;4(36):eaau6426. [Abstract]
- Adv Sci (Weinh). 2021 Aug;8(15):e2100363. [Abstract]
- J Clin Invest. 2024 Apr 25;134(12):e176355. [Abstract]
- J Neuroinflammation. 2025 Aug 28;22(1):208. [Abstract]
- Environ Chem Ecotoxicol. 2025 Nov 19;8:267-280.
- Free Radic Biol Med. 2024 Mar:213:150-163. [Abstract]
- Sci Total Environ. 2020 Feb 10;703:134702. [Abstract]
- Biomed Pharmacother. 2023 Oct:166:115343. [Abstract]
- Biomed Pharmacother. 2023 May:161:114480. [Abstract]
- Cell Rep. 2025 Dec 10;44(12):116673. [Abstract]
- J Pineal Res. 2024 Aug;76(5):e12987. [Abstract]
- J Invest Dermatol. 2024 May 30:S0022-202X(24)00384-1. [Abstract]
- J Ginseng Res. 2022 Jul;46(4):515-525. [Abstract]
- J Ethnopharmacol. 2025 Apr 29:348:119863. [Abstract]
- J Ethnopharmacol. 2023 Feb 10;302(Pt A):115923. [Abstract]
- Int J Pharm. 2025 Nov 10:684:126125. [Abstract]
- J Thromb Haemost. 2021 Dec;19(12):3113-3125. [Abstract]
- Int J Mol Sci. 2026 Jan 1;27(1):468. [Abstract]
- Int Immunopharmacol. 2026 Mar 1:172:116168. [Abstract]
- Toxicology. 2023 Dec:500:153674. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2025 Jul 18;1871(8):167990. [Abstract]
- Exp Neurol. 2023 May:363:114350. [Abstract]
- J Cell Mol Med. 2020 Aug;24(16):9287-9299. [Abstract]
- J Funct Foods. 2024 Oct.
- J Funct Foods. December 2021, 104791.
- Aging. 2019 Nov 22;11(22):10385-10408. [Abstract]
- Cell Signal. 2025 Jul:131:111753. [Abstract]
- Mol Med Rep. 2024 Jan;29(1):13. [Abstract]
- Cardiovasc Ther. 2023 Mar 30:2023:3939360. [Abstract]
- J Cancer. 2024 Jan 1;15(2):343-355. [Abstract]
- J Pharm Pharmacol. 2024 May 3;76(5):567-578. [Abstract]
- Vet Microbiol. 2019 Dec;239:108498. [Abstract]
- Toxicon. 2026 Jun 15:276:109072. [Abstract]
- Res Sq. 2025 Apr 18.
- bioRxiv. 2023 Jun 25.
Customer Validation & Images
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WB
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IF
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RT-PCR
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ELISA
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IF
All Phospholipase Isoforms
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Biological Activity
Calcium[1]
BAPTA is widely used as an intracellular buffer to investigate the effects caused by Ca2+ release from intracellular calcium stores or Ca2+ influx via Ca2+-permeable channels in the plasma membrane[1].
BAPTA can be introduced into cells via methods such as patch-clamp and microinjection[1].
BAPTA inhibits PLC activity in isolated Drosophila melanogaster photoreceptor cells with an IC50 of approximately 8 mM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 85233-19-8
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Appearance Solid
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Molecular Weight 476.43
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Formula C22H24N2O10
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Color Off-white to light brown
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SMILES
O=C(O)CN(C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(O)=O)CC(O)=O)CC(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (36)
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Journal Impact Factor
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Most Recent
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Mil Med Res
Targeting GPR65 alleviates hepatic inflammation and fibrosis by suppressing the JNK and NF-κB pathways. [Abstract]2023 Nov 25;10(1):56. PMID: 38001521
BAPTA purchased from MedChemExpress. Usage Cited in: Mil Med Res. 2023 Nov 25;10(1):56. [Abstract]
BAPTA (10 μmol/L, 24 h). qRT-PCR was used to assess the mRNA level of Il6, Tnfα and Tgfβ3 in HMs.
BAPTA purchased from MedChemExpress. Usage Cited in: Mil Med Res. 2023 Nov 25;10(1):56. [Abstract]
BAPTA (10 μmol/L, 24 h). TGF-β1 and IL-6 level in the supernatant of HMs were detected by ELISA.
BAPTA purchased from MedChemExpress. Usage Cited in: Mil Med Res. 2023 Nov 25;10(1):56. [Abstract]
BAPTA (10 μmol/L, 24 h). The expression and location of TNF-α in HMs was assessed by confocal microscopy (c). Scale bar = 20 μm.
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Sci Immunol
2019 Jun 28;4(36):eaau6426. PMID: 31253642 -
Adv Sci (Weinh)
2021 Aug;8(15):e2100363. PMID: 34047068 -
J Clin Invest
Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer. [Abstract]2024 Apr 25;134(12):e176355. PMID: 38662435 -
J Neuroinflammation
P2X7-CaMKII drives 5-LOX nuclear translocation to impair microglial function after subarachnoid hemorrhage. [Abstract]2025 Aug 28;22(1):208. PMID: 40877894
BAPTA purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Aug 28;22(1):208. [Abstract]
BAPTA (10 μM). WB results showed that BAPTA could attenuate the Hb-induced decrease in LAMP1 and m-CTSD/pro-CTSD.
BAPTA purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Aug 28;22(1):208. [Abstract]
IF results showed that BAPTA (10 μM) can partially increase the co-localization coefficients of LAMP1 and CTSD.
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Free Radic Biol Med
Quercetin induces ferroptosis in gastric cancer cells by targeting SLC1A5 and regulating the p-Camk2/p-DRP1 and NRF2/GPX4 Axes. [Abstract]2024 Mar:213:150-163. PMID: 38190923 -
Sci Total Environ
Microcystin-leucine-arginine induced neurotoxicity by initiating mitochondrial fission in hippocampal neurons. [Abstract]2020 Feb 10;703:134702. PMID: 31753492 -
Biomed Pharmacother
Barasertib impedes chondrocyte senescence and alleviates osteoarthritis by mitigating the destabilization of heterochromatin induced by AURKB. [Abstract]2023 Oct:166:115343. PMID: 37634474 -
Biomed Pharmacother
Interruption of TRPC6-NFATC1 signaling inhibits NADPH oxidase 4 and VSMCs phenotypic switch in intracranial aneurysm. [Abstract]2023 May:161:114480. PMID: 37002575 -
Cell Rep
Aryl hydrocarbon receptor-induced activation of AIM2 inflammasome is mediated by MOMP and MPT: A vital therapeutic pathway for inflammation. [Abstract]2025 Dec 10;44(12):116673. PMID: 41385364 -
J Pineal Res
2024 Aug;76(5):e12987. PMID: 38975671 -
J Invest Dermatol
TRPV3-Activated PARP1/AIFM1/MIF Axis through Oxidative Stress Contributes to Atopic Dermatitis. [Abstract]2024 May 30:S0022-202X(24)00384-1. PMID: 38823435 -
J Ginseng Res
Ginsenoside Rg1 attenuates cerebral ischemia-reperfusion injury due to inhibition of NOX2-mediated calcium homeostasis dysregulation in mice. [Abstract]2022 Jul;46(4):515-525. PMID: 35818419 -
J Ethnopharmacol
Ginsenoside Rg1 attenuates T2DM-induced renal damage and fibrosis by inhibiting TRPC6-ChREBP-TXNIP signaling. [Abstract]2025 Apr 29:348:119863. PMID: 40311716 -
J Ethnopharmacol
Ginsenoside Rg1 attenuates glomerular fibrosis by inhibiting CD36/TRPC6/NFAT2 signaling in type 2 diabetes mellitus mice. [Abstract]2023 Feb 10;302(Pt A):115923. PMID: 36375645 -
Int J Pharm
Reversible increased basement membrane permeability and calcium ion redistribution facilitate ultrasound-enhanced transdermal drug delivery efficiency. [Abstract]2025 Nov 10:684:126125. PMID: 40914558 -
J Thromb Haemost
2021 Dec;19(12):3113-3125. PMID: 34411418 -
Int J Mol Sci
Gut Microbiota-Derived Propionic Acid Mediates ApoA-I-Induced Amelioration of MASLD via Activation of GPR43-Ca2+-CAMKII-ATGL Hepatic Lipolysis. [Abstract]2026 Jan 1;27(1):468. PMID: 41516340 -
Int Immunopharmacol
Piezo1 activates Wnt5a/FZD4 signaling to promote osteogenesis and mitigates age-associated bone loss. [Abstract]2026 Mar 1:172:116168. PMID: 41529412 -
Toxicology
Nonivamide inhibits proliferation of human corneal epithelial cells by inducing cell cycle arrest and oxidative stress. [Abstract]2023 Dec:500:153674. PMID: 37989449 -
Biochim Biophys Acta Mol Basis Dis
Activation of IRF2 signaling networks facilitates podocyte pyroptosis in lupus nephritis. [Abstract]2025 Jul 18;1871(8):167990. PMID: 40684959 -
Exp Neurol
Trpc6 knockout improves behavioral dysfunction and reduces Aβ production by inhibiting CN-NFAT1 signaling in T2DM mice. [Abstract]2023 May:363:114350. PMID: 36791875 -
J Cell Mol Med
Calpain-2 plays a pivotal role in the inhibitory effects of propofol against TNF-α-induced autophagy in mouse hippocampal neurons. [Abstract]2020 Aug;24(16):9287-9299. PMID: 32627970 -
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Aging
Rotating magnetic field delays human umbilical vein endothelial cell aging and prolongs the lifespan of Caenorhabditis elegans. [Abstract]2019 Nov 22;11(22):10385-10408. PMID: 31757933 -
Cell Signal
Rosuvastatin inhibits carcinogenesis through Ca2+ triggered endoplasmic reticulum stress pathway in pancreatic cancer. [Abstract]2025 Jul:131:111753. PMID: 40107481 -
Mol Med Rep
Trpc6 knockout protects against renal fibrosis by restraining the CN‑NFAT2 signaling pathway in T2DM mice. [Abstract]2024 Jan;29(1):13. PMID: 38038121 -
Cardiovasc Ther
KCa3.1 Promotes Proinflammatory Exosome Secretion by Activating AKT/Rab27a in Atrial Myocytes during Rapid Pacing. [Abstract]2023 Mar 30:2023:3939360. PMID: 37035755 -
J Cancer
JS-K activates G2/M checkpoints through the DNA damage response and induces autophagy via CAMKKβ/AMPKα/mTOR pathway in bladder cancer cells. [Abstract]2024 Jan 1;15(2):343-355. PMID: 38169515 -
J Pharm Pharmacol
2024 May 3;76(5):567-578. PMID: 38271051 -
Vet Microbiol
S100A9 regulates porcine reproductive and respiratory syndrome virus replication by interacting with the viral nucleocapsid protein. [Abstract]2019 Dec;239:108498. PMID: 31767072 -
Toxicon
Transient receptor potential canonical 6 is critical for chronic lipopolysaccharide exposure-induced pulmonary injury and fibrosis. [Abstract]2026 Jun 15:276:109072. PMID: 41850440 -
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Solvent & Solubility
1M NaOH : 33.33 mg/mL (69.96 mM; ultrasonic and adjust pH to 11 with NaOH)
DMSO : 15.62 mg/mL (32.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.56 mg/mL (3.27 mM); Clear solution
This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.56 mg/mL (3.27 mM); Clear solution
This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. R Y Tsien, et al. New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures. Biochemistry. 1980 May 27;19(11):2396-404. [Content Brief]
[2]. Roger C Hardie, et al. Inhibition of phospholipase C activity in Drosophila photoreceptors by 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid (BAPTA) and di-bromo BAPTA. Cell Calcium. 2005 Dec;38(6):547-56. [Content Brief]
[3]. M B Collatz, et al. Intracellular calcium chelator BAPTA protects cells against toxic calcium overload but also alters physiological calcium responses. Cell Calcium [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / 1M NaOH | 1 mM | 2.0989 mL | 10.4947 mL | 20.9894 mL | 52.4736 mL |
| 5 mM | 0.4198 mL | 2.0989 mL | 4.1979 mL | 10.4947 mL | |
| 10 mM | 0.2099 mL | 1.0495 mL | 2.0989 mL | 5.2474 mL | |
| 15 mM | 0.1399 mL | 0.6996 mL | 1.3993 mL | 3.4982 mL | |
| 20 mM | 0.1049 mL | 0.5247 mL | 1.0495 mL | 2.6237 mL | |
| 25 mM | 0.0840 mL | 0.4198 mL | 0.8396 mL | 2.0989 mL | |
| 30 mM | 0.0700 mL | 0.3498 mL | 0.6996 mL | 1.7491 mL | |
| 1M NaOH | 40 mM | 0.0525 mL | 0.2624 mL | 0.5247 mL | 1.3118 mL |
| 50 mM | 0.0420 mL | 0.2099 mL | 0.4198 mL | 1.0495 mL | |
| 60 mM | 0.0350 mL | 0.1749 mL | 0.3498 mL | 0.8746 mL |
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